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Protective effects of quercetin against 3.5 GHz RF radiation-induced thyroid dysfunction and oxidative stress in rats

Research RF Safe Research Library Jan 1, 2025

This rat study examined repeated 3.5 GHz RF exposure (2 hours/day, 5 days/week for 30 days) and thyroid-related outcomes, with and without quercetin. The abstract reports altered thyroid hormones (lower T3/T4, higher TSH) and increased oxidative stress in thyroid tissue after RF exposure. Quercetin appeared partially protective, though effects were not uniformly statistically significant, and SAR simulations indicated relatively higher absorption in the thyroid region.

The effects of short-term and long-term 2100 MHz radiofrequency radiation on adult rat auditory brainstem response

Research RF Safe Research Library Jan 1, 2025

This animal study examined 2100 MHz radiofrequency radiation exposure effects on auditory brainstem responses and brain oxidative/ultrastructural markers in adult rats. The 1-week exposure group showed prolonged ABR latencies and biochemical/structural changes consistent with oxidative stress and cellular injury. The authors report no harmful effects in the 10-week exposure condition with rest days under the studied protocol.

Characterization of the Core Temperature Response of Free-Moving Rats to 1.95 GHz Electromagnetic Fields

Research RF Safe Research Library Jan 1, 2025

This animal study measured core body temperature in free-moving male and female Sprague Dawley rats during and after 3-hour exposure to 1.95 GHz RF-EMF at multiple whole-body average SAR levels. A measurable thermal response was reported at 4 W/kg, while lower SAR conditions showed smaller or no significant temperature increases. The authors note that temperature dropped quickly after exposure ended, implying post-exposure measurements may underestimate peak heating.

The Influence of an Electromagnetic Field at a Radiofrequency of 900 MHz on the Behavior of a Honey Bee

Research RF Safe Research Library Jan 1, 2025

This laboratory study examined whether a 900 MHz radiofrequency electromagnetic field (RF-EMF) influences the behavior of newly emerged honey bee workers. Compared with controls, the exposed groups showed behavioral changes, with some parameters showing statistically significant differences seven days after exposure. The authors frame RF-EMF as a potential environmental stressor and call for further research, including gene expression analyses.

The influence of Wi-Fi on the mesonephros in the 9-day-old chicken embryo

Research RF Safe Research Library Jan 1, 2025

This animal study examined continual 2.4 GHz Wi‑Fi exposure (200–500 μW/m²) during 9 days of chicken embryo incubation and assessed the mesonephros at day 9. The authors report no adverse effects on general mesonephros development, but describe moderate degenerative changes and vascular congestion without inflammatory infiltrate. They also report significantly increased apoptotic and proliferating cells and up-regulation of caspase‑1 gene expression, interpreted as disruption of regulatory processes during development.

Single exposure to near-threshold 5G millimeter wave modifies restraint stress responses in rats

Research RF Safe Research Library Jan 1, 2025

In a rat experiment (n=59), a single 40-minute whole-body 28 GHz exposure at near-threshold WBA-SAR levels was evaluated under normal and heat conditions with restraint. After accounting for sham-related restraint stress, exposure was associated with increased serum-free corticosterone 1–3 days later, especially when rectal temperature rose by >1°C. Urinary catecholamines suggested an immediate inhibitory effect on stress response (notably noradrenaline), with heat amplifying effects and linking noradrenaline to tail surface temperature.

Effects of non-ionizing radiation on the thyroid gland in rats

Research RF Safe Research Library Jan 1, 2025

This animal study exposed Sprague-Dawley rats (including pregnant females and offspring) to 2.45 GHz Wi-Fi or mobile jammer radiation for 2 hours daily over two weeks and assessed thyroid hormones and thyroid histology. The abstract reports significant changes in T4 in exposed adult males and significant differences in T3 among male offspring exposed to jammer radiation. Histopathology reportedly showed disrupted thyroid follicular structure in exposed rats. The authors conclude these findings support a potential link between non-ionizing radiation exposure and altered thyroid endocrine and histological parameters.

An 1800 MHz Electromagnetic Field Affects Hormone Levels, Sperm Quality, and Behavior in Laboratory Rats

Research RF Safe Research Library Jan 1, 2025

This animal study exposed rats to a 1800 MHz electromagnetic field for 12 weeks and assessed hormones, sperm quality, and behavior. The abstract reports increased corticosterone, decreased thyroid-stimulating hormone, reduced sperm motility/viability, and increased anxiety-like behavior in exposed rats. Some hormonal changes reportedly persisted for at least 2 weeks after exposure ended, and the authors frame the results as indicating adverse endocrine, reproductive, and behavioral effects.

