Micronucleus assay and lymphocyte mitotic activity in risk assessment of occupational exposure to microwave radiation.

Abstract

The effects of radiofrequency electromagnetic radiation (RFR) on the cell kinetics and genome damages in peripheral blood lymphocytes were determined in lymphocytes of 12 subjects occupationally exposed to microwave radiation. Results showed an increase in frequency of micronuclei (MN) as well as disturbances in the distribution of cells over the first, second and third mitotic division in exposed subjects compared to controls. According to previous reports micronucleus assay can serve as a suitable indicator for the assessment of exposure to genotoxic agents (such as RFR) and the analysis of mitotic activity as an additional parameter for the efficient biomonitoring.

AI evidence extraction

Main findings

Compared to controls, occupationally exposed subjects showed an increased frequency of micronuclei and disturbances in the distribution of cells across the first, second, and third mitotic divisions.

Outcomes measured

• micronuclei frequency
• lymphocyte mitotic activity / cell kinetics (distribution across 1st, 2nd, 3rd mitotic divisions)
• genome damage (as assessed by micronucleus assay)

Limitations

• Exposure characteristics (frequency, intensity/SAR, duration) not reported in abstract
• Small exposed sample size (12 subjects)
• Study design details (selection of controls, confounding control) not described in abstract

Suggested hubs

• occupational-exposure (0.9)
  Study evaluates biomarkers in workers occupationally exposed to microwave/RF radiation.

{
    "study_type": "cohort",
    "exposure": {
        "band": "RF",
        "source": "occupational",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Peripheral blood lymphocytes from 12 subjects occupationally exposed to microwave radiation (with controls)",
    "sample_size": 12,
    "outcomes": [
        "micronuclei frequency",
        "lymphocyte mitotic activity / cell kinetics (distribution across 1st, 2nd, 3rd mitotic divisions)",
        "genome damage (as assessed by micronucleus assay)"
    ],
    "main_findings": "Compared to controls, occupationally exposed subjects showed an increased frequency of micronuclei and disturbances in the distribution of cells across the first, second, and third mitotic divisions.",
    "effect_direction": "harm",
    "limitations": [
        "Exposure characteristics (frequency, intensity/SAR, duration) not reported in abstract",
        "Small exposed sample size (12 subjects)",
        "Study design details (selection of controls, confounding control) not described in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7199999999999999733546474089962430298328399658203125,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "radiofrequency electromagnetic radiation",
        "microwave radiation",
        "occupational exposure",
        "micronucleus assay",
        "lymphocytes",
        "genotoxicity",
        "mitotic activity",
        "biomonitoring"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Study evaluates biomarkers in workers occupationally exposed to microwave/RF radiation."
        }
    ]
}

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