Electromagnetic field (50 Hz) enhance metabolic potential and induce adaptive/reprogramming response mediated by the increase of N6-methyladenosine RNA methylation in adipose-derived mesenchymal stem cells in vitro.

PAPER pubmed Toxicology in vitro : an international journal published in association with BIBRA 2024 In vitro study Effect: mixed Evidence: Low

Read original paper → DOI: 10.1016/j.tiv.2023.105743 PMID: 38040129 Evidence Lab

Abstract

BACKGROUND: Electromagnetic fields (EMF) have an impact on numerous cellular processes. It can positively and negatively affect adipose-derived stem cells (ASCs) thus their fate through the influence of specific factors and protein secretion. EMF can be a great factor for preconditioning ASCs for regenerative medicine purposes, however, understanding the cell's biological response to its effects in vitro is essential. METHODS: ASCs were exposed to the EMF (50 Hz; 1.5 mT) for 24 and 48 h, and then cell biological response was analyzed. RESULTS: 24 h exposure of ASCs to EMF, significantly increased N6-methyladenosine (mA) RNA methylation, indicating epitranscriptomic changes as an important factor in ASCs preconditioning. Furthermore, the expression of stem cell markers such as Nanog, Oct-4, Sox-2, CD44, and CD105 increased after 24 h of EMF exposure. Besides, western blot analysis showed upregulation of p21 and DNMT2/TRDMT1 protein levels compared to control cells with no differences in the p53 profile. Moreover, after 24 h of exposure to EMF, cell membrane flexibility, the metabolic potential of cells as well as the distribution, morphology, and metabolism of mitochondria were altered. CONCLUSION: ASCs undergo a process of mobilization and adaptation under the EMF influence through the increased mA RNA modifications. These conditions may "force" ASCs to redefine their stem cell fate mediated by RNA-modifying enzymes and alter their reprogramming decision of as differentiation begins.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

mixed

Population

Adipose-derived mesenchymal stem cells (ASCs) in vitro

Sample size

—

Exposure

ELF · 0.05 MHz · 24 and 48 h

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

ASCs exposed to 50 Hz, 1.5 mT EMF showed a significant increase in N6-methyladenosine RNA methylation after 24 h. After 24 h exposure, stem cell marker expression increased, p21 and DNMT2/TRDMT1 protein levels were upregulated with no differences in p53 profile, and cell membrane flexibility, metabolic potential, and mitochondrial characteristics were altered.

Outcomes measured

N6-methyladenosine (mA) RNA methylation
Stem cell marker expression (Nanog, Oct-4, Sox-2, CD44, CD105)
Protein levels (p21, DNMT2/TRDMT1, p53)
Cell membrane flexibility
Metabolic potential
Mitochondrial distribution, morphology, and metabolism

Limitations

In vitro study (cell culture), limiting direct inference to human health outcomes
Sample size not reported in the abstract
Results are described primarily for 24 h exposure; findings for 48 h are not detailed in the abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": "24 and 48 h"
    },
    "population": "Adipose-derived mesenchymal stem cells (ASCs) in vitro",
    "sample_size": null,
    "outcomes": [
        "N6-methyladenosine (mA) RNA methylation",
        "Stem cell marker expression (Nanog, Oct-4, Sox-2, CD44, CD105)",
        "Protein levels (p21, DNMT2/TRDMT1, p53)",
        "Cell membrane flexibility",
        "Metabolic potential",
        "Mitochondrial distribution, morphology, and metabolism"
    ],
    "main_findings": "ASCs exposed to 50 Hz, 1.5 mT EMF showed a significant increase in N6-methyladenosine RNA methylation after 24 h. After 24 h exposure, stem cell marker expression increased, p21 and DNMT2/TRDMT1 protein levels were upregulated with no differences in p53 profile, and cell membrane flexibility, metabolic potential, and mitochondrial characteristics were altered.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro study (cell culture), limiting direct inference to human health outcomes",
        "Sample size not reported in the abstract",
        "Results are described primarily for 24 h exposure; findings for 48 h are not detailed in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electromagnetic field",
        "ELF-EMF",
        "50 Hz",
        "1.5 mT",
        "adipose-derived stem cells",
        "mesenchymal stem cells",
        "N6-methyladenosine",
        "m6A",
        "RNA methylation",
        "epitranscriptomic changes",
        "preconditioning",
        "mitochondria",
        "metabolic potential",
        "p21",
        "DNMT2/TRDMT1"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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