EMF-induced mesenchymal stem cell migration is mediated by SOCE-dependent calcium influx and modulated by ROCK signaling.
This in vitro study exposed rat bone marrow-derived mesenchymal stem cells to a 50 Hz, 5 mT sinusoidal EMF and assessed migration and signaling mechanisms. The authors report increased MSC migration that depended on extracellular Ca²⁺ influx and was abolished by SOCE inhibition, alongside increased Orai1 expression and SOCE activity. They also describe context-dependent modulation of ROCK1/2 and associated cytoskeletal remodeling, proposing SOCE as a key mediator of the observed EMF bioeffects.
Key points
- Rat MSCs exposed to 50 Hz, 5 mT EMF showed increased migration in Transwell assays.
- The pro-migratory effect was reported to require extracellular Ca²⁺ influx.
- SOCE inhibition with BTP2 abolished EMF-induced migration and reduced SOCE-related signaling readouts.
- EMF exposure was associated with upregulation of the SOCE channel Orai1 and increased SOCE activity.
- ROCK1/2 expression changes were described as biphasic and dependent on calcium conditions and SOCE status.
- SOCE inhibition attenuated EMF-associated cytoskeletal changes, including F-actin polymerization and perinuclear acetylated α-tubulin accumulation.
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AI-generated summaries may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.
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