Proteomic Analysis of the Effect of ELF-EMF With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

Abstract

Proteomic Analysis of the Effect of ELF-EMF With Different Intensities in SH-SY5Y Neuroblastoma Cell Line Rezaie-Tavirani M, Hasanzadeh H, Seyyedi S, Zali H.Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line.J Lasers Med Sci. 2017 Spring;8(2):79-83. doi: 10.15171/jlms.2017.14. Abstract Introduction: During the last 3 decades, human is exposed to extremely low frequency electromagnetic fields (ELF-EMF) emitted by power lines and electronic devices. It is now well accepted that ELF-EMF are able to produce a variety of biological effects, although the molecular mechanism is unclear and controversial. Investigation of different intensities effects of 50 Hz ELF-EMF on cell morphology and protein expression is the aim of this study. Methods: SH-SY5Y human neuroblastoma cell line was exposed to 0.5 and 1 mT 50 Hz (ELF-EMF) for 3 hours. Proteomics techniques were used to determine the effects of these fields on protein expression. Bioinformatic and statistical analysis of proteomes were performed using Progensis SameSpots software. Results: Our results showed that exposure to ELF-EMF changes cell morphology and induces a dose-dependent decrease in the proliferation rate of the cells. The proteomic studies and bioinformatic analysis indicate that exposure to 50 Hz ELF-EMF leads to alteration of cell protein expression in both dose-dependent and intensity dependent manner, but the later is more pronounced. Conclusion: Our data suggests that increased intensity of ELF-EMF may be associated with more alteration in cell protein expression, as well as effect on cell morphology and proliferation. ncbi.nlm.nih.gov

AI evidence extraction

Main findings

SH-SY5Y cells exposed to 50 Hz ELF-EMF at 0.5 or 1 mT for 3 hours showed changes in cell morphology and a dose-dependent decrease in proliferation rate. Proteomic/bioinformatic analyses indicated altered protein expression with exposure, described as dose-dependent and intensity-dependent, with intensity effects reported as more pronounced.

Outcomes measured

• Cell morphology
• Cell proliferation rate
• Protein expression (proteomics)

Limitations

• In vitro cell line study; applicability to humans not addressed in abstract
• Sample size/replicates not reported in abstract
• Exposure duration limited to 3 hours

Suggested hubs

• occupational-exposure (0.25)
  Mentions ELF-EMF from power lines/electronic devices; general relevance to common/occupational ELF sources.

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        "duration": "3 hours"
    },
    "population": "SH-SY5Y human neuroblastoma cell line",
    "sample_size": null,
    "outcomes": [
        "Cell morphology",
        "Cell proliferation rate",
        "Protein expression (proteomics)"
    ],
    "main_findings": "SH-SY5Y cells exposed to 50 Hz ELF-EMF at 0.5 or 1 mT for 3 hours showed changes in cell morphology and a dose-dependent decrease in proliferation rate. Proteomic/bioinformatic analyses indicated altered protein expression with exposure, described as dose-dependent and intensity-dependent, with intensity effects reported as more pronounced.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cell line study; applicability to humans not addressed in abstract",
        "Sample size/replicates not reported in abstract",
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    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "50 Hz",
        "0.5 mT",
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        "proteomics",
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        "cell morphology",
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    "suggested_hubs": [
        {
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            "reason": "Mentions ELF-EMF from power lines/electronic devices; general relevance to common/occupational ELF sources."
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