Impairment of Oogenesis and Folliculogenesis in Neonatal Rats after Maternal Exposure to Mobile Phones

Research RF Safe Research Library Jan 1, 2025

This animal study examined maternal mobile phone exposure during different gestational windows in Wistar rats and assessed ovarian development and hormones in neonatal offspring. Compared with sham (phone off), exposed groups (standby and conversation/standby) were reported to have lower neonatal estrogen and progesterone and reduced primordial follicle/primary oocyte measures, with stronger effects after longer exposure. The study also reports increased primordial follicle apoptosis, particularly in the conversation/standby condition, and notes effects even with first-week gestational exposure.

A Mini-Review of the Potential Health Impacts of Indoor Radiation Exposure in Companion Animals

Research RF Safe Research Library Jan 1, 2025

This mini-review discusses indoor radiation sources that may affect companion animals, including radon, radionuclides in feed, radiofrequency sources (phones, Wi-Fi, pet tracking devices), solar radiation, and extremely low frequency radiation. It reports that indoor radiation may negatively impact companion animal health and well-being. The authors conclude that preventive and precautionary measures are necessary to protect companion animals from indoor radiation exposure.

Melatonin ameliorates RF-EMR-induced reproductive damage by inhibiting ferroptosis through Nrf2 pathway activation

Research RF Safe Research Library Jan 1, 2025

This animal study reports that prolonged RF-EMR exposure (2.45 GHz for 8 weeks) increased oxidative stress and ferroptosis in mouse testicular tissue and was associated with reduced sperm quality. Melatonin administration reportedly mitigated oxidative injury and inhibited ferroptosis. The abstract attributes the protective effect to Nrf2 pathway activation via MT1/MT2 receptors.

Carcinogenicity of extremely low-frequency magnetic fields: A systematic review of animal studies

Research RF Safe Research Library Jan 1, 2025

This PRISMA-based systematic review evaluated 54 animal studies on the carcinogenicity of extremely low-frequency (ELF) magnetic fields. The authors report very little evidence that ELF magnetic fields alone are carcinogenic. Findings on co-carcinogenicity (ELF MFs combined with other agents) are inconclusive, and the review notes a clear indication of publication bias.

Effect of short-term extremely low-frequency electromagnetic field on respiratory functions

Research RF Safe Research Library Jan 1, 2025

This animal study tested whether short-term ELF-EMF exposure alters respiratory physiology in rats. Twenty Wistar albino rats were assigned to control or EMF exposure (50 Hz, 0.3 mT for 2 minutes) with respiratory parameters measured before, during, and after exposure. The study reports changes during exposure (lower respiratory rate and higher cycle duration, inspiration time, and tidal volume) but no differences after exposure, and it frames the findings as relevant to EMF safety and potential health risks.

Electromagnetic wireless remote control of mammalian transgene expression

Research RF Safe Research Library Jan 1, 2025

This animal proof-of-concept study describes an engineered nanoparticle–cell interface (EMPOWER) enabling wireless regulation of transgene expression using a 1-kHz magnetic field. Chitosan-coated multiferroic nanoparticles reportedly generate intracellular ROS that activates KEAP1/NRF2 biosensors connected to ROS-responsive promoters. In a mouse model of type 1 diabetes, implanted engineered cells expressing an EMPOWER-controlled insulin system reportedly normalized blood glucose in response to a weak magnetic field.

Effects of radiofrequency electromagnetic field exposure on cancer in laboratory animal studies, a systematic review

Research RF Safe Research Library Jan 1, 2025

This systematic review evaluated RF EMF exposure and cancer outcomes in experimental animals, including chronic cancer bioassays and tumor-promotion designs. Across 52 included studies, the authors report high certainty of evidence for increased malignant heart schwannomas and gliomas in male rats, and moderate certainty for increased risks of several other tumor types. Many other organ systems showed no or minimal evidence of carcinogenic effects, and the authors note challenges in translating animal findings to human risk assessment due to exposure and mechanistic uncertainties.

The effect on rat peripheral nerve morphology and function of a 900-MHz electromagnetic field applied in the prenatal period

Research RF Safe Research Library Jan 1, 2025

This animal study exposed pregnant Sprague Dawley rats to a 900 MHz electromagnetic field for 1 hour daily throughout gestation and assessed offspring outcomes postnatally. Sciatic nerve analyses at postnatal day 60 indicated persistent morphological alterations attributed to prenatal EMF exposure. However, the reported changes were not severe enough to significantly affect measured functional outcomes (including electrophysiology and locomotor tests).

Effects of 700MHz radiofrequency radiation (5G lower band) on the reproductive parameters of female Wistar rats

Research RF Safe Research Library Jan 1, 2025

This animal study examined short- and long-term 700 MHz (lower-band 5G) radiofrequency exposure in female Wistar rats, comparing control, sham, and exposed groups. It reports no DNA damage and no change in estrous cycle length, but increased ovarian oxidative stress markers in exposed animals. Long-term exposure was associated with ovarian histopathological alterations, while estradiol and progesterone stayed within normal ranges and testosterone increased slightly but significantly.

