EMF-induced mesenchymal stem cell migration is mediated by SOCE-dependent calcium influx and modulated by ROCK signaling.
Research
RF Safe Research Library
Jan 1, 2026
This in vitro study exposed rat bone marrow-derived mesenchymal stem cells to a 50 Hz, 5 mT sinusoidal EMF and assessed migration and signaling mechanisms. The authors report increased MSC migration that depended on extracellular Ca²⁺ influx and was abolished by SOCE inhibition, alongside increased Orai1 expression…
Extremely low frequency magnetic field distracts zebrafish from a visual cognitive task
Research
RF Safe Research Library
Jan 1, 2025
This animal study trained adult zebrafish to perform a conditioned avoidance response to a visual cue. The visual cue was presented alone or together with an extremely low frequency sinusoidally changing magnetic field (0.3 Hz) at 0.015 mT or 0.06 mT. The abstract reports that the 0.06 mT magnetic field condition…
Effect of ELF-EMF on cognitive functions, analgesia, and oxidative stress in rats with PTZ-induced epilepsy.
Research
RF Safe Research Library
Jan 1, 2025
This animal study tested repeated 50 Hz, 5 mT ELF-EMF exposure in rats with and without PTZ-induced epilepsy. The authors report changes in learning/short-term memory in the EMF+PTZ group versus PTZ alone and increased analgesia latency in EMF-exposed groups. PTZ increased oxidative stress (TOS) in brain regions, and…
Investigating the expression changes of several key genes in prostate cancer cells under exposure to the ELF pulsed electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2025
This in vitro study exposed DU-145 prostate cancer cells to extremely low-frequency pulsed electromagnetic fields (ELF P-EMF) at different intensities and times. The authors report increased cell mortality and apoptosis with higher intensity (22.6–35 mT) and longer exposure. They also report gene-expression changes…
Sinusoidal Extremely Low-Frequency Electromagnetic Stimulation (ELF-EMS) Promotes Angiogenesis In Vitro.
Research
RF Safe Research Library
Jan 1, 2025
This in vitro/in ovo study examined whether extremely low-frequency electromagnetic stimulation (ELF-EMS; 13.5 mT at 10 and 60 Hz) activates angiogenic processes under homeostatic conditions. In human microvascular endothelial cells, ELF-EMS increased proliferation, migration/transmigration, and tube formation, with…
Effects of Electromagnetic Field on Seed Germination, β-Amylase Activity, Total Protein Content, Water Uptake, and Growth of Wheat Seedlings (Triticum aestivum).
Research
RF Safe Research Library
Jan 1, 2025
This laboratory study examined whether extremely low frequency EMF exposure affects wheat seed germination and early seedling development. Seeds exposed to 10 Hz, 7.5 mT EMF (5 h over 3 days) reportedly germinated earlier and showed higher biomass than untreated controls. The abstract also reports increased total…
Electromagnetic field (50 Hz) enhance metabolic potential and induce adaptive/reprogramming response mediated by the increase of N6-methyladenosine RNA methylation in adipose-derived mesenchymal stem cells in vitro.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed adipose-derived mesenchymal stem cells to a 50 Hz electromagnetic field (1.5 mT) for 24 and 48 hours and assessed cellular responses. After 24 hours, the authors report increased N6-methyladenosine RNA methylation, increased expression of multiple stem cell markers, and changes in p21 and…
Extremely low-frequency electromagnetic field (ELF-EMF) induced alterations in gene expression and cytokine secretion in clear cell renal carcinoma cells.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed three ccRCC cell lines and a healthy kidney cell line (HEK293) to 50 Hz ELF-EMF at 4.5 mT for 30 minutes daily over 5 days. The authors report exposure-associated changes in expression of several genes (names not fully visible in the abstract), with differences between primary…
Extremely Low-Frequency Electromagnetic Stimulation (ELF-EMS) Improves Neurological Outcome and Reduces Microglial Reactivity in a Rodent Model of Global Transient Stroke.
Research
RF Safe Research Library
Jan 1, 2023
This animal and laboratory study evaluated extremely low-frequency electromagnetic stimulation (ELF-EMS) in a rodent ischemia-reperfusion stroke model and related in vitro/slice experiments. The authors report improved neurological and behavioral outcomes, increased neuronal survival, and reduced glial/microglial…
Optimization of cacao beans fermentation by native species and electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2023
This study optimized cacao bean fermentation for the CCN 51 clone using native microbial species and applied magnetic fields, varying field density, exposure time, and inoculum concentration. It reports improved yield and bean quality versus a no-magnetic-field control at an optimized condition (5 mT, 22.5 min, 1.6%…
Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined how low-frequency electromagnetic fields (LF-EMF) at different magnetic flux densities influence skeletal myogenesis in C2C12 cells. The authors report that 2.0 mT LF-EMF activated cells and increased PAX7, while 1.5 mT increased MyoD and myogenin expression. They conclude that different…
First Identification of the Effects of Low Frequency Electromagnetic Field on the Micromolecular Changes in Adipose Tissue-Derived Mesenchymal Stem Cells by Fourier Transform Infrared Spectroscopy.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed adipose tissue-derived mesenchymal stem cells to a continuous 50 Hz electromagnetic field at 1.5 mT for 24, 48, or 72 hours and assessed micromolecular changes using FTIR spectroscopy. The authors report the largest FTIR spectral differences versus control in nucleic acids and proteins…
The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed mouse spermatogonial stem cells to 50 Hz EMF (2.5 mT; 1 h/day for 5 days) and assessed oxidative stress and cell health outcomes. The authors report increased ROS and apoptosis with reduced antioxidant (catalase) expression, viability, and colonization after EMF exposure. Sertoli…
Effect of Rehabilitation with Extremely Low Frequency Electromagnetic Field on Molecular Mechanism of Apoptosis in Post-Stroke Patients.
