The expression of pluripotency and neuronal differentiation markers under the influence of electromagnetic field and nitric oxide.

AI: Melanie Research RF Safe Research Library Jan 1, 2017 NEUTRAL LOW

This in vitro study examined how 50 Hz low-frequency EMF and a nitric oxide donor (Deta-NO) affect stemness and neuronal differentiation markers in rat bone marrow mesenchymal stem cells. The authors report that high NO (1 mM) combined with EMF reduced stemness markers and increased neuronal differentiation markers, alongside reduced proliferation and morphology changes. Low NO (50 µM) was reported to reduce some neuronal differentiation markers (Nestin, DCX) without changing Oct4, which the authors interpret as maintaining stemness.

Key points

The study used rat bone marrow mesenchymal stem cells and assessed gene/protein markers by qPCR and immunocytochemistry.
Exposure included 50 Hz EMF and Deta-NO at 50 µM (low) or 1 mM (high).
High NO plus EMF was associated with down-regulation of stemness markers and up-regulation of neuronal differentiation markers.
High NO plus EMF was also associated with decreased cell proliferation and altered morphology.
Low NO was reported to decrease Nestin and DCX without changing Oct4.
The authors describe a concentration-dependent ("double behavior") effect of NO, with differentiation promoted under high NO plus EMF conditions.

Referenced studies & papers

Relevant papers in OpenMel

Source: Open original

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