Changes in miR-21, -138, -141, -135 expression and apoptosis/necrosis levels in HCT116 colon cancer cells under 50 Hz ELF-EMF exposure at 0.4 and 0.8 mT
Research
RF Safe Research Library
Feb 23, 2026
This in vitro study exposed HCT116 colorectal carcinoma cells to 50 Hz ELF-EMF at 0.4 or 0.8 mT for 9 or 18 hours under continuous or discontinuous conditions. The authors report significant changes in miR-21, miR-141, miR-135, and miR-138 expression and increased apoptosis and necrosis measured by flow cytometry.…
It is the Frequency that Matters: Effects of Electromagnetic Fields on the Release and Content of Extracellular Vesicles.
Research
RF Safe Research Library
Jan 1, 2023
This preprint describes an in vitro bioreactor-based system to test whether electromagnetic/electrical stimulation affects extracellular vesicles (EVs). Using proteomics and single-vesicle flow cytometry, the authors report that stimulation at physiological current amplitudes can modulate EV content in a…
The Dynamic(s) of Adipose Stem Cell System, Their Survival, and Cessation under the Influence of Electromagnetic Fields.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined how extremely low frequency EMF affects adipose-derived stem cell proliferation, cell cycle, viability, and death using fluorescent microscopy and flow cytometry. Under continuous exposure at 76.6 Hz and 20 T (ICR-based parameters), the authors report a statistically significant increase…
Low-frequency electromagnetic field influences human oral mucosa keratinocyte viability in response to lipopolysaccharide or minocycline treatment in cell culture conditions.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined whether low-frequency electromagnetic field (LF-EMF) exposure affects viability of human oral mucosa keratinocytes. Apoptosis (early/late) and necrosis were measured by flow cytometry, including conditions with lipopolysaccharide (LPS) or minocycline. The authors report that LF-EMF, alone…
Changes in viability of rat adipose-derived stem cells isolated from abdominal/perinuclear adipose tissue stimulated with pulsed electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study exposed rat adipose-derived stem cells to a pulsed electromagnetic field (7 Hz, 30 mT) and assessed viability by flow cytometry. The authors report that a low-fat diet was protective in PEMF-exposed ADSCs, especially in pups. They also report a higher proportion of early apoptotic cells in…
Aerodynamically assisted bio-jets: the development of a novel and direct non-electric field-driven methodology for engineering living organisms.
Research
RF Safe Research Library
Jan 1, 2007
This paper describes an aerodynamically assisted bio-jetting technique that processes living cells/organisms using differential pressure rather than an electric field. The authors report an operational window for stable jetting and near-monodispersed cell-bearing droplets. Cell viability after jetting was evaluated…