Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis

PAPER manual Iran J Cancer Prev 2014 In vitro study Effect: mixed Evidence: Low

Read original paper → PMID: 25250144 Evidence Lab

Abstract

Background: Extremely low frequency electromagnetic fields (ELF-EMF) have been common in daily life all over the world. They have produced by power lines and electrical appliances, but higher levels of them have raised a lot of concerns about their carcinogenesis. Both epidemiological and laboratory studies have suggested that EMFs might increase cancer incidence, including acute childhood leukemia, brain and breast cancer. Methods: In the present study, SH-SY5Y human neuroblastoma cell line has exposed to 2mT, 50 Hz magnetic field for 3 h. Next, effect of this exposure on protein expression including over-expression or under-expression has assessed by proteomics. Results: Bioinformatics and statistical analysis using progenesis same spot software on the obtained 2D electrophoresis has shown that expression of 189 proteins in exposed group has changed relative to control. Besides, PCA analysis has verified results of clustering, and has shown that protein data has clustered according to experimental conditions. Conclusion: The results of this study have shown that ELF-EMF changes cell morphology via altering protein expression, but more profound studies have needed to determine the kind of proteins altered.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

mixed

Population

SH-SY5Y human neuroblastoma cell line

Sample size

—

Exposure

ELF power lines and electrical appliances · 0.05 MHz · 3 h

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

SH-SY5Y cells exposed to a 2 mT, 50 Hz magnetic field for 3 hours showed changes in expression of 189 proteins relative to controls based on 2D electrophoresis analysis. PCA/clustering indicated protein data clustered by experimental condition, and the authors conclude ELF-EMF changes cell morphology via altered protein expression.

Outcomes measured

Protein expression changes (proteomics; 2D electrophoresis)
Cell morphology changes

Limitations

In vitro cell-line study (human neuroblastoma cells)
Sample size not reported in abstract
Specific proteins altered not determined/characterized in the abstract
Exposure limited to a single field strength (2 mT), frequency (50 Hz), and duration (3 h)

Suggested hubs

occupational-exposure (0.2)
Background mentions ELF-EMF from power lines/electrical appliances; no specific occupational setting studied.

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "power lines and electrical appliances",
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": "3 h"
    },
    "population": "SH-SY5Y human neuroblastoma cell line",
    "sample_size": null,
    "outcomes": [
        "Protein expression changes (proteomics; 2D electrophoresis)",
        "Cell morphology changes"
    ],
    "main_findings": "SH-SY5Y cells exposed to a 2 mT, 50 Hz magnetic field for 3 hours showed changes in expression of 189 proteins relative to controls based on 2D electrophoresis analysis. PCA/clustering indicated protein data clustered by experimental condition, and the authors conclude ELF-EMF changes cell morphology via altered protein expression.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro cell-line study (human neuroblastoma cells)",
        "Sample size not reported in abstract",
        "Specific proteins altered not determined/characterized in the abstract",
        "Exposure limited to a single field strength (2 mT), frequency (50 Hz), and duration (3 h)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "extremely low frequency",
        "50 Hz",
        "2 mT",
        "magnetic field",
        "SH-SY5Y",
        "human neuroblastoma",
        "proteomics",
        "2D electrophoresis",
        "PCA",
        "protein expression",
        "cell morphology"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.200000000000000011102230246251565404236316680908203125,
            "reason": "Background mentions ELF-EMF from power lines/electrical appliances; no specific occupational setting studied."
        }
    ]
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

Comments

No comments yet.