Low-frequency electromagnetic field influences human oral mucosa keratinocyte viability in response to lipopolysaccharide or minocycline treatment in cell culture conditions.

PAPER pubmed Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 In vitro study Effect: mixed Evidence: Low

Read original paper → DOI: 10.1016/j.biopha.2021.111340 PMID: 33556878 Evidence Lab

Abstract

The aim of the current study was to investigate the influence of low-frequency electromagnetic field (LF-EMF) exposure on viability parameters of oral mucosa keratinocytes cultured in in vitro conditions. The effect of LF-EMF stimulation on cell viability was also specified in the simultaneous presence of lipopolysaccharide (LPS) infectious agent or minocycline (Mino) anti-inflammatory agent. Viability parameters such as early-, late apoptosis and necrosis of keratinocytes were analysed by the flow cytometry method (FCM). The exposure of human oral keratinocyte cell cultures to LF-EMF acting alone or combined with LPS/minocycline agents caused changes in the percentage of cells that undergo programmed or incidental cell death. The overall obtained results are compiled in a graphical form presented in Fig. 1.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

mixed

Population

Human oral mucosa keratinocytes (cell culture)

Sample size

—

Exposure

ELF

Evidence strength

Low

Confidence: 66% · Peer-reviewed: yes

Main findings

Exposure of human oral keratinocyte cultures to low-frequency electromagnetic field (LF-EMF), alone or combined with lipopolysaccharide (LPS) or minocycline, caused changes in the percentages of cells undergoing programmed (apoptosis) or incidental (necrosis) cell death.

Outcomes measured

Keratinocyte viability parameters
Early apoptosis
Late apoptosis
Necrosis

Limitations

In vitro cell culture study (no in vivo/clinical outcomes reported in abstract)
LF-EMF exposure characteristics (e.g., frequency, intensity, duration) not provided in abstract
Numerical results and direction of changes not reported in abstract (referenced as compiled in Fig. 1)
Sample size not reported in abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Human oral mucosa keratinocytes (cell culture)",
    "sample_size": null,
    "outcomes": [
        "Keratinocyte viability parameters",
        "Early apoptosis",
        "Late apoptosis",
        "Necrosis"
    ],
    "main_findings": "Exposure of human oral keratinocyte cultures to low-frequency electromagnetic field (LF-EMF), alone or combined with lipopolysaccharide (LPS) or minocycline, caused changes in the percentages of cells undergoing programmed (apoptosis) or incidental (necrosis) cell death.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro cell culture study (no in vivo/clinical outcomes reported in abstract)",
        "LF-EMF exposure characteristics (e.g., frequency, intensity, duration) not provided in abstract",
        "Numerical results and direction of changes not reported in abstract (referenced as compiled in Fig. 1)",
        "Sample size not reported in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.66000000000000003108624468950438313186168670654296875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "low-frequency electromagnetic field",
        "LF-EMF",
        "oral mucosa",
        "keratinocytes",
        "cell viability",
        "apoptosis",
        "necrosis",
        "lipopolysaccharide",
        "minocycline",
        "flow cytometry"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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