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Showing results for: protein expression

2026 EMF Research Snapshot: Non‑Thermal Biological Effects Across 6 GHz, 3.5 GHz, 2.45 GHz Wi‑Fi, and 28 GHz mmWave—Why Thermal‑Only Safety Limits Are Not Enough

Research Effect Synthesis Mar 1, 2026

Synthesis of 12 studies (2026) linking RF/EMF exposures and wireless tech use to oxidative stress, apoptosis, reproductive harm, kidney changes, sleep disruption, and base-station symptom patterns—supporting precautio…

High-Certainty Harm Evidence: RF/EMF Exposures Linked to Cancer, Reproductive Damage, and Pregnancy/Child Risks—Why Thermal-Only Safety Limits Fail

Research Effect Synthesis Mar 1, 2026

Synthesis of 17 high-evidence EMF/RF papers: systematic reviews and major bioassays report increased tumors in male rats, reduced male fertility (including lower pregnancy rates), and elevated risks for miscarriage an…

Proteomic Characterization of Human Peripheral Blood Mononuclear Cells Exposed to a 50 Hz Magnetic Field

Research RF Safe Research Library Jan 1, 2025

This in vitro study compared proteomic profiles of PBMCs from three human donors after 24-hour exposure to a 50 Hz, 1 mT extremely low-frequency magnetic field versus unexposed cells. The abstract reports broad protein expression changes, including upregulation of proteins associated with metabolic processes and…

Transcriptomic Analysis of Gene Expression and Effect of Electromagnetic Field in Brain Tissue after Traumatic Brain Injury.

Research RF Safe Research Library Jan 1, 2024

This pilot animal study used a Yucatan miniswine model of traumatic brain injury with and without EMF stimulation to examine differential gene expression in injured versus non-injured cortex tissue. The authors report multiple differentially expressed genes linked to immune infiltration, myelination, oxidative stress…

Alternating current electromagnetic field exposure lessens intramyocellular lipid accumulation due to high-fat feeding via enhanced lipid metabolism in mice.

Research RF Safe Research Library Jan 1, 2023

This mouse study tested whether alternating current electromagnetic field (EMF) exposure modifies muscle lipid accumulation during long-term high-fat feeding. Mice exposed to EMF (8 hours/day for 16 weeks; maximum 180 mT) while on a high-fat diet had lower intramyocellular lipid levels than high-fat diet controls.…

Protein expression changes during phagocytosis influenced by low-frequency electromagnetic field exposure.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined whether a low-frequency EMF (30 mT, 7 Hz for 3 h) alters phagocytosis of latex beads and immune-related protein/gene expression in the human Mono Mac 6 macrophage cell line. The authors report that EMF exposure influenced phagocytosis-associated molecular markers, with notable changes in…

Pulsed and Discontinuous Electromagnetic Field Exposure Decreases Temozolomide Resistance in Glioblastoma by Modulating the Expression of O-Methylguanine-DNA Methyltransferase, Cyclin-D1, and p53.

Research RF Safe Research Library Jan 1, 2021

This in vitro study evaluated 50 Hz, 70 G electromagnetic field exposure in combination with temozolomide (TMZ) in T98 and A172 glioblastoma cell lines. The authors report that EMF plus TMZ increased cell death and reduced migration potential compared with controls. They also report increased p53 gene expression and…

Extremely low frequency electromagnetic fields promote cognitive function and hippocampal neurogenesis of rats with cerebral ischemia.

Research RF Safe Research Library Jan 1, 2021

This animal study evaluated whether ELF-EMF exposure affects recovery-related outcomes after cerebral ischemia in rats. Rats exposed to 50 Hz, 1 mT ELF-EMF for 2 hours daily over 28 days showed better Morris water maze performance than untreated rats. The study also reports increased hippocampal neurogenesis markers…

Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression.

Research RF Safe Research Library Jan 1, 2019

This study assessed pre-sowing sunflower seed treatments using vacuum, 5.28 MHz radio-frequency cold plasma, and an electromagnetic field for varying durations. The authors report faster germination with cold plasma (7 min) and EMF (15 min), while germination percentage was mostly unchanged except for a decrease…

Stable morphological-physiological and neural protein expression changes in rat bone marrow mesenchymal stem cells treated with electromagnetic field and nitric oxide.

Research RF Safe Research Library Jan 1, 2017

This in vitro study evaluated combined effects of extremely low frequency EMF (50 Hz, 20 mT) and nitric oxide (NO) on rat bone marrow mesenchymal stem cells. The authors report reduced viability and morphology changes with high NO in the presence of EMF, along with increased calcium ion entry. They also report…

Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy.

Research RF Safe Research Library Jan 1, 2017

This study reports a technology that records a specific radiofrequency electromagnetic energy signal associated with EGFR siRNA and applies it to cells. The abstract describes reductions in EGFR mRNA and protein expression versus controls, with specificity assessed using RNA microarray and antibody dot blot array. It…

[Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation].

Research RF Safe Research Library Jan 1, 2016

This in vitro study exposed rat calvarial osteoblasts to 50 Hz, 0.6 mT pulsed electromagnetic fields for 1.5 hours and measured early differentiation-related markers over 0–12 hours. The abstract reports increased ALP activity at 3 hours and time-dependent changes in ALP, Runx2, and OSX transcription and Runx2/OSX…

Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis

Research RF Safe Research Library Jan 1, 2014

This in vitro study exposed SH-SY5Y human neuroblastoma cells to a 2 mT, 50 Hz magnetic field for 3 hours and assessed protein expression using proteomics. The authors report that 189 proteins changed expression in exposed cells compared with controls, with PCA/clustering separating samples by condition. They…

Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2013

This in vitro study examined whether extremely low frequency electromagnetic field (ELF-EMF) exposure alters MMP-2 expression in cultured human fetal scleral fibroblasts and whether puerarin modifies this response. The authors report increased MMP-2 mRNA and protein expression after 0.2 mT ELF-EMF exposure for 24…

Proteomic analysis in human fibroblasts by continuous exposure to extremely low-frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2007

This in vitro study examined whether continuous extremely low-frequency electromagnetic fields (3 Hz, 4 mT for 3 hours) alter protein expression in human fibroblast cells using proteomics. The authors report that some protein expressions differed between exposed and sham cells. They identify reduced expression (about…

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