Extremely low frequency electromagnetic fields promote mesenchymal stem cell migration by increasing intracellular Ca and activating the FAK/Rho GTPases signaling pathways in vitro.
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RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human bone marrow-derived mesenchymal stem cells to extremely low frequency EMF (7.5–70 Hz, 1 mT) generated by Helmholtz coils. The authors report increased MSC migration across all tested frequencies. Mechanistically, the effect is described as dependent on intracellular calcium and…
Selenium supplementation ameliorates electromagnetic field-induced oxidative stress in the HEK293 cells.
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RF Safe Research Library
Jan 1, 2018
This in vitro study examined whether selenium supplementation modifies oxidative stress and apoptosis markers in HEK293 cells exposed to 2.4 GHz EMF. The authors report that EMF exposure increased lipid peroxidation (MDA) and apoptosis-related markers while decreasing antioxidant enzyme activities and bcl-2 versus…
Expressions of some antioxidant genes in SH-SY5Y cells treated with β-lapachone, morphine and electromagnetic field.
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RF Safe Research Library
Jan 1, 2018
This in vitro study exposed SH-SY5Y cells to a 50 Hz, 0.50 mT electromagnetic field under intermittent or continuous conditions, alongside β-lapachone and/or morphine. The authors report condition-dependent changes in antioxidant gene expression, including decreased NQO1 and increased NQO2 under intermittent EMF…
Effects of Fifty-Hertz Electromagnetic Fields on Granulocytic Differentiation of ATRA-Treated Acute Promyelocytic Leukemia NB4 Cells.
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Jan 1, 2018
This in vitro study examined whether 50 Hz, 2 mT extremely low-frequency electromagnetic fields affect ATRA-driven granulocytic differentiation in NB4 acute promyelocytic leukemia cells. The authors report reduced proliferation and enhanced maturation/differentiation markers with ELF-EMF exposure. They also report…
Extremely low frequency variable electromagnetic fields affect cancer and noncancerous cells in vitro differently: Preliminary study.
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RF Safe Research Library
Jan 1, 2018
This in vitro study exposed cancer (U87-MG; 143B) and noncancerous (BJ; HEK) cells to extremely low frequency electromagnetic fields under varying frequencies (2–60 Hz), waveforms, and exposure durations, with sham controls. Cell viability was assessed using an MTT assay. The authors report that cancer and…
Gene Expression Analysis in Human Peripheral Blood Cells after 900 MHz RF-EMF Short-Term Exposure.
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RF Safe Research Library
Jan 1, 2018
This ex vivo/in vitro study exposed peripheral blood cells from five human donors to a 900 MHz continuous-wave RF-EMF for up to 90 minutes (average SAR 9.3 W/kg). After false discovery rate adjustment, the authors report no EMF-specific gene expression changes on microarray analysis. Although low-stringency analysis…
Electromagnetic Field Could Protect SH-SY5Y Cells Against Cisplatin Cytotoxicity, But Not MCF-7 Cells.
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Jan 1, 2018
This in vitro study exposed MCF-7 and SH-SY5Y cells to a 50 Hz, 0.50 mT electromagnetic field in combination with cisplatin and/or morphine. The combined "CDDP+Mor+EMF" condition showed higher cisplatin IC values than cisplatin alone and "CDDP+Mor" in both cell lines, consistent with reduced cisplatin cytotoxicity.…
Effects of 1800 MHz RF-EMF exposure on DNA damage and cellular functions in primary cultured neurogenic cells.
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Jan 1, 2018
This in vitro study exposed primary cultured astrocytes, microglia, and cortical neurons to 1800 MHz RF-EMF at 4.0 W/kg SAR to assess DNA damage and cellular functions. The authors report no significant induction of γH2AX foci and no significant changes in cytokine secretion or dendrite/synapse morphological…
Extremely low-frequency electromagnetic fields accelerates wound healing modulating MMP-9 and inflammatory cytokines.
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Jan 1, 2018
This in vitro study used a scratch wound model in human immortalized keratinocytes (HaCaT) to examine effects of extremely low-frequency electromagnetic fields (ELF-EMF). ELF-EMF exposure was reported to accelerate wound closure compared with sham and to increase inflammatory cytokine expression/release. The authors…
Extremely low-frequency electromagnetic field (EMF) generates alterations in the synthesis and secretion of oestradiol-17β (E) in uterine tissues: An in vitro study.
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Jan 1, 2018
This in vitro study exposed porcine endometrial and myometrial slices to extremely low-frequency EMF (50 or 120 Hz, 8 mT) for 2 or 4 hours, with or without progesterone. The authors report frequency- and time-dependent changes in oestradiol-17β secretion and CYP19A3 mRNA expression, with fewer changes when…
Effects of 1950 MHz radiofrequency electromagnetic fields on Aβ processing in human neuroblastoma and mouse hippocampal neuronal cells.
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Jan 1, 2018
This in vitro study exposed HT22 mouse hippocampal neuronal cells and SH-SY5Y human neuroblastoma cells to 1950 MHz RF-EMF at SAR 6 W/kg for 2 hours per day over 3 days. RF-EMF exposure reduced mRNA levels of several Aβ-processing genes in HT22 cells, but did not change APP mRNA in SH-SY5Y cells. Protein levels of…
Structural features of hydroxyapatite and carbonated apatite formed under the influence of ultrasound and microwave radiation and their effect on the bioactivity of the nanomaterials.
