Stable morphological-physiological and neural protein expression changes in rat bone marrow mesenchymal stem cells treated with electromagnetic field and nitric oxide.
This in vitro study evaluated combined effects of extremely low frequency EMF (50 Hz, 20 mT) and nitric oxide (NO) on rat bone marrow mesenchymal stem cells. The authors report reduced viability and morphology changes with high NO in the presence of EMF, along with increased calcium ion entry. They also report neurite outgrowth and increased Map2 expression under high NO with EMF, which they interpret as a sign of neuronal differentiation progression.
Key points
- Rat BMSCs were exposed in culture to EMF (50 Hz, 20 mT) alongside NO donor (Deta-NO) and retinoic acid (RA).
- High NO in the presence of EMF was reported to decrease cell viability and change cell morphology.
- EMF exposure was reported to increase calcium ion entry into cells.
- RA-associated cell death and morphology changes were reported to intensify when combined with NO and EMF.
- Low NO concentration was reported to allow BMSCs to maintain a proliferative stem-cell state.
- High NO with EMF was associated with increased neurite number/length and a higher percentage of Map2-positive cells, interpreted as possible neuronal differentiation progression.
Referenced studies & papers
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AI-generated summaries may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.
AI-generated summaries may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.
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