Static electromagnetic field and recombinant human fibroblasts encoding miR-451 and miR-16 increased cell trans-differentiation to CD and CD erythroid like progenitor.

Abstract

INTRODUCTION: blood production is an urgent need of most countries, and creating production protocols can save the lives of many patients. Despite the recent advances in blood production in conditions, its high-scale production is not yet possible, and requires further studies. Therefore, by transfecting fibroblast cells with miR-16, and miR-451 genes, as well as applying low frequency electromagnetic fields (ELF-EMF) treatment, we tried to increase the differentiation of these cells into CD and CD erythroid like progenitors. METHODS: After preparation, and cultivation of human dermal transgenic fibroblast cells, they were transfected by Plenti3-hsa-miR451, Plenti3-hsa-miR16 and Plenti3-backbone inserted into Stbl4 genome. Then, transgenic fibroblast cells were treated with 10mT ELF-EMF every day for 20 minutes for 7 days. Using a flow cytometer, the expressions of CD, and CD were studied in these cells, and the expressions of genes involved in hematopoiesis were studied using the RT-PCR technique. RESULTS: The results indicated an increase in the differentiation of fibroblast cells treated with 10mT ELF-EMF to erythroid like progenitors. Furthermore, the percentage of CD and CD cells was the highest in irradiated cells encoding miR-16 and miR-451, which indicates their differentiation into erythroid like progenitors. Also, in the transgenic cells treated with ELF-EMF, an increase in the expressions of , , and genes was observed, which indicates the potential of these cells for hematopoiesis. However, there was no significant difference in the expression of and genes in these cell lines. CONCLUSION: Both ELF-EMF and upregulations of miR-16 and miR-451 lead to improved differentiation of fibroblast cells into erythroid like progenitors.

AI evidence extraction

Main findings

Human dermal transgenic fibroblast cells treated with 10 mT ELF-EMF for 20 minutes daily for 7 days showed increased differentiation toward erythroid-like progenitors. The highest percentage of CD and CD cells was reported in irradiated cells encoding miR-16 and miR-451, and several hematopoiesis-related genes increased with ELF-EMF treatment, while two genes showed no significant change.

Outcomes measured

• Differentiation/trans-differentiation of fibroblasts into erythroid-like progenitors
• Flow cytometry expression/percent of CD markers (CD and CD; specific numbers not provided)
• RT-PCR expression of hematopoiesis-related genes (some increased; two genes reported as not significantly different)

Limitations

• In vitro cell model; no human/animal outcomes reported
• EMF frequency not specified in the abstract
• Sample size and statistical details not provided
• Marker names and some gene names are not fully specified in the abstract (placeholders)

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "other",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "20 minutes/day for 7 days"
    },
    "population": "Human dermal transgenic fibroblast cells (recombinant; encoding miR-16 and/or miR-451)",
    "sample_size": null,
    "outcomes": [
        "Differentiation/trans-differentiation of fibroblasts into erythroid-like progenitors",
        "Flow cytometry expression/percent of CD markers (CD and CD; specific numbers not provided)",
        "RT-PCR expression of hematopoiesis-related genes (some increased; two genes reported as not significantly different)"
    ],
    "main_findings": "Human dermal transgenic fibroblast cells treated with 10 mT ELF-EMF for 20 minutes daily for 7 days showed increased differentiation toward erythroid-like progenitors. The highest percentage of CD and CD cells was reported in irradiated cells encoding miR-16 and miR-451, and several hematopoiesis-related genes increased with ELF-EMF treatment, while two genes showed no significant change.",
    "effect_direction": "benefit",
    "limitations": [
        "In vitro cell model; no human/animal outcomes reported",
        "EMF frequency not specified in the abstract",
        "Sample size and statistical details not provided",
        "Marker names and some gene names are not fully specified in the abstract (placeholders)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "low frequency electromagnetic fields",
        "10 mT",
        "fibroblasts",
        "transfection",
        "miR-16",
        "miR-451",
        "erythroid-like progenitors",
        "hematopoiesis",
        "flow cytometry",
        "RT-PCR"
    ],
    "suggested_hubs": []
}

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