Cell surface changes during electromagnetic field exposure.

Abstract

Fibroblasts from 5 1/2-day-old chick embryos go through a sequential series of changes when exposed to a constant electromagnetic field (EMF) of 10 V/cm. These changes include rounding up, becoming bipolar in shape, assuming a cylindrical profile, elongating perpendicular to the EMF, and migrating to the cathode. These morphological changes are associated with changes of the cell surface, which include the formation of filopodia and extensive sheet-like contacts on the cathodic cell surface, the retraction of processes and the formation of focal contacts on the anodic cell surface.

AI evidence extraction

Main findings

Chick embryo fibroblasts exposed to a constant electromagnetic field of 10 V/cm underwent sequential morphological changes, including elongation perpendicular to the field and migration toward the cathode. These changes were associated with cell surface alterations, including filopodia and sheet-like contacts on the cathodic surface and retraction of processes with focal contacts on the anodic surface.

Outcomes measured

• Cell morphology changes (rounding, bipolar shape, cylindrical profile, elongation orientation)
• Cell migration direction (toward cathode)
• Cell surface changes (filopodia formation, sheet-like contacts, focal contacts, process retraction)

Limitations

• Frequency and other physical characteristics of the electromagnetic field are not specified beyond 10 V/cm.
• Exposure duration is not reported in the abstract.
• No sample size or quantitative/statistical results are provided in the abstract.
• In vitro chick embryo fibroblast model; generalizability to in vivo or human outcomes is not addressed in the abstract.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": null,
        "source": "other",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Fibroblasts from 5 1/2-day-old chick embryos",
    "sample_size": null,
    "outcomes": [
        "Cell morphology changes (rounding, bipolar shape, cylindrical profile, elongation orientation)",
        "Cell migration direction (toward cathode)",
        "Cell surface changes (filopodia formation, sheet-like contacts, focal contacts, process retraction)"
    ],
    "main_findings": "Chick embryo fibroblasts exposed to a constant electromagnetic field of 10 V/cm underwent sequential morphological changes, including elongation perpendicular to the field and migration toward the cathode. These changes were associated with cell surface alterations, including filopodia and sheet-like contacts on the cathodic surface and retraction of processes with focal contacts on the anodic surface.",
    "effect_direction": "unclear",
    "limitations": [
        "Frequency and other physical characteristics of the electromagnetic field are not specified beyond 10 V/cm.",
        "Exposure duration is not reported in the abstract.",
        "No sample size or quantitative/statistical results are provided in the abstract.",
        "In vitro chick embryo fibroblast model; generalizability to in vivo or human outcomes is not addressed in the abstract."
    ],
    "evidence_strength": "very_low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electromagnetic field",
        "10 V/cm",
        "chick embryo",
        "fibroblasts",
        "cell morphology",
        "cell migration",
        "cell surface",
        "filopodia",
        "focal contacts",
        "cathode",
        "anode"
    ],
    "suggested_hubs": []
}

Comments

Log in to comment.

No comments yet.