Exposure to long-term evolution radiofrequency electromagnetic fields decreases neuroblastoma cell proliferation via Akt/mTOR-mediated cellular senescence.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined LTE RF-EMF exposure effects on SH-SY5Y neuroblastoma cells. The authors report reduced cell proliferation and a G0/G1 cell-cycle delay after 1760 MHz exposure at 4 W/kg for 4 hr/day over 4 days. They report no marked DNA damage signal (γH2AX) and no apoptosis activation, but increased…
Effects of pulse-modulated radiofrequency magnetic field (RF-EMF) exposure on apoptosis, autophagy, oxidative stress and electron chain transport function in human neuroblastoma and murine microglial cells.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study exposed murine microglial (N9) and human neuroblastoma (SH-SY5Y) cells to ELF-modulated 935 MHz RF-EMF at 4 W/kg for 2 or 24 hours. The authors report no changes in apoptosis, cell viability, or AIF across conditions. They report increased ATG5 after 24 hours and a transient glutathione increase…
Effects of electromagnetic field, cisplatin and morphine on cytotoxicity and expression levels of DNA repair genes.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study examined how morphine, a 50-Hz 0.50 mT electromagnetic field (intermittent), and cisplatin co-treatments affected DNA repair gene expression and cytotoxicity in MCF-7 and SH-SY5Y cells. Morphine alone was reported to down-regulate examined DNA repair genes, whereas morphine plus EMF did not…
Expressions of some antioxidant genes in SH-SY5Y cells treated with β-lapachone, morphine and electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed SH-SY5Y cells to a 50 Hz, 0.50 mT electromagnetic field under intermittent or continuous conditions, alongside β-lapachone and/or morphine. The authors report condition-dependent changes in antioxidant gene expression, including decreased NQO1 and increased NQO2 under intermittent EMF…
Electromagnetic Field Could Protect SH-SY5Y Cells Against Cisplatin Cytotoxicity, But Not MCF-7 Cells.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed MCF-7 and SH-SY5Y cells to a 50 Hz, 0.50 mT electromagnetic field in combination with cisplatin and/or morphine. The combined "CDDP+Mor+EMF" condition showed higher cisplatin IC values than cisplatin alone and "CDDP+Mor" in both cell lines, consistent with reduced cisplatin cytotoxicity.…
Effects of 1950 MHz radiofrequency electromagnetic fields on Aβ processing in human neuroblastoma and mouse hippocampal neuronal cells.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed HT22 mouse hippocampal neuronal cells and SH-SY5Y human neuroblastoma cells to 1950 MHz RF-EMF at SAR 6 W/kg for 2 hours per day over 3 days. RF-EMF exposure reduced mRNA levels of several Aβ-processing genes in HT22 cells, but did not change APP mRNA in SH-SY5Y cells. Protein levels of…
Different profiles of the mRNA levels of DNA repair genes in MCF-7 and SH-SY5Y cells after treatment with combination of cisplatin, 50-Hz electromagnetic field and bleomycin.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study examined whether a 50-Hz, 0.50 mT intermittent electromagnetic field modifies responses to cisplatin and bleomycin in MCF-7 and SH-SY5Y cells. The authors report increased susceptibility in MCF-7 cells with EMF added to cisplatin+bleomycin, while SH-SY5Y susceptibility was unchanged. They also…
Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis
Research
RF Safe Research Library
Jan 1, 2014
This in vitro study exposed SH-SY5Y human neuroblastoma cells to a 2 mT, 50 Hz magnetic field for 3 hours and assessed protein expression using proteomics. The authors report that 189 proteins changed expression in exposed cells compared with controls, with PCA/clustering separating samples by condition. They…