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P53 status, and G2/M cell cycle arrest, are determining factors in cell-death induction mediated by ELF-EMF in glioblastoma.

Research RF Safe Research Library Jan 1, 2023

This in vitro study tested ELF-EMF exposure in U87 and U251 glioblastoma cell lines and assessed apoptosis, cell-cycle distribution, cell size, and mRNA expression of cell-cycle genes. The authors report higher apoptosis induction and increased G2/M arrest in U87 compared with U251, alongside increased U87 cell size…

Synergistic cytotoxic effects of an extremely low-frequency electromagnetic field with doxorubicin on MCF-7 cell line.

Research RF Safe Research Library Jan 1, 2023

This in vitro study tested whether a 50 Hz, 20 mT extremely low-frequency electromagnetic field (ELF-EMF) enhances the cytotoxic effects of doxorubicin (DOX) in the MCF-7 breast cancer cell line. The authors report that combining ELF-EMF with low-dose DOX reduced cell viability at lower DOX concentrations than DOX…

Electromagnetic fields ameliorate hepatic lipid accumulation and oxidative stress: potential role of CaMKKβ/AMPK/SREBP-1c and Nrf2 pathways.

Research RF Safe Research Library Jan 1, 2023

This animal study examined EMF exposure in a mouse high-fat diet model of NAFLD. The authors report that EMF exposure reduced hepatic lipid accumulation and markers of oxidative stress, alongside changes consistent with activation of CaMKKβ/AMPK and Nrf2-related signaling. The abstract concludes EMF could be a…

Electromagnetic Fields Literature Analysis for Precision Medicine.

Research RF Safe Research Library Jan 1, 2023

This paper describes a literature-mining analysis of more than 30,000 EMF-related publications to extract genes, diseases, and molecular mechanisms linked to EMF exposure across six EMF subsets. The authors report identifying 3,653 unique disease MeSH terms and 9,966 unique genes, including 4,340 human genes. The…

Intermittent Exposure to a 16 Hz Extremely Low Frequency Pulsed Electromagnetic Field Promotes Osteogenesis In Vitro through Activating Piezo 1-Induced Ca Influx in Osteoprogenitor Cells.

Research RF Safe Research Library Jan 1, 2023

This in vitro study compared continuous versus intermittent 16 Hz ELF-PEMF exposure strategies in osteoprogenitor (SCP-1) cells. Intermittent exposure (10 min every 8 h) was reported to enhance cell numbers and osteogenic function relative to continuous daily exposure (30 min every 24 h). The authors report increased…

Immunomodulatory Effect of Electromagnetic Field in the Treatment of Traumatic Brain Injury.

Research RF Safe Research Library Jan 1, 2023

This paper reports a PubMed and Google Scholar-based review of EMF research related to brain pathology, with emphasis on ischemic and traumatic brain injury. It provides a critical overview of the state of the art and describes the authors’ experimental and ongoing studies. The authors conclude that EMF applications…

Magnetofection of miR-21 promoted by electromagnetic field and iron oxide nanoparticles via the p38 MAPK pathway contributes to osteogenesis and angiogenesis for intervertebral fusion.

Research RF Safe Research Library Jan 1, 2023

This study describes a magnetofection system using electromagnetic field (EMF) co-stimulation with iron oxide nanoparticles (IONPs) to deliver miR-21. The authors report increased transfection efficiency and promotion of osteogenesis and angiogenesis in BMSCs and HUVECs, with downstream application to…

Low-energy amplitude-modulated radiofrequency electromagnetic fields as a systemic treatment for cancer: Review and proposed mechanisms of action

Research RF Safe Research Library Sep 8, 2022

This review discusses low-energy amplitude-modulated RF EMF (27.12 MHz carrier with low Hz–kHz envelope modulation) delivered systemically via intrabuccal antenna devices as a potential treatment for advanced hepatocellular carcinoma. It reports preclinical inhibition of cancer growth in vitro and in mouse xenograft…

Mg.ATP-decorated ultrafine magnetic nanofibers: A bone scaffold with high osteogenic and antibacterial properties in the presence of an electromagnetic field.

Research RF Safe Research Library Jan 1, 2022

This in vitro study developed magnetic electrospun nanofibers for bone regeneration and evaluated osteogenic and antibacterial properties. Functionalization with ATP and Mg increased mesenchymal stem cell adhesion, proliferation, and osteogenic marker readouts compared with unmodified fibers. The authors report that…

Consciousness and inward electromagnetic field interactions.

Research RF Safe Research Library Jan 1, 2022

This review discusses longstanding EMF theories of mind/brain integration as a potential explanation for unified conscious experience and aspects of the "hard problem" of consciousness. It notes that EMFs are measurable and that correlates have been reported with changes in consciousness, sensory perception, and…

Enhancement of Fungal Enzyme Production by Radio-Frequency Electromagnetic Fields.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined whether a 2 GHz RF-EMF exposure could enhance enzyme production in a filamentous fungus. A 10-minute exposure was reported to increase extracellular total protein and α-amylase activity in culture media versus controls, alongside increased α-amylase mRNA and changes consistent with…

PIXE analysis of iron in rabbit cerebellum after exposure to radiofrequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2022

This animal study assessed iron in rabbit cerebellum after exposure to real GSM and generated RF electromagnetic fields using ICP MS and PIXE. ICP MS found no significant differences in overall iron concentration between exposed and control groups. PIXE reported focal iron accumulation up to about 3 mm, with the…

High-specificity protection against radiation-induced bone loss by a pulsed electromagnetic field.

