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Showing results for: low-frequency

Modulation of inflammatory response by electromagnetic field in Neuronal and Microglial cells.

Research RF Safe Research Library Jan 1, 2025

This in vitro study examined whether low-frequency electromagnetic field (EMF) stimulation modulates inflammatory signaling in neuronal and microglial cell lines after induction of a pro-inflammatory state with TNF-α. The authors report reduced transcriptional expression of inflammatory target genes at 24 and 48…

Extremely low-frequency electromagnetic field (ELF-EMF) enhances mitochondrial energy production in NARP cybrids.

Research RF Safe Research Library Jan 1, 2025

This in vitro study examined ELF-EMF effects in normal cybrids and NARP cybrids carrying a pathogenic MT-ATP6 mtDNA mutation mixture. ELF-EMF did not alter mtDNA copy number or the wild-type-to-mutant mtDNA ratio, but it increased mtDNA transcription and multiple markers of mitochondrial function in NARP cybrids,…

Electromagnetic fields modulate neuronal membrane ionic currents through altered cellular calcium homeostasis.

Research RF Safe Research Library Jan 1, 2025

This experimental study used loose patch clamp recordings in coronal hippocampal slices to test effects of extremely low-frequency EMF exposure. A 50 Hz, 1 mT field applied for 60 minutes decreased inward and transient outward currents in CA1 pyramidal neuron membranes. Pharmacological manipulation of intracellular…

Sinusoidal Extremely Low-Frequency Electromagnetic Stimulation (ELF-EMS) Promotes Angiogenesis In Vitro.

Research RF Safe Research Library Jan 1, 2025

This in vitro/in ovo study examined whether extremely low-frequency electromagnetic stimulation (ELF-EMS; 13.5 mT at 10 and 60 Hz) activates angiogenic processes under homeostatic conditions. In human microvascular endothelial cells, ELF-EMS increased proliferation, migration/transmigration, and tube formation, with…

Growth and defense mechanism of Phaeocystis globosa exposed to extremely low frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2025

This laboratory study exposed cultured Phaeocystis globosa to extremely low-frequency electromagnetic fields (ELF-EMF) at 0, 0.2, and 1 mT for 24 hours. The authors report increased cell density, photosynthesis, and chlorophyll a under ELF-EMF, alongside decreased protein and carbohydrate accumulation. Metabolomic…

Performance Evaluation and Calibration of Electromagnetic Field (EMF) Area Monitors Using a Multi-Wire Transverse Electromagnetic (MWTEM) Transmission Line.

Research RF Safe Research Library Jan 1, 2025

This engineering study evaluates and calibrates an EMF area monitor for the 300 kHz–20 MHz frequency band using a multi-wire transverse electromagnetic (MWTEM) transmission line as a controlled electric-field source. The authors highlight that low-frequency electric-field measurements can be sensitive to boundary…

Effects of Extremely Low-Frequency Electromagnetic Field Treatment on ASD Symptoms in Children: A Pilot Study.

Research RF Safe Research Library Jan 1, 2024

This pilot study treated 20 children with ASD (ages 2–13) with a 15-week extremely low-frequency electromagnetic field (ELF-EMF) protocol using the SEQEX device. The abstract reports statistically significant improvements in receptive and expressive language and reductions in externalizing/behavioral problems, with…

The extremely low-frequency electromagnetic field (50 Hz) can establish a new "set-point" for the activity of the locus coeruleus-noradrenergic (LC-NA) system in rat.

Research RF Safe Research Library Jan 1, 2024

This animal study exposed rats to 50 Hz ELF-EMF at 1 mT or 7 mT for 1 hour/day over 7 days, repeated across three cycles, and assessed catecholamines, MHPG, β2-adrenergic receptor expression, and behavior. The authors report that ELF-EMF influences stress-response mechanisms involving the locus…

Effect of Electromagnetic Field on Proliferation and Migration of Fibroblasts and Keratinocytes: Implications in Wound Healing and Regeneration.