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RF Safe Research Library
Jan 1, 2018
This study reports synthesizing hydroxyapatite and carbonate-substituted hydroxyapatite using ultrasound and microwave radiation as processing methods. The authors report that these physical factors produced calcium-deficient hydroxyapatite and B-type carbonated apatite and increased in vitro bioactivity compared…
Genotoxic Effects in Human Fibroblasts Exposed to Microwave Radiation.
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RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human fetal and adult fibroblasts to 25 GHz microwave radiation and assessed multiple genotoxic and cellular endpoints. The authors report no evidence of direct DNA damage and no apoptosis or changes in prosurvival signalling proteins. However, they report increased total and…
Biodegradable, pH-sensitive chitosan beads obtained under microwave radiation for advanced cell culture.
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RF Safe Research Library
Jan 1, 2018
This in vitro study describes microwave-assisted, green-chemistry synthesis of pH-sensitive chitosan beads crosslinked with a glutamic acid/1,5-pentanodiol mixture and optionally modified with Tilia platyphyllos extract. The beads were characterized (FT-IR, SEM), tested for swelling, pH sensitivity, antioxidant…
Effects of radiofrequency radiation in the presence of gold nanoparticles for the treatment of renal cell carcinoma.
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RF Safe Research Library
Jan 1, 2017
This in vitro study evaluated radiofrequency (RF) radiation with and without gold nanoparticles in human embryonic kidney (HEK) cancer cells as a potential approach for renal cell carcinoma treatment. The authors report reduced cell survival in one RF-exposed group versus control, and additional reductions in…
The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells.
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Jan 1, 2017
This in vitro study compared ginsenosides extracted using pulsed electric field (PEF) versus solvent cold soak extraction (SCSE). The abstract reports that PEF extracts had higher antioxidant capacity and provided greater protection against hydrogen peroxide-induced oxidative stress in HEK-293 cells, including…
The effects of electromagnetic fields on B16-BL6 cells are dependent on their spatial and temporal character.
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Jan 1, 2017
This in vitro study tested several complex low-intensity, low-frequency EMF patterns on proliferation of B16-BL6 mouse melanoma cells. A specific pattern (Thomas-EMF) with a 3 ms point duration reportedly inhibited proliferation and increased calcium uptake, while other timing settings did not. The authors conclude…
RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells.
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RF Safe Research Library
Jan 1, 2017
This in vitro study assessed whether intermittent 1800 MHz RF-EMF exposure induces DNA damage or alters cellular behavior in three neurogenic cell lines. Cells were exposed at an average SAR of 4.0 W/kg for 1, 6, or 24 hours and evaluated for γH2AX foci, cell cycle progression, proliferation, and viability. The…
Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells.
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RF Safe Research Library
Jan 1, 2017
This in vitro study tested 50 Hz, 3 mT electromagnetic field (EMF) exposure and 808 nm low-level laser (LLL), alone and combined, on human adipose tissue-derived mesenchymal stem cells from six donors. The authors report increased cell numbers after 7 days with EMF, greater increases with LLL, and the largest…
Effect of extremely low-frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544.
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RF Safe Research Library
Jan 1, 2017
This in vitro study exposed human keratinocyte cells (NCTC 2544) to 50 Hz ELF-EMF at 0.25–2 G and assessed oxidative stress-related endpoints. Significant increases in ROS were reported at 0.5 and 1 G, with associated short-term changes in antioxidant and lipid peroxidation markers. The authors report that…
Influence of the on-line ELF-EMF stimulation on the electrophysiological properties of the rat hippocampal CA1 neurons in vitro.
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Jan 1, 2017
This paper describes an on-line ELF magnetic stimulation setup and evaluates coil field distribution using COMSOL simulations alongside physical measurements. The authors report that modeled and measured magnetic flux densities were similar, and that stimulation induced sodium channel currents and fEPSPs in rat…
The effect of a high frequency electromagnetic field in the microwave range on red blood cells.
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Jan 1, 2017
An in vitro study examined red blood cells exposed to an 18 GHz electromagnetic field and assessed uptake of nanospheres. The authors report that EMF exposure enabled nanosphere uptake into RBCs, with different translocation behavior for 23.5 nm versus 46.3 nm particles. Uptake was reported to increase with higher…
The expression of pluripotency and neuronal differentiation markers under the influence of electromagnetic field and nitric oxide.
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RF Safe Research Library
Jan 1, 2017
This in vitro study examined how 50 Hz low-frequency EMF and a nitric oxide donor (Deta-NO) affect stemness and neuronal differentiation markers in rat bone marrow mesenchymal stem cells. The authors report that high NO (1 mM) combined with EMF reduced stemness markers and increased neuronal differentiation markers,…
Effect of electromagnetic fields on human osteoarthritic and non-osteoarthritic chondrocytes.
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RF Safe Research Library
Jan 1, 2017
This in vitro study exposed human osteoarthritic and non-osteoarthritic chondrocyte pellets to a sinusoidal 15 Hz electromagnetic field at 5 mT or 8 mT for 7 days. Under 5 mT, osteoarthritic chondrocytes showed increased expression of cartilage-related genes (COL2A1 and ACAN) compared with controls, while…
Rat adipose-derived mesenchymal stem cells aging reduction by zinc sulfate under extremely low frequency electromagnetic field exposure is associated with increased telomerase reverse transcriptase gene expression.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study exposed rat adipose-derived mesenchymal stem cells to 50 Hz, 20 mT ELF-EMF for 30 minutes per day over 21 days, with and without zinc sulfate. The authors report ELF-EMF exposure decreased TERT gene expression and increased senescent cells. Zinc sulfate was reported to increase TERT expression and…