Extremely low-frequency electromagnetic field exposure alters DNA methylation levels in the endometrium of pigs during the peri-implantation period.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study exposed porcine endometrial slices from the peri-implantation period (days 15–16 of pregnancy) to 50 Hz ELF-EMF for 2 hours and assessed promoter DNA methylation in selected genes. The authors report increased methylation for EGR2, ID2, and PTGER4, decreased methylation for IL1RAP and NOS3, and no…
Synergistic cytotoxic effects of an extremely low-frequency electromagnetic field with doxorubicin on MCF-7 cell line.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study tested whether a 50 Hz, 20 mT extremely low-frequency electromagnetic field (ELF-EMF) enhances the cytotoxic effects of doxorubicin (DOX) in the MCF-7 breast cancer cell line. The authors report that combining ELF-EMF with low-dose DOX reduced cell viability at lower DOX concentrations than DOX…
50 Hz Magnetic Field Exposure Inhibited Spontaneous Movement of Zebrafish Larvae through ROS-Mediated syn2a Expression.
Research
RF Safe Research Library
Jan 1, 2023
This zebrafish study exposed embryos to a 50 Hz magnetic field at 100–800 μT for 1 h or 24 h daily over 5 days. The authors report no changes in hatching, mortality, or malformation rates, but report reduced spontaneous movement at 200 μT along with brain histological abnormalities. They also report increased ROS and…
Effect of extremely low frequency electromagnetic field on the pathogenicity of Magnaporthe oryzae.
Research
RF Safe Research Library
Jan 1, 2023
This laboratory study examined Magnaporthe oryzae pathogenicity under extremely low frequency electromagnetic field exposure (50 Hz, 3 mT). The authors report increased infection ability, growth rate, and conidia generation with ELF-EMF exposure. They also report increased tolerance to a cell wall-perturbing agent…
Comparative Study of Extremely Low-Frequency Electromagnetic Field, Radiation, and Temozolomide Administration in Spheroid and Monolayer Forms of the Glioblastoma Cell Line (T98).
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RF Safe Research Library
Jan 1, 2023
This in vitro study assessed 50 Hz, 100 G extremely low-frequency EMF in combination with temozolomide and radiation in T98 glioblastoma cells grown as monolayers and spheroids. Radiation plus EMF increased apoptosis-related gene expression, while temozolomide plus EMF increased autophagy marker expression compared…
Effects of repeated exposure to 50 Hz electromagnetic field on breast cancer cells.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study exposed two human breast cancer cell lines to a 50 Hz ELF-EMF (20 mT) for 3 hours/day for up to four days. The authors report reduced viability and apoptosis-related findings in MDA-MB-231 cells, with no significant viability change in MCF-7 cells. Cell-cycle effects differed by cell line,…
Hazardous effects of high voltage electromagnetic field on albino rats and protective role of Rosmarinus officinalis.
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RF Safe Research Library
Jan 1, 2022
This animal study examined effects of a 50 Hz high-voltage electromagnetic field (5.4 kV/m) applied 2 or 4 hours per day for 25 days in albino rats. The authors report decreases in RBC, haemoglobin, and catalase activity, with increases in WBCs, liver enzymes, bilirubin, kidney-related markers, and malondialdehyde…
Effects of prolonged exposure to ELF-EMF on HERVs expression in human melanoma cells.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined whether 96-hour exposure to 50 Hz ELF-EMF affects expression of human endogenous retroviruses (HERV-H, HERV-K, HERV-W) in SK-MEL-37 human melanoma cells. Cells were continuously exposed at 1.5 or 3 mT and HERV env mRNA levels were measured by qPCR. The authors report significant…
Static and Electromagnetic Fields Differently Affect Proliferation and Cell Death Through Acid Enhancement of ROS Generation in Mesenchymal Stem Cells.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined how a 20 mT static magnetic field and a 20 mT 50 Hz electromagnetic field affect mesenchymal stem cells under conditions involving ROS and acidic pH. The authors report increased ROS and antioxidant-response markers (Nrf2, SOD2, GST) across SMF, EMF, and combined exposures, with…
Bidirectional Effect of Repeated Exposure to Extremely Low-Frequency Electromagnetic Field (50 Hz) of 1 and 7 mT on Oxidative/Antioxidative Status in Rat's Brain: The Prediction for the Vulnerability to Diseases.
Research
RF Safe Research Library
Jan 1, 2022
This rat study examined repeated 50 Hz ELF-EMF exposure (1 or 7 mT) and measured oxidative stress and antioxidant defense markers in the prefrontal cortex, including responses to a subsequent open-field stressor. The authors report intensity- and repetition-dependent effects: 1 mT produced weak changes and was…
The Short-Term Effect of Occupational Levels of 50 Hz Electromagnetic Field on Human Heart Rate Variability.
