Enhancement in the ATP level and antioxidant capacity of under continuous exposure to extremely low-frequency electromagnetic field for multiple generations.

Abstract

PURPOSE: Safety concerns about the effects of long-term extremely low-frequency electromagnetic field (ELF-EMF) exposure on human health have been raised. To explore the effects of continuous exposure to ELF-EMF on organisms for multiple generations, we selected as a model organism and conducted long-term continuous exposure studies for multiple generations under 20 °C, 50 Hz, and 3 mT ELF-EMF. MATERIALS AND METHODS: Each generation of worms was treated with ELF-EMF from the egg in the same environment. After long-term exposure to ELF-EMF, the body length of the worms was detected, and 15th generation adult worms were selected as the research object. The ATP level and ATPase were detected, and the expression levels of genes encoding ATP synthase () were detected by RT-PCR. In worm's antioxidant system, the level of reactive oxygen species (ROS) was detected by dichlorofluorescein staining, and the total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and catalase (CAT) activity were investigated. The expression of genes encoding superoxide dismutase () was detected in adult (60 h) worms of the fifteenth generation (F15). RESULTS: These results showed that the body length of F15 worms increased significantly, ATP content increased significantly, ATP synthase activity was significantly enhanced, and the expression levels of the , and genes encoding ATPase were significantly upregulated in F15 worms. In addition, SOD activity increased significantly, and the expression levels of the and genes encoding SOD were also significantly upregulated in F15 worms. CONCLUSIONS: These results indicated that continuous exposure to 50 Hz, 3 mT ELF-EMF for multiple generations can increase the body length of worms, induce the synthesis of ATP and enhance the antioxidant capacity of worms.

AI evidence extraction

Main findings

Under continuous 50 Hz, 3 mT ELF-EMF exposure across generations, 15th-generation worms had significantly increased body length, ATP content, ATP synthase activity, and upregulated expression of genes encoding ATPase/ATP synthase. SOD activity and expression of SOD-encoding genes were also significantly increased.

Outcomes measured

• Body length
• ATP content/level
• ATP synthase activity
• Expression of genes encoding ATPase/ATP synthase (RT-PCR)
• Reactive oxygen species (ROS) level
• Total antioxidant capacity (T-AOC)
• Superoxide dismutase (SOD) activity
• Catalase (CAT) activity
• Expression of genes encoding SOD

Limitations

• Model organism species not specified in the provided abstract text
• Sample size and statistical details not provided in the abstract
• Only 15th-generation adults were selected for detailed biochemical/gene expression analyses (per abstract)
• Exposure conditions include temperature (20°C) which may limit generalizability

Suggested hubs

• elF-emf (0.9)
  Study involves extremely low-frequency EMF exposure at 50 Hz.

{
    "study_type": "animal",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": "continuous exposure from egg across multiple generations; outcomes assessed in 15th generation (F15)"
    },
    "population": "Worms (model organism; species not specified in abstract)",
    "sample_size": null,
    "outcomes": [
        "Body length",
        "ATP content/level",
        "ATP synthase activity",
        "Expression of genes encoding ATPase/ATP synthase (RT-PCR)",
        "Reactive oxygen species (ROS) level",
        "Total antioxidant capacity (T-AOC)",
        "Superoxide dismutase (SOD) activity",
        "Catalase (CAT) activity",
        "Expression of genes encoding SOD"
    ],
    "main_findings": "Under continuous 50 Hz, 3 mT ELF-EMF exposure across generations, 15th-generation worms had significantly increased body length, ATP content, ATP synthase activity, and upregulated expression of genes encoding ATPase/ATP synthase. SOD activity and expression of SOD-encoding genes were also significantly increased.",
    "effect_direction": "benefit",
    "limitations": [
        "Model organism species not specified in the provided abstract text",
        "Sample size and statistical details not provided in the abstract",
        "Only 15th-generation adults were selected for detailed biochemical/gene expression analyses (per abstract)",
        "Exposure conditions include temperature (20°C) which may limit generalizability"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "50 Hz",
        "3 mT",
        "multigenerational exposure",
        "worms",
        "ATP",
        "ATP synthase",
        "ATPase genes",
        "antioxidant capacity",
        "SOD",
        "ROS",
        "T-AOC",
        "CAT"
    ],
    "suggested_hubs": [
        {
            "slug": "elF-emf",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Study involves extremely low-frequency EMF exposure at 50 Hz."
        }
    ]
}

Comments

Log in to comment.

No comments yet.