Research RF Safe Research Library Jan 1, 2024

This narrative review discusses reported effects of EMF, including low-frequency EMF (LF-EMF), on cell behaviors relevant to wound healing and on recovery processes after traumatic brain injury (TBI). It summarizes literature suggesting EMF can promote migration and proliferation of fibroblasts, keratinocytes, and…

Impact of Extremely Low-Frequency Electromagnetic Fields on Skeletal Muscle of Sedentary Adult Mice: A Pilot Study.

Research RF Safe Research Library Jan 1, 2024

This pilot animal study examined ELF-EMF exposure (0.1 or 1.0 mT, 1 h/day up to 5 weeks) in sedentary adult male C57BL/6 mice, with or without N-acetyl-cysteine (NAC) supplementation. The authors report a transient increase in muscle strength after 2 weeks at 1.0 mT, accompanied by higher PAX7 and myosin heavy chain…

Crosstalk between 6-methyladenine and 4-methylcytosine in exposed to extremely low-frequency electromagnetic field.

Research RF Safe Research Library Jan 1, 2024

This study examined prokaryotic DNA methylation (6mA and 4mC) and transcriptomes under different intensities of extremely low-frequency electromagnetic field (ELF-EMF) exposure. It reports motif-specific 6mA at 5'-GTACAG-3' and ELF-EMF-intensity-associated changes in spatial relationships among other 6mA sites,…

Thermodynamic Considerations on the Biophysical Interaction between Low-Energy Electromagnetic Fields and Biosystems.

Research RF Safe Research Library Jan 1, 2024

This paper presents a thermodynamic model intended to explain how extremely low-frequency electromagnetic fields (ELF-EMFs) may interact with cells. The authors propose that ELF-EMFs directly interfere with the cellular membrane, producing biological responses linked to energy conversion and changes in flows across…

Effect of prenatal exposure to stress and extremely low-frequency electromagnetic field on hippocampal and serum BDNF levels in male adult rat offspring.

Research RF Safe Research Library Jan 1, 2024

This rat study examined prenatal stress, prenatal ELF-EMF exposure, and their combination on adult male offspring outcomes at postnatal day 90. Prenatal stress increased serum corticosterone but did not change spatial memory, while combined stress+ELF-EMF disrupted memory acquisition. ELF-EMF exposure was associated…

The Extremely-Low-Frequency Electromagnetic Field Affects Apoptosis and Oxidative-Stress-Related Genes and Proteins in the Porcine Endometrium-An In Vitro Study.

Research RF Safe Research Library Jan 1, 2024

This in vitro study exposed porcine endometrial slices collected during the peri-implantation period to a 50 Hz extremely-low-frequency electromagnetic field for 2 hours. The authors report decreased CASP7 and increased NOS3/eNOS mRNA and protein abundance after exposure, along with increases in additional…

Chronic Drug-Resistant Cystitis Treated with a Sequence of Modulated Extremely Low-Frequency Electromagnetic Fields: A Randomized Study of 148 Cases.

Research RF Safe Research Library Jan 1, 2024

This randomized placebo-controlled study evaluated sequenced, modulated extremely low-frequency electromagnetic fields for drug-resistant chronic bacterial cystitis in 148 women. The treated group showed significant between-group differences at 4 weeks in multiple laboratory parameters, with reported sustained…

Extremely low-frequency electromagnetic field (ELF-EMF) induced alterations in gene expression and cytokine secretion in clear cell renal carcinoma cells.

Research RF Safe Research Library Jan 1, 2024

This in vitro study exposed three ccRCC cell lines and a healthy kidney cell line (HEK293) to 50 Hz ELF-EMF at 4.5 mT for 30 minutes daily over 5 days. The authors report exposure-associated changes in expression of several genes (names not fully visible in the abstract), with differences between primary…

Exposure to the extremely low-frequency electromagnetic field induces changes in the epigenetic regulation of gene expression in the endometrium.