Research
RF Safe Research Library
Jan 1, 2021
This double-blind randomized sham-controlled repeated-measures study examined short-term 50 Hz ELF-EMF exposure (28 μT) to the chest and its effects on HR and HRV in 34 healthy young men. HR deceleration occurred in both EMF and sham conditions, but several HRV indices (SDNN, RMSSD, LF, HF) increased only in the EMF…
Conformational changes of β-thalassemia major hemoglobin and oxidative status of plasma after in vitro exposure to extremely low-frequency electromagnetic fields: An artificial neural network analysis.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed blood samples from β-thalassemia major patients and matched healthy donors (n=12 each) to 50 Hz ELF-EMF at 0.5 and 1 mT for 1–2 hours. The authors report that two-hour exposure induced significant oxidative changes in β-thalassemia major samples and examined protein/Hb structural features…
Pulsed and Discontinuous Electromagnetic Field Exposure Decreases Temozolomide Resistance in Glioblastoma by Modulating the Expression of O-Methylguanine-DNA Methyltransferase, Cyclin-D1, and p53.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study evaluated 50 Hz, 70 G electromagnetic field exposure in combination with temozolomide (TMZ) in T98 and A172 glioblastoma cell lines. The authors report that EMF plus TMZ increased cell death and reduced migration potential compared with controls. They also report increased p53 gene expression and…
Comparing the Effects of Long-term Exposure to Extremely Low-frequency Electromagnetic Fields With Different Values on Learning, Memory, Anxiety, and β-amyloid Deposition in Adult Rats.
Research
RF Safe Research Library
Jan 1, 2021
This animal study exposed adult male rats to 50 Hz ELF-EMF at 1, 100, 500, or 2000 μT for 1 hour/day over two months and assessed behavior and brain markers. The authors report increased memory retention (higher step-through latency) alongside increased anxiety-like behavior (reduced open-arm entries), particularly…
First Identification of the Effects of Low Frequency Electromagnetic Field on the Micromolecular Changes in Adipose Tissue-Derived Mesenchymal Stem Cells by Fourier Transform Infrared Spectroscopy.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed adipose tissue-derived mesenchymal stem cells to a continuous 50 Hz electromagnetic field at 1.5 mT for 24, 48, or 72 hours and assessed micromolecular changes using FTIR spectroscopy. The authors report the largest FTIR spectral differences versus control in nucleic acids and proteins…
Effects induced by a 50 Hz electromagnetic field and doxorubicin on Walker-256 carcinosarcoma growth and hepatic redox state in rats.
Research
RF Safe Research Library
Jan 1, 2021
This animal study compared 50 Hz extremely low-frequency EMF exposure with doxorubicin (and their combination) in Walker-256 carcinosarcoma-bearing rats, assessing tumor growth and liver redox-related outcomes. DOX and DOX+EMF were reported to inhibit tumor growth more than EMF alone, though EMF alone also showed an…
The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed mouse spermatogonial stem cells to 50 Hz EMF (2.5 mT; 1 h/day for 5 days) and assessed oxidative stress and cell health outcomes. The authors report increased ROS and apoptosis with reduced antioxidant (catalase) expression, viability, and colonization after EMF exposure. Sertoli…
Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…
Electromagnetic Field (EMF) Radiation Alters Estrogen Release from the Pig Myometrium during the Peri-Implantation Period.
Research
RF Safe Research Library
Jan 1, 2021
This ex vivo animal-tissue study tested whether ELF-EMF exposure (50 or 120 Hz for 2 or 4 hours) alters estrogenic activity in pig myometrium during the peri-implantation period. The authors report EMF-associated changes in estrogen-related gene transcripts and aromatase abundance, with progesterone diminishing some…
Evaluation of the neuroprotective effects of Vitamin E on the rat substantia nigra neural cells exposed to electromagnetic field: An ultrastructural study.
Research
RF Safe Research Library
Jan 1, 2021
This rat study exposed animals to a 50 Hz, 3 mT electromagnetic field and assessed oxidative stress markers and substantia nigra ultrastructure. EMF exposure was associated with higher lipid peroxidation (MDA), lower SOD activity, and TEM-detected changes in nucleus/nuclear membrane and myelin sheath. Vitamin E…
Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…
Extremely low frequency electromagnetic fields promote cognitive function and hippocampal neurogenesis of rats with cerebral ischemia.
Research
RF Safe Research Library
Jan 1, 2021
This animal study evaluated whether ELF-EMF exposure affects recovery-related outcomes after cerebral ischemia in rats. Rats exposed to 50 Hz, 1 mT ELF-EMF for 2 hours daily over 28 days showed better Morris water maze performance than untreated rats. The study also reports increased hippocampal neurogenesis markers…
Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study examined 50 Hz, 1 mT ELF-EMF exposure in LPS-treated THP-1 cells for 1–24 hours. The authors report increased HO-1 expression and Nrf2-related signaling, with nuclear enrichment of HO-1 and modulation of SIRT1/NF-κB pathways. They interpret these molecular changes as suggesting a protective,…
Enhancement in the ATP level and antioxidant capacity of under continuous exposure to extremely low-frequency electromagnetic field for multiple generations.
Research
RF Safe Research Library
Jan 1, 2020
This multi-generation animal study exposed worms continuously to 50 Hz, 3 mT extremely low-frequency electromagnetic fields and assessed outcomes in the 15th generation. The study reports increased body length, higher ATP content and ATP synthase activity, and upregulated ATP-related gene expression in exposed worms.…
Treating honey bees with an extremely low frequency electromagnetic field and pesticides: Impact on the rate of disappearance of azoxystrobin and λ-cyhalothrin and the structure of some functional groups of the probabilistic molecules.
Research
RF Safe Research Library
Jan 1, 2020
This laboratory study examined whether 50 Hz (ELF) electromagnetic field exposure changes pesticide disappearance rates and molecular functional group signatures in honey bees. EMF altered the disappearance rates of azoxystrobin and λ-cyhalothrin within 6 hours, with different patterns depending on whether pesticides…