Electromagnetic field exposure-induced depression features could be alleviated by heat acclimation based on remodeling the gut microbiota

Abstract

Electromagnetic field exposure-induced depression features could be alleviated by heat acclimation based on remodeling the gut microbiota Luo X, Huang X, Luo Z, Wang Z, He G, Tan Y, Zhang B, Zhou H, Li P, Shen T, Yu X, Yang X. Electromagnetic field exposure-induced depression features could be alleviated by heat acclimation based on remodeling the gut microbiota. Ecotoxicol Environ Saf. 2021 Nov 15;228:112980. doi: 10.1016/j.ecoenv.2021.112980. Highlights • EMF exposure induced depression features. • EMF cause gut microbiota and metabolites disturbance similar to depression model. • HA alleviated depression caused by EMF. • HA alleviated depression by gut microbiota, might be mechanism of cross-tolerance. Abstract Background: Electromagnetic pollution cannot be ignored. Long-term low-dose electromagnetic field (EMF) exposure can cause central nervous system dysfunction without effective prevention. Materials/methods: Male C57BL/6J mice (6-8 weeks, 17-20 g) were used in this study. Depression-like and anxiety-like behaviors detected by behavioral experiments were compared among different treatments. 16S rRNA gene sequencing and non-targeted liquid chromatography-mass spectrometry (LC-MS) metabolomics were used to explore the relationship between EMF exposure and heat acclimation (HA) effects on gut microbes and serum metabolites. Results: Both EMF and HA regulated the proportions of p_Firmicutes and p_Bacteroidota. EMF exposure caused the proportions of 6 kinds of bacteria, such as g_Butyricicoccus and g_Anaerotruncus, to change significantly (p < 0.05). HA restored the balance of gut microbes that was affected by EMF exposure and the proportion of probiotics (g_Lactobacillus) increased significantly (p < 0.01). Serum metabolite analysis suggested that HA alleviated the disturbance of serum metabolites (such as cholesterol and D-mannose) induced by EMF exposure. Both the metabolic KEGG pathways and PICRUSt functional analysis demonstrated that tryptophan metabolism, pyrimidine metabolism and amino acid biosynthesis were involved. Conclusions: EMF exposure not only led to depression-like neurobehavioral disorders, but also to gut microbiota imbalance. HA alleviated the depression features caused by EMF exposure. Based on the analysis of gut microbiota associated with serum metabolites, we speculated that gut microbiota might play a vital role in the cross-tolerance provided by HA. pubmed.ncbi.nlm.nih.gov

AI evidence extraction

Main findings

In male C57BL/6J mice, long-term low-dose EMF exposure was associated with depression-like neurobehavioral features and gut microbiota imbalance, including significant changes in several bacterial genera. Heat acclimation (HA) restored gut microbial balance (including increased Lactobacillus) and alleviated EMF-associated disturbances in serum metabolites; pathway analyses implicated tryptophan metabolism, pyrimidine metabolism, and amino acid biosynthesis.

Outcomes measured

• Depression-like behaviors
• Anxiety-like behaviors
• Gut microbiota composition (16S rRNA sequencing)
• Serum metabolomics (non-targeted LC-MS)
• KEGG pathway analysis
• PICRUSt functional analysis

Limitations

• No EMF exposure parameters reported in abstract (e.g., frequency, intensity/SAR, exposure duration specifics)
• Sample size not reported in abstract
• Behavioral test details and effect sizes not reported in abstract
• Mechanistic role of gut microbiota described as speculative in conclusions

{
    "study_type": "animal",
    "exposure": {
        "band": null,
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "Long-term low-dose EMF exposure (details not specified)"
    },
    "population": "Male C57BL/6J mice (6–8 weeks, 17–20 g)",
    "sample_size": null,
    "outcomes": [
        "Depression-like behaviors",
        "Anxiety-like behaviors",
        "Gut microbiota composition (16S rRNA sequencing)",
        "Serum metabolomics (non-targeted LC-MS)",
        "KEGG pathway analysis",
        "PICRUSt functional analysis"
    ],
    "main_findings": "In male C57BL/6J mice, long-term low-dose EMF exposure was associated with depression-like neurobehavioral features and gut microbiota imbalance, including significant changes in several bacterial genera. Heat acclimation (HA) restored gut microbial balance (including increased Lactobacillus) and alleviated EMF-associated disturbances in serum metabolites; pathway analyses implicated tryptophan metabolism, pyrimidine metabolism, and amino acid biosynthesis.",
    "effect_direction": "mixed",
    "limitations": [
        "No EMF exposure parameters reported in abstract (e.g., frequency, intensity/SAR, exposure duration specifics)",
        "Sample size not reported in abstract",
        "Behavioral test details and effect sizes not reported in abstract",
        "Mechanistic role of gut microbiota described as speculative in conclusions"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electromagnetic field",
        "EMF exposure",
        "depression-like behavior",
        "anxiety-like behavior",
        "heat acclimation",
        "gut microbiota",
        "16S rRNA sequencing",
        "metabolomics",
        "LC-MS",
        "tryptophan metabolism",
        "PICRUSt",
        "KEGG"
    ],
    "suggested_hubs": []
}

Comments

Log in to comment.

No comments yet.