Research RF Safe Research Library Jan 1, 2024

This in vitro study exposed porcine endometrial slices from days 15–16 of pregnancy to a 50 Hz extremely low-frequency electromagnetic field for 2 hours. The authors report changes in several epigenetic-related markers, including DNA methylation machinery, histone-related measures (HDAC activity), global DNA…

Effect of soil pH on thermally enhanced desorption of m-xylene by zero-valent iron particles under an electromagnetic field.

Research RF Safe Research Library Jan 1, 2023

This laboratory/engineering study evaluated thermally enhanced desorption of m-xylene from soil using heat generated by zero-valent iron (ZVI) under low-frequency electromagnetic fields. The abstract reports rapid heating (to ~60°C in 20 minutes) and substantially lower residual m-xylene after 60 minutes versus soil…

The Use of Induction Sensors and Helmet-Based Shielding Technology to Identify Differences in Electromagnetic Fields in Patients With Cranial Neurological Disease Versus Healthy Controls.

Research RF Safe Research Library Jan 1, 2023

This observational study used induction sensors with a Mu-metal and copper mesh shielded helmet to measure cortically generated EMFs in patients with cranial neurological disease and compare patterns to previously obtained healthy control data. Fourteen patients (ages 14–81) were analyzed and compared with four…

NET Formation Was Reduced via Exposure to Extremely Low-Frequency Pulsed Electromagnetic Fields.

Research RF Safe Research Library Jan 1, 2023

This in vitro study examined whether a 16 Hz extremely low-frequency pulsed electromagnetic field (ELF-PEMF) affects neutrophil extracellular trap (NET) formation. In neutrophils from healthy volunteers, ELF-PEMF exposure did not induce ROS or calcium influx and did not increase basal or PMA-induced NET formation.…

The Effect of Low-Frequency Pulsed Electromagnetic Fields on the Differentiation of Permanent Dental Pulp Stem Cells into Odontoblasts.

Research RF Safe Research Library Jan 1, 2023

This in vitro study exposed dental pulp mesenchymal stem cells to 50 Hz pulsed electromagnetic fields at 0.5 or 1 mT for 20 or 40 minutes per day over seven days. Cell survival/proliferation was generally higher than control across exposure groups, except for the 40 minutes/day at 1 mT condition. DMP1 expression was…

Human osteosarcoma cells in response to ELF-MF: Morphological remodeling compared to cell proliferation.

Research RF Safe Research Library Jan 1, 2023

This in vitro study exposed human osteosarcoma (MG-63) cells to 2 mT extremely low-frequency magnetic fields with sinusoidal, triangular, or pulsed waveforms under continuous (3 h) or fractionated (3 days) conditions. The authors report significant decreases in proliferation and shape index in exposed cells versus…

A Swine Model of Neural Circuit Electromagnetic Fields: Effects of Immediate Electromagnetic Field Stimulation on Cortical Injury.

Research RF Safe Research Library Jan 1, 2023

This animal study used non-contact induction sensors and a shielded helmet to measure cortical EMF patterns in a Yucatan miniswine before and after traumatic brain injury induced by controlled cortical impact. The authors report that targeted low-frequency EMF stimulation initiated 20 minutes post-injury was…

The Effect of Extremely Low-Frequency Electromagnetic Fields on Inflammation and Performance-Related Indices in Trained Athletes: A Double-Blinded Crossover Study.

Research RF Safe Research Library Jan 1, 2023

This double-blinded crossover study tested an ELF-EMF mattress used immediately after high-intensity cycling and overnight in nine trained male athletes. The authors report no significant overall effects on performance and inflammation. However, between 60 minutes and 24 hours post-exercise, the active condition…

A Swine Model of Changes in the Neuronal Electromagnetic Field After Traumatic Brain Injury: A Pilot Study.

Research RF Safe Research Library Jan 1, 2023

This pilot swine study evaluated whether non-contact proprietary induction sensors in a Mu-metal/copper mesh helmet can measure endogenous neuronal EMF signals before and after controlled cortical impact traumatic brain injury. The authors report that swine EMF recordings were distinguishable from room noise and…

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