Effects of electromagnetic field, cisplatin and morphine on cytotoxicity and expression levels of DNA repair genes.

PAPER pubmed Molecular biology reports 2018 In vitro study Effect: mixed Evidence: Very low

Read original paper → DOI: 10.1007/s11033-018-4223-7 PMID: 29968116 Evidence Lab

Abstract

Morphine (Mor) is widely used as an analgesic drug in cancers and in combination with chemotherapy is known to have DNA damaging effects on non-targeted cell. This study surveyed the effect of Mor in combination with 50-Hz electromagnetic field (EMF) and co-treatment of cisplatin in combination with Mor and EMF on the expression of genes involved in DNA repair pathways. MCF-7 and SH-SY5Y cells were treated with 5.0 µM Mor and then exposed to 50-Hz 0.50 mT EMF in the intermittent pattern of 15 min field-on/15 min field-off. Gene expression, cisplatin and bleomycin cytotoxicity were measured using real-time PCR and MTT assay. Mor treated cells showed significant down-regulation of the examined genes, while in "Mor + EMF" treatments the genes were not significantly changed. IC of cisplatin was significantly elevated in both cell lines when co-treated with "Mor + EMF" compared with Mor treated cells. Non-homologous end joining (NHEJ) related genes were significantly decreased in co-treatment of cisplatin and "Mor + EMF" which led to bleomycin higher cytotoxicity in SH-SY5Y not in MCF-7. Our data is promising for providing a cell line-specific sensitization by combination of cisplatin and "Mor + EMF" treatment with local administration of double strand breaking agents.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

mixed

Population

MCF-7 and SH-SY5Y cell lines

Sample size

—

Exposure

ELF · 0.05 MHz · intermittent 15 min on/15 min off (overall duration not stated)

Evidence strength

Very low

Confidence: 74% · Peer-reviewed: yes

Main findings

Morphine (5.0 µM) treatment significantly down-regulated examined DNA repair genes, while morphine + 50-Hz 0.50 mT EMF did not significantly change these genes. The IC of cisplatin was significantly elevated in both cell lines with morphine + EMF co-treatment compared with morphine alone. In cisplatin plus morphine + EMF co-treatment, NHEJ-related genes were significantly decreased and bleomycin cytotoxicity was higher in SH-SY5Y but not in MCF-7.

Outcomes measured

Expression of DNA repair pathway genes (real-time PCR)
Cisplatin cytotoxicity / IC (MTT assay)
Bleomycin cytotoxicity (MTT assay)

Limitations

In vitro study (cell lines only)
Exposure duration not fully specified (only intermittent pattern described)
Specific DNA repair genes examined are not listed in the abstract
Sample size/replicates not reported in the abstract

Suggested hubs

elf-emf (0.9)
Uses 50-Hz (ELF) magnetic field exposure at 0.50 mT in an in vitro experiment.

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": "intermittent 15 min on/15 min off (overall duration not stated)"
    },
    "population": "MCF-7 and SH-SY5Y cell lines",
    "sample_size": null,
    "outcomes": [
        "Expression of DNA repair pathway genes (real-time PCR)",
        "Cisplatin cytotoxicity / IC (MTT assay)",
        "Bleomycin cytotoxicity (MTT assay)"
    ],
    "main_findings": "Morphine (5.0 µM) treatment significantly down-regulated examined DNA repair genes, while morphine + 50-Hz 0.50 mT EMF did not significantly change these genes. The IC of cisplatin was significantly elevated in both cell lines with morphine + EMF co-treatment compared with morphine alone. In cisplatin plus morphine + EMF co-treatment, NHEJ-related genes were significantly decreased and bleomycin cytotoxicity was higher in SH-SY5Y but not in MCF-7.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro study (cell lines only)",
        "Exposure duration not fully specified (only intermittent pattern described)",
        "Specific DNA repair genes examined are not listed in the abstract",
        "Sample size/replicates not reported in the abstract"
    ],
    "evidence_strength": "very_low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "50-Hz electromagnetic field",
        "ELF-EMF",
        "0.50 mT",
        "morphine",
        "cisplatin",
        "bleomycin",
        "DNA repair genes",
        "NHEJ",
        "MCF-7",
        "SH-SY5Y",
        "cytotoxicity",
        "real-time PCR",
        "MTT assay"
    ],
    "suggested_hubs": [
        {
            "slug": "elf-emf",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Uses 50-Hz (ELF) magnetic field exposure at 0.50 mT in an in vitro experiment."
        }
    ]
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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