Electromagnetic field as a possible inhibitor of tumor invasion by declining E-cadherin/N-cadherin switching in triple negative breast cancer.
Research
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Jan 1, 2024
This in vitro study exposed breast cell lines to a 1 Hz, 100 mT ELF-EMF for 5 days (2 h/day) and assessed migration, invasion, and cadherin expression. The authors report reduced migration and invasion in MDA-MB231 cells versus sham exposure. They also report increased E-cadherin and decreased N-cadherin in MDA-MB231…
The exposure to extremely low frequency electromagnetic-fields inhibits the growth and potentiates the sensitivity to chemotherapy of bidimensional and tridimensional human osteosarcoma models.
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RF Safe Research Library
Jan 1, 2024
This in vitro study tested a specific extremely low frequency electromagnetic field (ELF-EMF) on three human osteosarcoma cell lines grown in 2D and 3D spheroid models. The abstract reports that ELF-EMF exposure inhibited proliferation and altered mitochondrial metabolism in the cancer models. It also reports…
Extremely low-frequency electromagnetic field (ELF-EMF) induced alterations in gene expression and cytokine secretion in clear cell renal carcinoma cells.
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Jan 1, 2024
This in vitro study exposed three ccRCC cell lines and a healthy kidney cell line (HEK293) to 50 Hz ELF-EMF at 4.5 mT for 30 minutes daily over 5 days. The authors report exposure-associated changes in expression of several genes (names not fully visible in the abstract), with differences between primary…
Comparison of pulsed and continuous electromagnetic field generated by WPT system on human dermal and neural cells.
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Jan 1, 2024
This in vitro study tested electromagnetic fields generated by a prototype wireless power transfer (wireless charging) system on four human dermal and neural cell lines. Across pulsed and continuous exposure conditions, the authors report no negative effects on morphology/cytoskeleton, viability/mitosis,…
Induction of apoptosis in B16-BL6 melanoma cells following exposure to electromagnetic fields modeled after intercellular calcium waves.
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Jan 1, 2024
This in vitro study exposed B16-BL6 melanoma cells to a time-varying EMF modeled after intercellular calcium-wave firing patterns. The abstract reports a 50.3% reduction in viable cells after a single 40-minute exposure and microscopy findings consistent with apoptosis and nuclear fragmentation versus control. The…
Spinning magnetic field patterns that cause oncolysis by oxidative stress in glioma cells
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Nov 7, 2023
This in vitro study characterized spinning oscillating magnetic field (sOMF) stimulation patterns that the authors report can induce cytotoxicity in two types of human glioma cells. The reported effect was linked to a critical rise in superoxide, and was reversed by the antioxidant Trolox, suggesting ROS involvement.…
Osteogenic effect of electromagnetic fields on stem cells derived from rat bone marrow cultured in osteogenic medium versus conditioned medium in vitro.
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RF Safe Research Library
Jan 1, 2023
This in vitro study tested whether ELF-EMF exposure (50 Hz, 1 mT; 30 min/day) influences osteogenic differentiation markers in rat bone-marrow-derived stem cells cultured in osteogenic medium or in human adipose-stem cell-conditioned medium. The OM + EMF condition showed higher ALP activity and higher Runx2 and Ocn…
Effects of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on glioblastoma cells (U -118 MG).
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Jan 1, 2023
This in vitro study exposed glioblastoma (U-118 MG) and human embryonic kidney (Hek-293) cells to 2.4 GHz RF-EMF for 24–72 hours, with or without a dielectric screen. The authors report a significant decrease in cell viability after exposure in both cell lines. The decrease was described as greater in glioblastoma…
The Effect of Low-Frequency Pulsed Electromagnetic Fields on the Differentiation of Permanent Dental Pulp Stem Cells into Odontoblasts.
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Jan 1, 2023
This in vitro study exposed dental pulp mesenchymal stem cells to 50 Hz pulsed electromagnetic fields at 0.5 or 1 mT for 20 or 40 minutes per day over seven days. Cell survival/proliferation was generally higher than control across exposure groups, except for the 40 minutes/day at 1 mT condition. DMP1 expression was…
Human osteosarcoma cells in response to ELF-MF: Morphological remodeling compared to cell proliferation.
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Jan 1, 2023
This in vitro study exposed human osteosarcoma (MG-63) cells to 2 mT extremely low-frequency magnetic fields with sinusoidal, triangular, or pulsed waveforms under continuous (3 h) or fractionated (3 days) conditions. The authors report significant decreases in proliferation and shape index in exposed cells versus…
Assessing the effect of selenium on cyclin D1 level and nuclear factor kappa b activity in NIH/3T3 fibroblast cells at 2100 MHz electromagnetic field exposure.
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Jan 1, 2023
This in vitro study examined NIH/3T3 fibroblast cells exposed to 2100 MHz EMF and evaluated cell viability, cyclin D1, and NF-κB activity, with and without 200 nM selenium. The abstract reports reduced cell viability and increased cyclin D1 and NF-κB activity after EMF exposure. Selenium supplementation was reported…
A Novel and Versatile Microfluidic Device for Cell Assays under Radio Frequency Exposure.
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Jan 1, 2023
This in vitro study describes a microfluidic device integrated with a radiofrequency exposure system to enable on-chip monitoring of cellular responses under a 1.8 GHz field. Using human amniotic epithelial cells, the authors report field-intensity-associated increases in protrusions/filopodia, changes in…
Phagocytosis of latex beads by a human monocytic Mono Mac 6 cell line and effects of low-frequency electromagnetic field interaction.
