The Dynamic(s) of Adipose Stem Cell System, Their Survival, and Cessation under the Influence of Electromagnetic Fields.

PAPER pubmed Journal of medical physics 2021 In vitro study Effect: mixed Evidence: Low

Read original paper → DOI: 10.4103/jmp.jmp_10_21 PMID: 34703098 Evidence Lab

Abstract

CONTEXT: The electromagnetic field (EMF) is one of the external biophysical factors that can influence stem cells' structure and functionality. Depending on its frequency and magnetic flux density, EMF can have both a positive and negative effect on stem cell biology. AIMS: The aim of the study is to define EMF conditions that support beneficial physiological processes and those that lead to pathophysiological phenomena. Understanding the changes and processes occurring in stem cells after exposure to EMFs of different parameters can be an important factor to be applied in stem cell-based therapies and regenerative medicine. MATERIALS AND METHODS: In this study, using fluorescent microscopy and flow cytometry methods, the influence of EMF on adipose-derived stem cells proliferation, cell cycle, viability, and death were examined. EMF parameters were set in accordance with the ion cyclotron resonance (ICR) theory that influences Ca and Mg ions influx. Results were statistically developed using the ANOVA and effect size (Cohen's d) analyses. RESULTS: In this study, the continuous exposure of adipose-derived stem cells to EMF (ICR parameters: 76.6 Hz; 20 μT) causes a statistically significant increase in cell death through the enhancement of apoptotic, necrotic, and autophagic cell numbers. Apart from increased cell deaths after EMF exposure, increased proliferation after 24 h of EMF exposure has been also observed. CONCLUSIONS: Results presented in this study show that EMF influences stem cell dynamics resulting in a significantly increased cell death, thus altering the stem cell fate. It is important to further establish EMF conditions that support ASCs functioning and beneficial physiological processes for future regenerative medical purposes.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

mixed

Population

Adipose-derived stem cells (ASCs)

Sample size

—

Exposure

ELF · continuous; 24 h mentioned for proliferation observation

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

Continuous exposure of adipose-derived stem cells to EMF set to ion cyclotron resonance parameters (76.6 Hz; 20 μT) was associated with a statistically significant increase in cell death, with increased apoptotic, necrotic, and autophagic cell numbers. Increased proliferation after 24 h of EMF exposure was also observed.

Outcomes measured

cell proliferation
cell cycle
cell viability
cell death
apoptosis
necrosis
autophagy

Limitations

Sample size not reported in abstract
Exposure duration and full exposure protocol details not fully specified in abstract
In vitro study; generalizability to in vivo/clinical settings not addressed in abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "continuous; 24 h mentioned for proliferation observation"
    },
    "population": "Adipose-derived stem cells (ASCs)",
    "sample_size": null,
    "outcomes": [
        "cell proliferation",
        "cell cycle",
        "cell viability",
        "cell death",
        "apoptosis",
        "necrosis",
        "autophagy"
    ],
    "main_findings": "Continuous exposure of adipose-derived stem cells to EMF set to ion cyclotron resonance parameters (76.6 Hz; 20 μT) was associated with a statistically significant increase in cell death, with increased apoptotic, necrotic, and autophagic cell numbers. Increased proliferation after 24 h of EMF exposure was also observed.",
    "effect_direction": "mixed",
    "limitations": [
        "Sample size not reported in abstract",
        "Exposure duration and full exposure protocol details not fully specified in abstract",
        "In vitro study; generalizability to in vivo/clinical settings not addressed in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electromagnetic field",
        "ELF-EMF",
        "ion cyclotron resonance",
        "76.6 Hz",
        "20 μT",
        "adipose-derived stem cells",
        "proliferation",
        "apoptosis",
        "necrosis",
        "autophagy",
        "cell viability",
        "flow cytometry",
        "fluorescent microscopy"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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