Morphological and cytophysiological changes in selected lines of normal and cancer human cells under the influence of a radio-frequency electromagnetic field.

Abstract

INTRODUCTION: Currently, mobile phones and Wi-Fi are the most commonly used forms of telecommunication. The popularity of mobile telecommunications has made it necessary to investigate the problem more comprehensively and cautiously assess the possible risks, because never before in history has such a substantial proportion of the population been exposed to microwaves at comparably high levels. Some studies indicate that the high frequency electromagnetic radiation emitted by mobile phone and Wi-Fi connections can have a negative effect on human health, and can cause cancer. OBJECTIVE: The aim of the study was to investigate the influence of the radiofrquency electromagnetic field (RF-EMF) on the metaboloc activity and morphology of normal human cells (fibroblasts) and cancer cells (prostate cancer cells). MATERIAL AND METHODS: The cell cultures (human fibroblasts and prostate cancer cells) were exposed to RF-EMF at the frequency of 2.5 GHz for 24, 48 and 72h. To quantify changes in cell viability, the Cell Counting Kit - 8 was used. RESULTS: It was found that the RF electromagnetic field exposure caused a significant decrease in the viability of fibroblasts, and a significant increase in cancer cells. Morphological analysis did not show significant changes in both cell lines after exposure to RF-EMF. CONCLUSIONS: On the basis of the obtained results, the hypothesis can be formulated that a high frequency electromagnetic field can have harmful effects on human cells.

AI evidence extraction

Main findings

Exposure of human fibroblasts and prostate cancer cells to 2.5 GHz RF-EMF for 24, 48, and 72 hours was associated with a significant decrease in fibroblast viability and a significant increase in prostate cancer cell viability. Morphological analysis did not show significant changes in either cell line after RF-EMF exposure.

Outcomes measured

• Cell viability (metabolic activity)
• Cell morphology

Limitations

• In vitro cell culture study; findings may not translate to human health outcomes
• No SAR or exposure intensity metrics reported in the abstract
• Sample size and detailed exposure setup not reported in the abstract
• Only two cell lines assessed (fibroblasts and prostate cancer cells)
• Morphological assessment described without methodological detail in the abstract

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "RF",
        "source": "mobile phone / Wi-Fi (context)",
        "frequency_mhz": 2500,
        "sar_wkg": null,
        "duration": "24, 48 and 72 h"
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Cell viability (metabolic activity)",
        "Cell morphology"
    ],
    "main_findings": "Exposure of human fibroblasts and prostate cancer cells to 2.5 GHz RF-EMF for 24, 48, and 72 hours was associated with a significant decrease in fibroblast viability and a significant increase in prostate cancer cell viability. Morphological analysis did not show significant changes in either cell line after RF-EMF exposure.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro cell culture study; findings may not translate to human health outcomes",
        "No SAR or exposure intensity metrics reported in the abstract",
        "Sample size and detailed exposure setup not reported in the abstract",
        "Only two cell lines assessed (fibroblasts and prostate cancer cells)",
        "Morphological assessment described without methodological detail in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "RF-EMF",
        "2.5 GHz",
        "microwaves",
        "Wi-Fi",
        "mobile phones",
        "fibroblasts",
        "prostate cancer cells",
        "cell viability",
        "morphology",
        "in vitro"
    ],
    "suggested_hubs": []
}

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