Possible effects of RF EMR on contextual fear conditioning, hippocampal perivascular space, apoptosis and adrenal gland microarchitecture in rats

Research RF Safe Research Library Jan 1, 2025

This animal study exposed male Wistar rats to 900 MHz RF-EMR from a mobile phone for 1 hour daily over four weeks and assessed behavior and tissue changes. The authors report altered contextual fear conditioning-related behavior in exposed rats. Histological assessments indicated apoptosis and enlarged perivascular space in the hippocampal CA3 region and apoptotic/inflammatory-like changes in the adrenal zona fasciculata, with no reported differences in adrenal medulla cytoarchitecture.

Repeated Head Exposures to a 5G-3.5 GHz Signal Do Not Alter Behavior but Modify Intracortical Gene Expression in Adult Male Mice

Research RF Safe Research Library Jan 1, 2025

This animal study examined repeated asymmetrical head exposure to a 5G-modulated 3.5 GHz signal in adult male mice for six weeks. It reports no significant changes in locomotion, anxiety, or object-based memory performance under the tested conditions. However, it found statistically significant but limited cortical gene expression changes (<1% of expressed genes), including enrichment for glutamatergic synapse-related genes and lateralized differences involving mitochondrial genome-encoded genes. The authors caution that potential health risks from these intracortical transcriptomic modifications should not be downplayed and note uncertainties about longer exposures and other populations.

5G Radiofrequency Exposure Reduces PRDM16 and C/EBP � mRNA Expression, Two Key Biomarkers for Brown Adipogenesis

Research RF Safe Research Library Jan 1, 2025

This animal study exposed juvenile and young adult Wistar rats to 5G (3.5 GHz) or 2G (900 MHz) radiofrequency fields (1.5 V/m) for 1–2 weeks and measured brown adipose tissue-related gene expression by RT-qPCR. The abstract reports significant downregulation of PRDM16 and C/EBP mRNA after 5G exposure, while UCP1-dependent thermogenesis markers were not significantly changed at the transcriptional level. The authors interpret these findings as a potential partial disruption of brown adipocyte differentiation and raise EMF safety concerns, while calling for further confirmatory research.

The CB1R of mPFC is involved in anxiety-like behavior induced by 0.8/2.65 GHz dual-frequency electromagnetic radiation

Research RF Safe Research Library Jan 1, 2025

This animal study reports that dual-frequency RF EMR exposure (0.8/2.65 GHz, 4 W/kg) induced anxiety-like behavior in mice. It also reports reduced CB1R and endocannabinoid levels in the mPFC and altered endocannabinoid system markers in the BLA. CB1R overexpression or knockdown in the mPFC reportedly decreased or increased anxiety-like behavior, respectively, suggesting a mechanistic link in this model.

Histomorphometry and Sperm Quality in Male Rats Exposed to 2.45 GHz Wi-Fi

Research RF Safe Research Library Jan 1, 2025

This animal study exposed adult male rats to 2.45 GHz Wi‑Fi from an active router for 4 or 24 hours daily over eight weeks and assessed reproductive organ histology and sperm parameters. The authors report histological changes in testes and epididymis, multifocal atypical hyperplasia in seminal vesicles, reduced seminiferous tubule diameter, and reduced spermatogenesis index in exposed groups. Sperm concentration decreased in both exposed groups, motility decreased in the 4-hour group, and viability increased in the 24-hour group, leading to an overall interpretation of potential reproductive risk under the studied conditions.

Extremely low frequency magnetic field distracts zebrafish from a visual cognitive task

Research RF Safe Research Library Jan 1, 2025

This animal study trained adult zebrafish to perform a conditioned avoidance response to a visual cue. The visual cue was presented alone or together with an extremely low frequency sinusoidally changing magnetic field (0.3 Hz) at 0.015 mT or 0.06 mT. The abstract reports that the 0.06 mT magnetic field condition impaired learning performance and response behavior, suggesting a cross-modal distraction effect.

Sensation of electric fields in the Drosophila melanogaster larva

Research RF Safe Research Library Jan 1, 2025

This animal study reports that Drosophila melanogaster larvae can sense electric fields and exhibit robust electrotaxis toward the cathode in controlled environments. The authors identify head-tip sensory neurons required for this behavior and report calcium-imaging evidence that Gr66a-positive neurons encode field strength and orientation. The work supports electrosensation as a functional sensory modality in Drosophila larvae and demonstrates measurable neural and behavioral responses to electric fields under the studied conditions.

The Impact of Mobile Phone Electromagnetic Waves on the Neurons and Blood Brain Barrier Integrity in the Chick Embryo

Research RF Safe Research Library Jan 1, 2024

This animal study exposed chick embryos to electromagnetic waves from a mobile phone and compared them with unexposed controls. Electron microscopy on days 10 and 15 reported neuronal and cerebellar cellular alterations in the exposed group, including features described as apoptosis and mitochondrial swelling. The authors also report compromised blood-brain barrier integrity and conclude the exposure adversely affects brain development.

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