Dose-dependent impact of extremely low-frequency electromagnetic field (ELF-EMF) on the neuroplasticity in the hippocampus of adult rats.
Research
RF Safe Research Library
Jan 1, 2026
This animal study examined whether 50 Hz ELF-EMF exposure at two intensities (1 mT vs 7 mT) alters hippocampal gene transcripts related to neuroplasticity and cell survival in adult rats across repeated exposure cycles. The authors report intensity- and exposure-number-dependent modulation of mRNA expression, with 1…
Radiofrequency radiation-induced gene expression
Research
RF Safe Research Library
Jan 1, 2025
This review summarizes studies reporting radiofrequency radiation (RFR)-associated changes in gene expression across biological systems. Reported affected genes relate to cellular stress responses, oxidative processes, apoptosis, DNA damage detection/repair, protein repair, and neural function regulation. The authors…
Radiofrequency regulates the BET-mediated pathways in radial glia differentiation in human cortical development
Research
RF Safe Research Library
Jan 1, 2025
This in vitro study reports that radiofrequency (RF) exposure in the 800–2,400 MHz range modulates differentiation pathways in human cortical organoids derived from embryonic stem cells. RF exposure is described as maintaining radial glia stem cell identity and delaying differentiation, alongside induction of…
5G-exposed human skin cells do not respond with altered gene expression and methylation profiles
Research
RF Safe Research Library
Jan 1, 2025
This in vitro study exposed human skin cells (fibroblasts and keratinocytes) to 5G-band electromagnetic fields for 2 hours and 48 hours using a fully blinded design. Exposures were up to ten times permissible limits, with sham exposure as a negative control and UV exposure as a positive control. The study reports…
Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period
Research
RF Safe Research Library
Jan 1, 2025
This in vitro study exposed pig conceptuses (days 15–16 of pregnancy) to 50 Hz ELF-EMF for 2 hours and assessed transcriptomic and DNA methylation changes. The authors report altered expression of 21 protein-coding transcripts and an approximately 16-fold increase in genomic DNA methylation, with promoter methylation…
Repeated Head Exposures to a 5G-3.5 GHz Signal Do Not Alter Behavior but Modify Intracortical Gene Expression in Adult Male Mice
Research
RF Safe Research Library
Jan 1, 2025
This animal study examined repeated asymmetrical head exposure to a 5G-modulated 3.5 GHz signal in adult male mice for six weeks. It reports no significant changes in locomotion, anxiety, or object-based memory performance under the tested conditions. However, it found statistically significant but limited cortical…
5G Radiofrequency Exposure Reduces PRDM16 and C/EBP � mRNA Expression, Two Key Biomarkers for Brown Adipogenesis
Research
RF Safe Research Library
Jan 1, 2025
This animal study exposed juvenile and young adult Wistar rats to 5G (3.5 GHz) or 2G (900 MHz) radiofrequency fields (1.5 V/m) for 1–2 weeks and measured brown adipose tissue-related gene expression by RT-qPCR. The abstract reports significant downregulation of PRDM16 and C/EBP mRNA after 5G exposure, while…
Electric and Magnetic Field Technologies in Agriculture: Plant Responses, Experimental Limitations, and Future Directions
Research
RF Safe Research Library
Jan 1, 2024
This narrative review discusses how electric and magnetic field technologies are applied in agriculture and how plants may respond. It proposes a model to explain potential mechanistic convergence between electric and magnetic field effects. The review highlights several suggested mechanisms, including membrane…
Investigating the effect of radiofrequency electromagnetic field exposure on molecular pathways related to insulin resistance and adipogenesis in zebrafish embryos - A pilot study without quantitative exposure metrics.
Research
RF Safe Research Library
Jan 1, 2024
This pilot animal study exposed zebrafish embryos to 900 MHz RF-EMF for 30 or 60 minutes per day until 96 hours post-fertilization and assessed gene expression and physiological endpoints. The authors report reduced expression of lepa, ins, and pparg, changes in oxidative stress markers consistent with increased…
Anthropogenic electromagnetic radiation alters the transcription levels of the genes encoding the SIFamide and myoinhibitory peptide and their receptors in Ixodes ricinus synganglion.
Research
RF Safe Research Library
Jan 1, 2024
This animal study used qRT-PCR to assess whether 900 MHz radiofrequency electromagnetic field exposure alters neuropeptide-related gene transcription in Ixodes ricinus synganglia. The abstract reports that 40 V/m exposure for at least 60 minutes significantly suppressed transcript levels of sifa, mip, and their…
The Extremely-Low-Frequency Electromagnetic Field Affects Apoptosis and Oxidative-Stress-Related Genes and Proteins in the Porcine Endometrium-An In Vitro Study.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed porcine endometrial slices collected during the peri-implantation period to a 50 Hz extremely-low-frequency electromagnetic field for 2 hours. The authors report decreased CASP7 and increased NOS3/eNOS mRNA and protein abundance after exposure, along with increases in additional…
Extremely low-frequency electromagnetic field (ELF-EMF) induced alterations in gene expression and cytokine secretion in clear cell renal carcinoma cells.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed three ccRCC cell lines and a healthy kidney cell line (HEK293) to 50 Hz ELF-EMF at 4.5 mT for 30 minutes daily over 5 days. The authors report exposure-associated changes in expression of several genes (names not fully visible in the abstract), with differences between primary…
Transcriptome profiling of flax plants exposed to a low-frequency alternating electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2023
This study aims to perform transcriptome profiling in flax plants exposed to a low-frequency alternating electromagnetic field. The abstract frames prior work as suggesting EMF-related changes in free radical homeostasis and altered expression of genes involved in stress responses, ion transport, and cell wall…
Intermittent Exposure to a 16 Hz Extremely Low Frequency Pulsed Electromagnetic Field Promotes Osteogenesis In Vitro through Activating Piezo 1-Induced Ca Influx in Osteoprogenitor Cells.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study compared continuous versus intermittent 16 Hz ELF-PEMF exposure strategies in osteoprogenitor (SCP-1) cells. Intermittent exposure (10 min every 8 h) was reported to enhance cell numbers and osteogenic function relative to continuous daily exposure (30 min every 24 h). The authors report increased…
Effect of extremely low frequency electromagnetic field on the pathogenicity of Magnaporthe oryzae.
