Static and Electromagnetic Fields Differently Affect Proliferation and Cell Death Through Acid Enhancement of ROS Generation in Mesenchymal Stem Cells.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined how a 20 mT static magnetic field and a 20 mT 50 Hz electromagnetic field affect mesenchymal stem cells under conditions involving ROS and acidic pH. The authors report increased ROS and antioxidant-response markers (Nrf2, SOD2, GST) across SMF, EMF, and combined exposures, with…
Morphological and cytophysiological changes in selected lines of normal and cancer human cells under the influence of a radio-frequency electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed human fibroblasts and prostate cancer cells to 2.5 GHz RF-EMF for 24, 48, and 72 hours and assessed viability and morphology. The authors report decreased viability in fibroblasts and increased viability in prostate cancer cells after exposure, while morphology showed no significant…
EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE.
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RF Safe Research Library
Jan 1, 2021
This in vitro study examined whether extremely low-frequency electromagnetic fields (ELF-EMF) at 0.2 or 2 mT (continuous or 1.5 h on/1.5 h off) for 18 hours alter viability and gene/miRNA expression in AGS gastric cancer cells. The authors report a significant reduction in cell viability at 2 mT continuous exposure.…
The Dynamic(s) of Adipose Stem Cell System, Their Survival, and Cessation under the Influence of Electromagnetic Fields.
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RF Safe Research Library
Jan 1, 2021
This in vitro study examined how extremely low frequency EMF affects adipose-derived stem cell proliferation, cell cycle, viability, and death using fluorescent microscopy and flow cytometry. Under continuous exposure at 76.6 Hz and 20 T (ICR-based parameters), the authors report a statistically significant increase…
The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.
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RF Safe Research Library
Jan 1, 2021
This in vitro study exposed mouse spermatogonial stem cells to 50 Hz EMF (2.5 mT; 1 h/day for 5 days) and assessed oxidative stress and cell health outcomes. The authors report increased ROS and apoptosis with reduced antioxidant (catalase) expression, viability, and colonization after EMF exposure. Sertoli…
Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…
Nonionizing Electromagnetic Field: A Promising Alternative for Growing Control Yeast.
Research
RF Safe Research Library
Jan 1, 2021
This laboratory study examined radiofrequency EMF exposure between 1 and 5.9 GHz as a method to control yeast growth relevant to food spoilage. The authors report reduced yeast viability at all tested frequencies and note that antenna-to-sample distance affected the outcome. Electron microscopy findings are described…
Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure.
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RF Safe Research Library
Jan 1, 2021
This in vitro study exposed HaCaT human keratinocytes to 1760 MHz RF-EMF (SAR 4.0 W/kg; 2 h/day for 4 days). The authors report increased intracellular ROS and increased expression/activity of several matrix metalloproteinases, along with increased phosphorylation of FoxO3a and ERK1/2. Cell growth/viability and…
Effect of extremely low frequency electromagnetic field parameters on the proliferation of human breast cancer.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study tested how varying ELF-EMF parameters (frequency, flux density, duration, and continuous vs switching exposure) affect breast cancer and normal breast epithelial cell lines. The authors report that an optimized switching exposure pattern (7.83 ± 0.3 Hz, 1 mT) reduced viability of MDA-MB-231 cells…
Low-frequency electromagnetic field influences human oral mucosa keratinocyte viability in response to lipopolysaccharide or minocycline treatment in cell culture conditions.
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RF Safe Research Library
Jan 1, 2021
This in vitro study examined whether low-frequency electromagnetic field (LF-EMF) exposure affects viability of human oral mucosa keratinocytes. Apoptosis (early/late) and necrosis were measured by flow cytometry, including conditions with lipopolysaccharide (LPS) or minocycline. The authors report that LF-EMF, alone…
Effects of pulse-modulated radiofrequency magnetic field (RF-EMF) exposure on apoptosis, autophagy, oxidative stress and electron chain transport function in human neuroblastoma and murine microglial cells.