Research
RF Safe Research Library
Jan 1, 2020
This study evaluated whether adding extremely low frequency electromagnetic field (ELF-EMF) exposure to a rehabilitation program affects apoptosis-related gene expression in post-stroke patients. Forty-eight patients (3–4 weeks post-incident) were divided into ELF-EMF and non-ELF-EMF groups, with the ELF-EMF group…
Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats.
Research
RF Safe Research Library
Jan 1, 2020
This rat study examined chronic extremely low-frequency electromagnetic field (ELF-EMF) exposure and diaphragm muscle function and structure. Rats were exposed to 50 Hz, 1.5 mT for 4 hours daily for up to 7 months and assessed with electrophysiological, biochemical, and electron-microscopy measures. The authors…
Effects of 5-HT and 5-HT Receptor Agonists on Electromagnetic Field-Induced Analgesia in Rats.
Research
RF Safe Research Library
Jan 1, 2019
This animal study examined whether serotonergic signaling modifies analgesia associated with repeated ELF electromagnetic field exposure. Sixty-six male Wistar rats were exposed to 50 Hz, 5 mT for 2 hours/day over 15 days and then received 5-HT agonists or antagonists before analgesia testing. Agonists increased,…
Effects of single and combined low frequency electromagnetic fields and simulated microgravity on gene expression of human mesenchymal stem cells during chondrogenesis.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human mesenchymal stem cell pellets undergoing chondrogenesis to simulated microgravity (SMG), low-frequency EMF (5 mT, 15 Hz), or both for 3 weeks. SMG significantly changed gene expression and reduced chondrogenic marker expression (including COL2A1). LF-EMF alone showed no…
Eotaxin‑1 and MCP‑1 serve as circulating indicators in response to power frequency electromagnetic field exposure in mice.
Research
RF Safe Research Library
Jan 1, 2018
This animal study exposed young Balb/c mice to constant 50 Hz power-frequency EMF at 0.1, 0.5, or 2.5 mT for 90 days. Body weight did not differ between exposed and control groups, but serum chemokine profiles showed exposure-related changes. EOTAXIN-1 and MCP-1 were significantly increased at 0.5 mT, which the…
Effects of extremely low frequency electromagnetic fields on morphine analgesia and tolerance in rats.
Research
RF Safe Research Library
Jan 1, 2017
This animal study examined whether extremely low frequency electromagnetic field exposure alters morphine analgesia and tolerance in rats. Rats exposed to a 50 Hz magnetic field (reported as 5 mT) showed higher morphine analgesic effects than rats given morphine without field exposure. In morphine-tolerant rats,…
Effect of electromagnetic fields on human osteoarthritic and non-osteoarthritic chondrocytes.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study exposed human osteoarthritic and non-osteoarthritic chondrocyte pellets to a sinusoidal 15 Hz electromagnetic field at 5 mT or 8 mT for 7 days. Under 5 mT, osteoarthritic chondrocytes showed increased expression of cartilage-related genes (COL2A1 and ACAN) compared with controls, while…
Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model.
Research
RF Safe Research Library
Jan 1, 2017
This animal study evaluated whether 50 Hz EMF exposure enhances wound healing after transplantation of human epidermal stem cells (ESC) seeded in collagen sponge scaffolds. Thirty-six nude mice with full-thickness wounds were assigned to control, ESC-only, or ESC+EMF groups (50 Hz, 5 mT, 60 min/day for 20 days). The…
P-selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2013
This animal study evaluated whether sinusoidal magnetic field exposure alters P-selectin expression in a rat colon tumor model. Rats exposed to 50 Hz, 5 mT for 6 h/day for 8 months showed no statistically significant change in P-selectin expression by immunohistochemistry. The authors indicate that further in vitro…
Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells.
Research
RF Safe Research Library
Jan 1, 2011
This in vitro study examined whether extremely low-frequency magnetic fields affect chondrogenic differentiation of human mesenchymal stem cells in pellet culture. Cells exposed for 3 weeks to a homogeneous sinusoidal 5 mT magnetic field showed increased collagen type II expression (significant at passage 6) and…