Electromagnetic Field Could Protect SH-SY5Y Cells Against Cisplatin Cytotoxicity, But Not MCF-7 Cells.

Abstract

Cisplatin [cis-dichlorodiammine platinum (II), CDDP], morphine (Mor), and electromagnetic field (EMF) induced oxidative stress. In this study, we tried to increase the cytotoxicity of CDDP in combination with Mor and/or EMF in MCF-7 and SH-SY5Y cells. Furthermore, we evaluate the expression levels of 11 antioxidant genes in both cell lines. We designed four treatments: CDDP alone, "CDDP+Mor," "CDDP+EMF," and "CDDP+Mor+EMF." Serial dilutions of CDDP, Mor (5.0 μM), and EMF (50 Hz, 0.50 mT, "15 min field-on/15 min field-off") were used for estimation of relative IC values. The mRNA expression levels of antioxidant genes were determined by real-time PCR. The IC value of CDDP in "CDDP+Mor+EMF" treatment was significantly higher than CDDP alone and "CDDP+Mor" treatments in both cell lines. Whereas the expression levels of antioxidant genes in the four treatments showed similar patterns in MCF-7 cells, in SH-SY5Y cells, most of the antioxidant genes showed an upregulation with "CDDP+EMF" and "CDDP+Mor+EMF" treatments. Moreover, significant differences in the number of upregulated genes were observed between different treatments in SH-SY5Y cells. The molecular mechanism of CDDP-reduced cytotoxicity in our designed combinations is probably different in MCF-7 and SH-SY5Y cells. CDDP in combination with EMF could protect SH-SY5Y cells from the cytotoxicity, whereas it has no significant change in MCF-7 cells.

AI evidence extraction

Main findings

In both MCF-7 and SH-SY5Y cells, the IC value of cisplatin in the CDDP+Mor+EMF condition was significantly higher than with CDDP alone and CDDP+Mor. In SH-SY5Y cells, most antioxidant genes were upregulated with CDDP+EMF and CDDP+Mor+EMF, while MCF-7 showed similar antioxidant gene expression patterns across treatments. The authors report that EMF in combination with CDDP could protect SH-SY5Y cells from cytotoxicity, with no significant change in MCF-7 cells.

Outcomes measured

• Cisplatin (CDDP) cytotoxicity (IC values)
• mRNA expression levels of 11 antioxidant genes (real-time PCR)

Limitations

• In vitro study (cell lines), not directly generalizable to humans
• Sample size/replicates not reported in abstract
• Exposure details limited to 50 Hz, 0.50 mT and duty cycle; total exposure duration not specified
• Only antioxidant gene mRNA expression assessed (no protein/activity measures described in abstract)

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "other",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "15 min field-on/15 min field-off"
    },
    "population": "MCF-7 and SH-SY5Y cell lines",
    "sample_size": null,
    "outcomes": [
        "Cisplatin (CDDP) cytotoxicity (IC values)",
        "mRNA expression levels of 11 antioxidant genes (real-time PCR)"
    ],
    "main_findings": "In both MCF-7 and SH-SY5Y cells, the IC value of cisplatin in the CDDP+Mor+EMF condition was significantly higher than with CDDP alone and CDDP+Mor. In SH-SY5Y cells, most antioxidant genes were upregulated with CDDP+EMF and CDDP+Mor+EMF, while MCF-7 showed similar antioxidant gene expression patterns across treatments. The authors report that EMF in combination with CDDP could protect SH-SY5Y cells from cytotoxicity, with no significant change in MCF-7 cells.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro study (cell lines), not directly generalizable to humans",
        "Sample size/replicates not reported in abstract",
        "Exposure details limited to 50 Hz, 0.50 mT and duty cycle; total exposure duration not specified",
        "Only antioxidant gene mRNA expression assessed (no protein/activity measures described in abstract)"
    ],
    "evidence_strength": "very_low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "50 Hz",
        "0.50 mT",
        "cisplatin",
        "morphine",
        "MCF-7",
        "SH-SY5Y",
        "oxidative stress",
        "antioxidant genes",
        "cytotoxicity",
        "IC value"
    ],
    "suggested_hubs": []
}

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