Hsp72-Based Effect and Mechanism of Microwave Radiation-Induced Cardiac Injury in Rats

PAPER manual 2022 Animal study Effect: harm Evidence: Low

Read original paper → DOI: 10.1155/2022/7145415 Evidence Lab

Abstract

Hsp72-Based Effect and Mechanism of Microwave Radiation-Induced Cardiac Injury in Rats Li D, Xu X, Gao Y, Wang J, Yin Y, Yao B, Zhao L, Wang H, Wang H, Dong J, Zhang J, Peng R. Hsp72-Based Effect and Mechanism of Microwave Radiation-Induced Cardiac Injury in Rats. Oxid Med Cell Longev. 2022 Aug 18;2022:7145415. doi: 10.1155/2022/7145415. Abstract The purpose of this study was to determine the role of heat shock protein 72 (Hsp72) changes in cardiac injury caused by microwave radiation, aimed at providing novel insights into the mechanism of this damage. A digital thermometer was used to measure the rectal temperature of the rats' pre- and post-radiation. On the 1st, 7th, 14th, and 28th days post-radiation, the changes in electrocardiogram (ECG) were analyzed by a multi-channel physiological recorder. The myocardial enzyme activities and ion concentrations were detected by an automatic biochemical analyzer. Additionally, the levels of myocardial injury markers were established by the enzyme- linked immunosorbent assay (ELISA), and those of hormones were measured by radioimmunoassay. The structure and ultrastructure of the myocardial tissue were observed using an optical microscope and transmission electron microscopy (TEM). The expression of Hsp72 was measured by Western blot and immunofluorescence analyses. Post-exposure, the rectal temperature in the R-group increased significantly, ECG was disordered, and the concentrations of ions were decreased. Furthermore, the activities of myocardial enzymes were changed, and the contents of myocardial injury markers and hormones were increased. We observed damage to the structure and ultrastructure and significantly increased expression of Hsp72. As a whole, the results indicated that S-wave microwave radiation at 30 mW/cm2 for 35 min resulted in damage to the cardiac functionality organigram, caused by a combination of the thermal and nonthermal effects. Open access paper: ncbi.nlm.nih.gov

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Rats

Sample size

—

Exposure

microwave · 35 min

Evidence strength

Low

Confidence: 78% · Peer-reviewed: unknown

Main findings

Post-exposure, rectal temperature increased significantly, ECG was disordered, ion concentrations decreased, myocardial enzyme activities changed, and myocardial injury markers and hormones increased. Structural and ultrastructural myocardial damage was observed along with significantly increased Hsp72 expression. The authors report that S-wave microwave radiation at 30 mW/cm2 for 35 min resulted in cardiac functional/structural injury, attributed to combined thermal and nonthermal effects.

Outcomes measured

Rectal temperature
Electrocardiogram (ECG) changes
Myocardial enzyme activities
Ion concentrations
Myocardial injury markers (ELISA)
Hormone levels (radioimmunoassay)
Myocardial tissue structure and ultrastructure (optical microscopy, TEM)
Hsp72 expression (Western blot, immunofluorescence)

Limitations

Sample size not stated in the provided abstract/metadata
Microwave frequency not stated in the provided abstract/metadata
No SAR reported in the provided abstract/metadata
Exposure source (device/context) not specified in the provided abstract/metadata

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "35 min"
    },
    "population": "Rats",
    "sample_size": null,
    "outcomes": [
        "Rectal temperature",
        "Electrocardiogram (ECG) changes",
        "Myocardial enzyme activities",
        "Ion concentrations",
        "Myocardial injury markers (ELISA)",
        "Hormone levels (radioimmunoassay)",
        "Myocardial tissue structure and ultrastructure (optical microscopy, TEM)",
        "Hsp72 expression (Western blot, immunofluorescence)"
    ],
    "main_findings": "Post-exposure, rectal temperature increased significantly, ECG was disordered, ion concentrations decreased, myocardial enzyme activities changed, and myocardial injury markers and hormones increased. Structural and ultrastructural myocardial damage was observed along with significantly increased Hsp72 expression. The authors report that S-wave microwave radiation at 30 mW/cm2 for 35 min resulted in cardiac functional/structural injury, attributed to combined thermal and nonthermal effects.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not stated in the provided abstract/metadata",
        "Microwave frequency not stated in the provided abstract/metadata",
        "No SAR reported in the provided abstract/metadata",
        "Exposure source (device/context) not specified in the provided abstract/metadata"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "unknown",
    "keywords": [
        "microwave radiation",
        "cardiac injury",
        "rats",
        "Hsp72",
        "heat shock protein 72",
        "ECG",
        "myocardial enzymes",
        "ion concentrations",
        "ELISA",
        "TEM",
        "thermal effects",
        "nonthermal effects"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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