Effects of Exposure to Radiofrequency at 2.45 GHz on Structural Changes Associated with Lipid Peroxidation in Prepubertal Rat Testicular Tissue

Abstract

Effects of Exposure to Radiofrequency at 2.45 GHz on Structural Changes Associated with Lipid Peroxidation in Prepubertal Rat Testicular Tissue Karadayı A, Ünal N, Gülbahçe ME Engiz B. Akkoca A, Varol S. (2023). Effects of Exposure to Radiofrequency at 2.45 GHz on Structural Changes Associated with Lipid Peroxidation in Prepubertal Rat Testicular Tissue. European Journal of Therapeutics. 2023. doi: 10.58600/eurjther1875. Abstract Objective: The increasing use of electronic devices, accompanied by advancing technologies, has led to heightened exposure to non-ionizing electromagnetic radiation (EMR). This exposure instigates the accumulation of free radicals and oxidative damage in tissues, consequently impacting biological systems. Notably, the testis is among the tissues adversely affected by EMR. Numerous studies have highlighted the pivotal role of the testis in sperm production, emphasizing the potential implications of any damage on the reproductive system. This study aims to assess the levels of lipid peroxidation through histological evaluation in the testicular tissue of prepubertal male rats exposed to electromagnetic radiation at varying electric field intensities within the 2.45 GHz radiofrequency (RF) range. Methods: The experimental group comprises six subdivisions, including a sham control group, as well as groups exposed to varying electric field strengths (EFS) of 0.6 V/m, 1.9 V/m, 5 V/m, 10 V/m, and 15 V/m, respectively. Excluding the sham control group, the remaining subgroups were subjected to a daily 2.45 GHz RF exposure for 1 hour starting immediately after fertilization. This exposure to different electric field intensities continued for 45 days post-birth. Results: The samples obtained from the RF radiation-exposed rats exhibited elevated malondialdehyde (MDA) values and decreased glutathione (GSH) values in the testicular tissue. Furthermore, a comparative analysis between the microwave radiation-exposed group and the control group revealed distinct histological alterations in the testicular tissue. Conclusion: In conclusion, our findings indicate that exposure to microwave radiation at an electric field intensity of 15 V/m can lead to significant histopathological and oxidative parameter changes in Wistar rats. These results underscore the potential effects of such exposure on human health. Open access paper: researchgate.net on_Structural_Changes_Associated_with_Lipid_Peroxidation_in_Prepubertal_Rat_Testicular_Tissue

AI evidence extraction

Main findings

Compared with sham control, RF-exposed rats showed increased MDA and decreased GSH in testicular tissue, along with distinct histological alterations. The authors conclude that exposure at 15 V/m produced significant histopathological and oxidative parameter changes.

Outcomes measured

• Testicular tissue malondialdehyde (MDA)
• Testicular tissue glutathione (GSH)
• Histological/histopathological changes in testicular tissue
• Lipid peroxidation/oxidative damage markers

Limitations

• Sample size not reported in abstract
• SAR/dosimetry details beyond electric field strength not reported in abstract
• Details of exposure setup and blinding/randomization not reported in abstract
• Results by each electric field strength level (0.6–15 V/m) not fully quantified in abstract

Suggested hubs

• rf-fertility-reproduction (0.9)
  Assesses testicular oxidative stress and histological changes after 2.45 GHz exposure in male rats.

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    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 2450,
        "sar_wkg": null,
        "duration": "1 hour/day; from immediately after fertilization through 45 days post-birth"
    },
    "population": "Prepubertal male Wistar rats (testicular tissue)",
    "sample_size": null,
    "outcomes": [
        "Testicular tissue malondialdehyde (MDA)",
        "Testicular tissue glutathione (GSH)",
        "Histological/histopathological changes in testicular tissue",
        "Lipid peroxidation/oxidative damage markers"
    ],
    "main_findings": "Compared with sham control, RF-exposed rats showed increased MDA and decreased GSH in testicular tissue, along with distinct histological alterations. The authors conclude that exposure at 15 V/m produced significant histopathological and oxidative parameter changes.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "SAR/dosimetry details beyond electric field strength not reported in abstract",
        "Details of exposure setup and blinding/randomization not reported in abstract",
        "Results by each electric field strength level (0.6–15 V/m) not fully quantified in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "unknown",
    "keywords": [
        "2.45 GHz",
        "radiofrequency",
        "microwave",
        "electric field strength",
        "Wistar rat",
        "prepubertal",
        "testis",
        "lipid peroxidation",
        "malondialdehyde",
        "glutathione",
        "oxidative stress",
        "histopathology"
    ],
    "suggested_hubs": [
        {
            "slug": "rf-fertility-reproduction",
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            "reason": "Assesses testicular oxidative stress and histological changes after 2.45 GHz exposure in male rats."
        }
    ]
}

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