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RF Safe Research Library
Jan 1, 2020
This in vitro study exposed murine microglial (N9) and human neuroblastoma (SH-SY5Y) cells to ELF-modulated 935 MHz RF-EMF at 4 W/kg for 2 or 24 hours. The authors report no changes in apoptosis, cell viability, or AIF across conditions. They report increased ATG5 after 24 hours and a transient glutathione increase…
The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study tested whether 50 Hz extremely low-frequency electromagnetic fields (0.5 or 1 mT) affect human dental pulp stem cell survival and proliferation. Using MTT and DAPI assays after 7 days of exposure (20 or 40 min/day), all exposed groups reportedly showed higher viability/proliferation than controls.…
A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study examined effects of a 6 mT, 60 Hz uniform ELF electromagnetic field on HeLa cells and primary IMR-90 fibroblasts. Single or repetitive exposure did not induce DNA damage or change viability, but continuous exposure over 168 hours was reported to increase proliferation in both cell types in a…
Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal Cells.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study evaluated whether a therapeutic extremely low-frequency EMF exposure (5 Hz, 0.4 mT) causes cytotoxic or genotoxic effects in a human mesenchymal stromal cell line. Cells were exposed for 20 minutes per day, three times per week, for two weeks and assessed for viability, proliferation, and…
Extremely low frequency variable electromagnetic fields affect cancer and noncancerous cells in vitro differently: Preliminary study.
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RF Safe Research Library
Jan 1, 2018
This in vitro study exposed cancer (U87-MG; 143B) and noncancerous (BJ; HEK) cells to extremely low frequency electromagnetic fields under varying frequencies (2–60 Hz), waveforms, and exposure durations, with sham controls. Cell viability was assessed using an MTT assay. The authors report that cancer and…
RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study assessed whether intermittent 1800 MHz RF-EMF exposure induces DNA damage or alters cellular behavior in three neurogenic cell lines. Cells were exposed at an average SAR of 4.0 W/kg for 1, 6, or 24 hours and evaluated for γH2AX foci, cell cycle progression, proliferation, and viability. The…
Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study tested 50 Hz, 3 mT electromagnetic field (EMF) exposure and 808 nm low-level laser (LLL), alone and combined, on human adipose tissue-derived mesenchymal stem cells from six donors. The authors report increased cell numbers after 7 days with EMF, greater increases with LLL, and the largest…
Stable morphological-physiological and neural protein expression changes in rat bone marrow mesenchymal stem cells treated with electromagnetic field and nitric oxide.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study evaluated combined effects of extremely low frequency EMF (50 Hz, 20 mT) and nitric oxide (NO) on rat bone marrow mesenchymal stem cells. The authors report reduced viability and morphology changes with high NO in the presence of EMF, along with increased calcium ion entry. They also report…
Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study evaluated adult murine neural stem cells exposed to GSM 900-MHz radiofrequency emissions from a mobile phone across different exposure durations. The authors report significant decreases in neurosphere number/size and neuronal differentiation with increasing exposure duration. They report no…
Changes in viability of rat adipose-derived stem cells isolated from abdominal/perinuclear adipose tissue stimulated with pulsed electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study exposed rat adipose-derived stem cells to a pulsed electromagnetic field (7 Hz, 30 mT) and assessed viability by flow cytometry. The authors report that a low-fat diet was protective in PEMF-exposed ADSCs, especially in pups. They also report a higher proportion of early apoptotic cells in…
Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy.
Research
RF Safe Research Library
Jan 1, 2017
This study reports a technology that records a specific radiofrequency electromagnetic energy signal associated with EGFR siRNA and applies it to cells. The abstract describes reductions in EGFR mRNA and protein expression versus controls, with specificity assessed using RNA microarray and antibody dot blot array. It…
Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study exposed human microvascular endothelial cells to high-power microwave radiation at multiple power densities for 6 minutes and assessed cellular injury and cytoskeletal outcomes. The authors report dose-dependent injury from microwave exposure, including increased LDH leakage and reduced viability…
Gold Nanocluster-Mediated Cellular Death under Electromagnetic Radiation.
Research
RF Safe Research Library
Jan 1, 2017
This study investigated 2 nm protein-stabilized gold nanoclusters in six mammalian cell types under 1 GHz electromagnetic field exposure. The authors report low intrinsic toxicity of the nanoclusters, but a significant decrease in cell viability when nanocluster-incubated cells were exposed to the 1 GHz field,…
Association between mobile phone use and semen quality: a systemic review and meta-analysis
Research
RF Safe Research Library
Jan 1, 2014
This systematic review and meta-analysis assessed whether mobile phone use or radiofrequency exposure is associated with semen quality. Across included studies, the meta-analysis found no adverse effects in human studies, but reported detrimental effects on sperm motility and viability in vitro and harmful effects on…
Effect of mobile telephones on sperm quality: a systematic review and meta-analysis
Research
RF Safe Research Library
Jan 1, 2014
This 2014 systematic review and random-effects meta-analysis evaluated whether RF-EMR from mobile phones affects human sperm quality. Across 10 studies (1492 samples), exposure was associated with reduced sperm motility and reduced viability, while findings for sperm concentration were more equivocal. The abstract…