Effects of 5-HT and 5-HT Receptor Agonists on Electromagnetic Field-Induced Analgesia in Rats.

PAPER pubmed Bioelectromagnetics 2019 Animal study Effect: benefit Evidence: Low

Read original paper → DOI: 10.1002/bem.22196 PMID: 31152464 Evidence Lab

Abstract

Much evidence demonstrates the antinociceptive effect of magnetic fields (MFs). However, the analgesic action mechanism of the electromagnetic field (EMF) is not exactly understood. The aim of the present study was to investigate the effects of 5-HT and 5-HT receptor agonists (serotonin HCl and 2,5-dimethoxy-4-iodoamphetamine [DOI] hydrochloride) on EMF-induced analgesia. In total, 66 adult male Wistar albino rats with an average body mass of 225 ± 13 g were used in this study. The animals were subjected to repeated exposures of alternating 50 Hz and 5 mT EMF for 2 h a day for 15 days. Prior to analgesia tests, serotonin HCl (5-HT agonist) 4 mg/kg, WAY 100635 (5-HT antagonist) 0.04 mg/kg, DOI hydrochloride (5-HT receptor agonist) 4 mg/kg, and SB 204741 (5-HT antagonist) 0.5 mg/kg doses were injected into rats. For statistical analysis of the data, analysis of variance was used and multiple comparisons were determined by Tukey's test. Administration of serotonin HCl MF (5 mT)-exposed rats produced a significant increase in percent maximal possible effect (% MPE) as compared with EMF group (P < 0.05). On the contrary, injection of WAY 100635 to MF-exposed rats produced a significant decrease in analgesic activity (P < 0.05). Similarly, the administration of DOI hydrochloride significantly increased % MPE values as compared with the EMF group while SB 204741 reduced it (P < 0.05). In conclusion, our results suggested that serotonin 5-HT and 5-HT receptors play an important role in EMF-induced analgesia; however, further research studies are necessary to understand the mechanism. Bioelectromagnetics. 2019;40:319-330. © 2019 Bioelectromagnetics Society.

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

benefit

Population

Adult male Wistar albino rats

Sample size

Exposure

ELF · 2 h/day for 15 days; alternating 50 Hz, 5 mT

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

Rats exposed to alternating 50 Hz, 5 mT EMF showed analgesic activity that was increased by serotonin HCl and DOI hydrochloride compared with the EMF group, while WAY 100635 and SB 204741 reduced analgesic activity in MF-exposed rats (all P<0.05). The authors conclude that 5-HT and 5-HT receptors play an important role in EMF-induced analgesia.

Outcomes measured

Analgesia/antinociception (% maximal possible effect, %MPE)
Effect of serotonergic drugs (5-HT agonist/antagonists; 5-HT receptor agonist/antagonist) on EMF-induced analgesia

Limitations

Analgesia mechanism described as not exactly understood
Authors state further research is necessary to understand the mechanism
Exposure described as alternating 50 Hz and 5 mT; other exposure characterization details not provided in abstract

Suggested hubs

animal-studies (0.9)
In vivo rat experiment assessing EMF-induced analgesia and serotonergic modulation.

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "2 h/day for 15 days; alternating 50 Hz, 5 mT"
    },
    "population": "Adult male Wistar albino rats",
    "sample_size": 66,
    "outcomes": [
        "Analgesia/antinociception (% maximal possible effect, %MPE)",
        "Effect of serotonergic drugs (5-HT agonist/antagonists; 5-HT receptor agonist/antagonist) on EMF-induced analgesia"
    ],
    "main_findings": "Rats exposed to alternating 50 Hz, 5 mT EMF showed analgesic activity that was increased by serotonin HCl and DOI hydrochloride compared with the EMF group, while WAY 100635 and SB 204741 reduced analgesic activity in MF-exposed rats (all P<0.05). The authors conclude that 5-HT and 5-HT receptors play an important role in EMF-induced analgesia.",
    "effect_direction": "benefit",
    "limitations": [
        "Analgesia mechanism described as not exactly understood",
        "Authors state further research is necessary to understand the mechanism",
        "Exposure described as alternating 50 Hz and 5 mT; other exposure characterization details not provided in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electromagnetic field",
        "magnetic field",
        "ELF",
        "50 Hz",
        "5 mT",
        "analgesia",
        "antinociception",
        "serotonin",
        "5-HT",
        "DOI",
        "WAY 100635",
        "SB 204741",
        "rat"
    ],
    "suggested_hubs": [
        {
            "slug": "animal-studies",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "In vivo rat experiment assessing EMF-induced analgesia and serotonergic modulation."
        }
    ]
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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