Archive

This preprint describes an in vitro bioreactor-based system to test whether electromagnetic/electrical stimulation affects extracellular vesicles (EVs). Using proteomics and single-vesicle flow cytometry, the authors report that stimulation at physiological current amplitudes can modulate EV content in a…

This animal and laboratory study evaluated extremely low-frequency electromagnetic stimulation (ELF-EMS) in a rodent ischemia-reperfusion stroke model and related in vitro/slice experiments. The authors report improved neurological and behavioral outcomes, increased neuronal survival, and reduced glial/microglial…

This in vitro study tested ELF-EMF exposure in U87 and U251 glioblastoma cell lines and assessed apoptosis, cell-cycle distribution, cell size, and mRNA expression of cell-cycle genes. The authors report higher apoptosis induction and increased G2/M arrest in U87 compared with U251, alongside increased U87 cell size…

This in vitro study tested whether a 50 Hz, 20 mT extremely low-frequency electromagnetic field (ELF-EMF) enhances the cytotoxic effects of doxorubicin (DOX) in the MCF-7 breast cancer cell line. The authors report that combining ELF-EMF with low-dose DOX reduced cell viability at lower DOX concentrations than DOX…

This in vitro study compared continuous versus intermittent 16 Hz ELF-PEMF exposure strategies in osteoprogenitor (SCP-1) cells. Intermittent exposure (10 min every 8 h) was reported to enhance cell numbers and osteogenic function relative to continuous daily exposure (30 min every 24 h). The authors report increased…

This in vitro study assessed 50 Hz, 100 G extremely low-frequency EMF in combination with temozolomide and radiation in T98 glioblastoma cells grown as monolayers and spheroids. Radiation plus EMF increased apoptosis-related gene expression, while temozolomide plus EMF increased autophagy marker expression compared…

This review discusses low-energy amplitude-modulated RF EMF (27.12 MHz carrier with low Hz–kHz envelope modulation) delivered systemically via intrabuccal antenna devices as a potential treatment for advanced hepatocellular carcinoma. It reports preclinical inhibition of cancer growth in vitro and in mouse xenograft…

This in vitro study developed magnetic electrospun nanofibers for bone regeneration and evaluated osteogenic and antibacterial properties. Functionalization with ATP and Mg increased mesenchymal stem cell adhesion, proliferation, and osteogenic marker readouts compared with unmodified fibers. The authors report that…

This in vitro study exposed two human breast cancer cell lines to a 50 Hz ELF-EMF (20 mT) for 3 hours/day for up to four days. The authors report reduced viability and apoptosis-related findings in MDA-MB-231 cells, with no significant viability change in MCF-7 cells. Cell-cycle effects differed by cell line,…

This in vitro study examined whether a 2 GHz RF-EMF exposure could enhance enzyme production in a filamentous fungus. A 10-minute exposure was reported to increase extracellular total protein and α-amylase activity in culture media versus controls, alongside increased α-amylase mRNA and changes consistent with…

This in vitro study examined whether 96-hour exposure to 50 Hz ELF-EMF affects expression of human endogenous retroviruses (HERV-H, HERV-K, HERV-W) in SK-MEL-37 human melanoma cells. Cells were continuously exposed at 1.5 or 3 mT and HERV env mRNA levels were measured by qPCR. The authors report significant…

This in vitro study evaluated SAR and erythrocyte membrane stability during exposure to a fully textile wearable antenna at 2.41 GHz under realistic (6.3 mW) and conservative (450 mW) input power conditions. It reports 1 g average SARs of 0.5758 W/kg and 41.13 W/kg, respectively. For membrane stability at 6.3 mW,…

This in vitro study tested an ELF-EMF at a calculated thermal resonant frequency in 2D and 3D culture models of human pancreatic cancer, glioblastoma, and breast cancer. The authors report reduced cell growth and a metabolic shift, including potentiation of coupled and uncoupled respiration and altered transcription…

This in vitro study examined how a 20 mT static magnetic field and a 20 mT 50 Hz electromagnetic field affect mesenchymal stem cells under conditions involving ROS and acidic pH. The authors report increased ROS and antioxidant-response markers (Nrf2, SOD2, GST) across SMF, EMF, and combined exposures, with…

This in vitro study examined whether a low-frequency EMF (30 mT, 7 Hz for 3 h) alters phagocytosis of latex beads and immune-related protein/gene expression in the human Mono Mac 6 macrophage cell line. The authors report that EMF exposure influenced phagocytosis-associated molecular markers, with notable changes in…

This in vitro study examined intermittent extremely low-frequency electromagnetic field (ELF-EMF) exposure (6 h/day) in human periodontal ligament mesenchymal stem cells during osteogenic differentiation. After 10 days, ELF-EMF-exposed cells showed increased proliferation and calcium deposition versus sham.…

This in vitro study exposed human adipose-derived mesenchymal stem progenitor cells to an alternating EMF generated by the IteraCoil device at 0.05 and 1 kHz. The authors report that EMF exposure was associated with osteoblastic differentiation in the absence of exogenous pro-osteogenic supplements, assessed by…

This in vitro study evaluated whether radiofrequency electromagnetic field (RF-EMF) exposure could influence wound-healing-related processes. The authors report increased keratinocyte migration and changes in expression of wound-healing genes, including MMPs, TIMPs, and pro/anti-inflammatory cytokines. The abstract…

This in vitro study examined how low-frequency electromagnetic fields (LF-EMF) at different magnetic flux densities influence skeletal myogenesis in C2C12 cells. The authors report that 2.0 mT LF-EMF activated cells and increased PAX7, while 1.5 mT increased MyoD and myogenin expression. They conclude that different…

This in vitro study evaluated short-term extremely low frequency electromagnetic field (ELF-EMF) exposure and silver nanoparticles (AgNPs), alone and combined, for antibacterial effects and apoptosis induction in MCF-7 breast cancer cells. The authors report time-dependent reductions in bacterial viable counts, with…

This in vitro study tested continuous or pulsed ELF electromagnetic field exposure in human cancer cells treated with the anticancer agent 3-bromopyruvate (3BP). The authors report that ELF-EMF enhanced 3BP anti-cancer activity, which they link to reduced TNFα secretion, p21/p27-associated G2/M arrest, and cancer…