[Fluorescence spectra analysis of papain treated by pulsed electric field].
This in vitro study used fluorescence excitation, emission, and polarization spectra to assess structural changes in papain after pulsed electric field (PEF) treatment. Under the reported PEF conditions (50 kV/cm, 1500 Hz, 40 µs pulses, 19,800 pulses), papain activity decreased by 56.5%. Spectral changes (increased excitation intensity, emission peak shift, reduced polarization) were interpreted as alpha-helix loosening/breakdown and exposure of fluorescent residues, potentially explaining enzyme inactivation.
Key points
- Papain contains intrinsic fluorescent amino acids enabling fluorescence-based structural assessment without external probes.
- PEF treatment was associated with a reported 56.5% reduction in papain relative activity under specified parameters.
- Fluorescence excitation intensity increased in treated samples, including at the 280 nm peak.
- The fluorescence emission peak shifted from 342 nm to approximately 346 nm after treatment.
- Fluorescence polarization degree was lower in treated samples than in untreated samples.
- Authors interpret the fluorescence changes as consistent with alpha-helix loosening/breakdown and residue exposure contributing to inactivation.
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AI-generated summaries may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.
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