Research RF Safe Research Library Jan 1, 2022

This study reports a noninvasive pulsed-burst electromagnetic field (PEMF) approach intended to protect against radiotherapy-induced bone loss. A 15 Hz, 2 mT PEMF waveform is described as producing distinctive calcium oscillations in osteoblasts and inhibiting radiation-related bone loss while not affecting other…

Influence of Schumann Range Electromagnetic Fields on Components of Plant Redox Metabolism in Wheat and Peas.

Research RF Safe Research Library Jan 1, 2022

This study examined whether Schumann Resonance-range ELF electromagnetic fields (7.8, 14.3, 20.8 Hz) influence redox metabolism components in wheat and pea leaves after short (30 min) and long (18 days) exposures. The authors report that short-time exposure produced more pronounced biological effects than long-time…

Protein expression changes during phagocytosis influenced by low-frequency electromagnetic field exposure.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined whether a low-frequency EMF (30 mT, 7 Hz for 3 h) alters phagocytosis of latex beads and immune-related protein/gene expression in the human Mono Mac 6 macrophage cell line. The authors report that EMF exposure influenced phagocytosis-associated molecular markers, with notable changes in…

Extremely Low-Frequency Electromagnetic Fields Increase Cytokines in Human Hair Follicles through Wnt/β-Catenin Signaling.

Research RF Safe Research Library Jan 1, 2022

This in vitro/ex vivo study examined extremely low-frequency EMF exposure in human hair bulb spheroids and hair follicle organ cultures. The authors report no toxicity and increased expression of anagen-related markers and multiple cytokines/growth factors at 5–20 G, with 10 G for 60 minutes associated with increased…

Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined how low-frequency electromagnetic fields (LF-EMF) at different magnetic flux densities influence skeletal myogenesis in C2C12 cells. The authors report that 2.0 mT LF-EMF activated cells and increased PAX7, while 1.5 mT increased MyoD and myogenin expression. They conclude that different…

Anticancer and antibacterial potentials induced post short-term exposure to electromagnetic field and silver nanoparticles and related pathological and genetic alterations: in vitro study.

Research RF Safe Research Library Jan 1, 2022

This in vitro study evaluated short-term extremely low frequency electromagnetic field (ELF-EMF) exposure and silver nanoparticles (AgNPs), alone and combined, for antibacterial effects and apoptosis induction in MCF-7 breast cancer cells. The authors report time-dependent reductions in bacterial viable counts, with…

Cancer on-target: Selective enhancement of 3-bromopyruvate action by an electromagnetic field in vitro.

Research RF Safe Research Library Jan 1, 2022

This in vitro study tested continuous or pulsed ELF electromagnetic field exposure in human cancer cells treated with the anticancer agent 3-bromopyruvate (3BP). The authors report that ELF-EMF enhanced 3BP anti-cancer activity, which they link to reduced TNFα secretion, p21/p27-associated G2/M arrest, and cancer…

Translocation of silica nanospheres through giant unilamellar vesicles (GUVs) induced by a high frequency electromagnetic field.

Research RF Safe Research Library Jan 1, 2021

This in vitro study used giant unilamellar vesicles (GUVs) to examine effects of an 18 GHz electromagnetic field on membrane behavior. The authors report increased membrane permeability after exposure, inferred from internalisation of 23.5 nm hydrophilic silica nanospheres. Complementary molecular dynamics…

The Time-Dependence of Three Different Modes of ELF-EMF Stimulation on LTP at Schaffer Collateral-CA1 Synapses.

Research RF Safe Research Library Jan 1, 2021

This animal ex vivo study tested whether short-term 15 Hz/2 mT ELF-EMF exposure alters LTP at rat hippocampal SC-CA1 synapses. Across sinusoidal, pulsed, and rhythm-pulsed modes, the authors report reduced LTP versus control, with greater inhibition at longer exposure durations (10–60 s). Sinusoidal stimulation…

The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed mouse spermatogonial stem cells to 50 Hz EMF (2.5 mT; 1 h/day for 5 days) and assessed oxidative stress and cell health outcomes. The authors report increased ROS and apoptosis with reduced antioxidant (catalase) expression, viability, and colonization after EMF exposure. Sertoli…

Modulation of Crystallinity through Radiofrequency Electromagnetic Fields in PLLA/Magnetic Nanoparticles Composites: A Proof of Concept.

Research RF Safe Research Library Jan 1, 2021

This materials proof-of-concept study explored using radiofrequency EMF to heat magnetic nanoparticles dispersed in PLLA to modulate polymer properties. Heating was monitored by infrared camera, and polymer changes were assessed by differential scanning calorimetry and nanoindentation. The authors report that EMF…

Exposure to long-term evolution radiofrequency electromagnetic fields decreases neuroblastoma cell proliferation via Akt/mTOR-mediated cellular senescence.

Research RF Safe Research Library Jan 1, 2021

This in vitro study examined LTE RF-EMF exposure effects on SH-SY5Y neuroblastoma cells. The authors report reduced cell proliferation and a G0/G1 cell-cycle delay after 1760 MHz exposure at 4 W/kg for 4 hr/day over 4 days. They report no marked DNA damage signal (γH2AX) and no apoptosis activation, but increased…

Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed developing pre-B lymphocytes undergoing V(D)J recombination to low-intensity RF EMFs for 48 hours across 720–1224 MHz. The authors report a frequency- and dose-dependent decrease in recombination, with stronger reductions around 950–1000 MHz at relatively low power densities. γH2AX levels…

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