Research
RF Safe Research Library
Jan 1, 2023
This laboratory study examined Magnaporthe oryzae pathogenicity under extremely low frequency electromagnetic field exposure (50 Hz, 3 mT). The authors report increased infection ability, growth rate, and conidia generation with ELF-EMF exposure. They also report increased tolerance to a cell wall-perturbing agent…
Comparative Study of Extremely Low-Frequency Electromagnetic Field, Radiation, and Temozolomide Administration in Spheroid and Monolayer Forms of the Glioblastoma Cell Line (T98).
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study assessed 50 Hz, 100 G extremely low-frequency EMF in combination with temozolomide and radiation in T98 glioblastoma cells grown as monolayers and spheroids. Radiation plus EMF increased apoptosis-related gene expression, while temozolomide plus EMF increased autophagy marker expression compared…
Enhancement of Fungal Enzyme Production by Radio-Frequency Electromagnetic Fields.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined whether a 2 GHz RF-EMF exposure could enhance enzyme production in a filamentous fungus. A 10-minute exposure was reported to increase extracellular total protein and α-amylase activity in culture media versus controls, alongside increased α-amylase mRNA and changes consistent with…
Cardiac Cell Exposure to Electromagnetic Fields: Focus on Oxdative Stress and Apoptosis.
Research
RF Safe Research Library
Jan 1, 2022
This study assessed whether 915 MHz RF-EMF exposure alters oxidative stress and apoptosis markers in cardiac cells and mouse heart tissue. The authors report no statistically significant differences in apoptotic profile, antioxidant capacity, tissue integrity, or expression of apoptosis/antioxidant genes after…
Evaluation of Cell Migration and Cytokines Expression Changes under the Radiofrequency Electromagnetic Field on Wound Healing In Vitro Model.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study evaluated whether radiofrequency electromagnetic field (RF-EMF) exposure could influence wound-healing-related processes. The authors report increased keratinocyte migration and changes in expression of wound-healing genes, including MMPs, TIMPs, and pro/anti-inflammatory cytokines. The abstract…
Anticancer and antibacterial potentials induced post short-term exposure to electromagnetic field and silver nanoparticles and related pathological and genetic alterations: in vitro study.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study evaluated short-term extremely low frequency electromagnetic field (ELF-EMF) exposure and silver nanoparticles (AgNPs), alone and combined, for antibacterial effects and apoptosis induction in MCF-7 breast cancer cells. The authors report time-dependent reductions in bacterial viable counts, with…
Electromagnetic Field (EMF) Radiation Alters Estrogen Release from the Pig Myometrium during the Peri-Implantation Period.
Research
RF Safe Research Library
Jan 1, 2021
This ex vivo animal-tissue study tested whether ELF-EMF exposure (50 or 120 Hz for 2 or 4 hours) alters estrogenic activity in pig myometrium during the peri-implantation period. The authors report EMF-associated changes in estrogen-related gene transcripts and aromatase abundance, with progesterone diminishing some…
Enhancement in the ATP level and antioxidant capacity of under continuous exposure to extremely low-frequency electromagnetic field for multiple generations.
Research
RF Safe Research Library
Jan 1, 2020
This multi-generation animal study exposed worms continuously to 50 Hz, 3 mT extremely low-frequency electromagnetic fields and assessed outcomes in the 15th generation. The study reports increased body length, higher ATP content and ATP synthase activity, and upregulated ATP-related gene expression in exposed worms.…
Effect of Rehabilitation with Extremely Low Frequency Electromagnetic Field on Molecular Mechanism of Apoptosis in Post-Stroke Patients.
Research
RF Safe Research Library
Jan 1, 2020
This study evaluated whether adding extremely low frequency electromagnetic field (ELF-EMF) exposure to a rehabilitation program affects apoptosis-related gene expression in post-stroke patients. Forty-eight patients (3–4 weeks post-incident) were divided into ELF-EMF and non-ELF-EMF groups, with the ELF-EMF group…
Evaluation of the effects of extremely low frequency electromagnetic field on the levels of some inflammatory cytokines in post-stroke patients.
Research
RF Safe Research Library
Jan 1, 2019
This study evaluated whether adding therapeutic extremely low frequency electromagnetic field (ELF-EMF) treatment to a rehabilitation program affects inflammatory cytokine activity in post-stroke patients. Following ELF-EMF treatment, IL-1β plasma levels and IL-1β mRNA expression increased, and IL-2 plasma levels…
Efficacy of pulsed electromagnetic fields and electromagnetic fields tuned to the ion cyclotron resonance frequency of Ca on chondrogenic differentiation.
Research
RF Safe Research Library
Jan 1, 2019
This in vitro study compared commonly used pulsed electromagnetic field (PEMF) exposures with electromagnetic fields tuned to satisfy the ion cyclotron resonance (ICR) condition for calcium. After 3 weeks, the calcium ICR condition was associated with increased glycosaminoglycans and type II collagen synthesis and…