Research
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Jan 1, 2023
This in vitro study tested whether a low-frequency EMF (7 Hz, 30 mT) alters phagocytosis-related outcomes in the human monocytic Mono Mac 6 cell line. EMF exposure was associated with increased necrosis in cells pre-stimulated with SEB and then undergoing latex bead phagocytosis, while no significant cell-death…
Impact of ketamine administration on chronic unpredictable stress-induced rat model of depression during extremely low-frequency electromagnetic field exposure: Behavioral, histological and molecular study.
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Jan 1, 2023
This animal study evaluated ketamine administration combined with extremely low-frequency electromagnetic field (ELF-EMF) exposure in a chronic unpredictable stress (CUS) rat model. The authors report that the combined treatment increased depression-like behavior and neuronal degeneration, reduced GFAP(+) cells in…
Intermittent Exposure to a 16 Hz Extremely Low Frequency Pulsed Electromagnetic Field Promotes Osteogenesis In Vitro through Activating Piezo 1-Induced Ca Influx in Osteoprogenitor Cells.
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Jan 1, 2023
This in vitro study compared continuous versus intermittent 16 Hz ELF-PEMF exposure strategies in osteoprogenitor (SCP-1) cells. Intermittent exposure (10 min every 8 h) was reported to enhance cell numbers and osteogenic function relative to continuous daily exposure (30 min every 24 h). The authors report increased…
Comparative Study of Extremely Low-Frequency Electromagnetic Field, Radiation, and Temozolomide Administration in Spheroid and Monolayer Forms of the Glioblastoma Cell Line (T98).
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Jan 1, 2023
This in vitro study assessed 50 Hz, 100 G extremely low-frequency EMF in combination with temozolomide and radiation in T98 glioblastoma cells grown as monolayers and spheroids. Radiation plus EMF increased apoptosis-related gene expression, while temozolomide plus EMF increased autophagy marker expression compared…
Effects of repeated exposure to 50 Hz electromagnetic field on breast cancer cells.
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Jan 1, 2022
This in vitro study exposed two human breast cancer cell lines to a 50 Hz ELF-EMF (20 mT) for 3 hours/day for up to four days. The authors report reduced viability and apoptosis-related findings in MDA-MB-231 cells, with no significant viability change in MCF-7 cells. Cell-cycle effects differed by cell line,…
Effects of prolonged exposure to ELF-EMF on HERVs expression in human melanoma cells.
Research
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Jan 1, 2022
This in vitro study examined whether 96-hour exposure to 50 Hz ELF-EMF affects expression of human endogenous retroviruses (HERV-H, HERV-K, HERV-W) in SK-MEL-37 human melanoma cells. Cells were continuously exposed at 1.5 or 3 mT and HERV env mRNA levels were measured by qPCR. The authors report significant…
High-specificity protection against radiation-induced bone loss by a pulsed electromagnetic field.
Research
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Jan 1, 2022
This study reports a noninvasive pulsed-burst electromagnetic field (PEMF) approach intended to protect against radiotherapy-induced bone loss. A 15 Hz, 2 mT PEMF waveform is described as producing distinctive calcium oscillations in osteoblasts and inhibiting radiation-related bone loss while not affecting other…
Static and Electromagnetic Fields Differently Affect Proliferation and Cell Death Through Acid Enhancement of ROS Generation in Mesenchymal Stem Cells.
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Jan 1, 2022
This in vitro study examined how a 20 mT static magnetic field and a 20 mT 50 Hz electromagnetic field affect mesenchymal stem cells under conditions involving ROS and acidic pH. The authors report increased ROS and antioxidant-response markers (Nrf2, SOD2, GST) across SMF, EMF, and combined exposures, with…
Improved osteogenic differentiation by extremely low electromagnetic field exposure: possible application for bone engineering.
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Jan 1, 2022
This in vitro study examined intermittent extremely low-frequency electromagnetic field (ELF-EMF) exposure (6 h/day) in human periodontal ligament mesenchymal stem cells during osteogenic differentiation. After 10 days, ELF-EMF-exposed cells showed increased proliferation and calcium deposition versus sham.…
Cardiac Cell Exposure to Electromagnetic Fields: Focus on Oxdative Stress and Apoptosis.
Research
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Jan 1, 2022
This study assessed whether 915 MHz RF-EMF exposure alters oxidative stress and apoptosis markers in cardiac cells and mouse heart tissue. The authors report no statistically significant differences in apoptotic profile, antioxidant capacity, tissue integrity, or expression of apoptosis/antioxidant genes after…
Electromagnetic Fields Generated by the IteraCoil Device Differentiate Mesenchymal Stem Progenitor Cells Into the Osteogenic Lineage.
Research
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Jan 1, 2022
This in vitro study exposed human adipose-derived mesenchymal stem progenitor cells to an alternating EMF generated by the IteraCoil device at 0.05 and 1 kHz. The authors report that EMF exposure was associated with osteoblastic differentiation in the absence of exogenous pro-osteogenic supplements, assessed by…
Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined how low-frequency electromagnetic fields (LF-EMF) at different magnetic flux densities influence skeletal myogenesis in C2C12 cells. The authors report that 2.0 mT LF-EMF activated cells and increased PAX7, while 1.5 mT increased MyoD and myogenin expression. They conclude that different…
Anticancer and antibacterial potentials induced post short-term exposure to electromagnetic field and silver nanoparticles and related pathological and genetic alterations: in vitro study.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study evaluated short-term extremely low frequency electromagnetic field (ELF-EMF) exposure and silver nanoparticles (AgNPs), alone and combined, for antibacterial effects and apoptosis induction in MCF-7 breast cancer cells. The authors report time-dependent reductions in bacterial viable counts, with…