The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.
This in vitro study exposed mouse spermatogonial stem cells to 50 Hz EMF (2.5 mT; 1 h/day for 5 days) and assessed oxidative stress and cell health outcomes. The authors report increased ROS and apoptosis with reduced antioxidant (catalase) expression, viability, and colonization after EMF exposure. Sertoli cell-derived exosomes were reported to mitigate these changes, suggesting a potential protective role via oxidative stress regulation.
Key points
- SSCs were exposed in vitro to 50 Hz EMF at 2.5 mT for 1 hour over 5 days.
- EMF exposure was associated with ROS accumulation and reduced catalase antioxidant expression.
- EMF-exposed SSCs showed reduced viability and colonization efficiency compared with controls.
- Apoptosis increased after EMF exposure, supported by AO/PI staining and flow cytometry.
- Gene expression changes included Caspase-3 up-regulation and GFRα1 down-regulation with EMF exposure.
- Sertoli-derived exosomes were taken up by SSCs and were reported to ameliorate EMF-associated oxidative stress and apoptosis-related changes.
Referenced studies & papers
Relevant papers in OpenMel
Source:
Open original
AI-generated summaries may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.
AI-generated summaries may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.
Comments
Log in to comment.
No comments yet.