Blueberry anthocyanins regulate SIRT1/FoxO1 pathway to inhibit oxidative stress and reduce

PAPER manual Journal of Functional Foods 2024 Animal study Effect: mixed Evidence: Low

Read original paper → DOI: 10.1016/j.jff.2024.106523 Evidence Lab

Abstract

Blueberry anthocyanins regulate SIRT1/FoxO1 pathway to inhibit oxidative stress and reduce testicular tissue damage induced by microwave radiation in rats Pang Y, Men J, Li Y, Zhang J, Zhao L, et al. Blueberry anthocyanins regulate SIRT1/FoxO1 pathway to inhibit oxidative stress and reduce testicular tissue damage induced by microwave radiation in rats. Journal of Functional Foods. Vol. 122, 2024. doi: 10.1016/j.jff.2024.106523. Abstract Researches have shown that microwave radiation could cause oxidative stress injury in male reproductive system, and blueberry anthocyanins had excellent oxidation resistance. Our study aimed to investigate the protective effect of blueberry anthocyanins (100, 200 and 400 mg/kg/d) on testicular tissue damage in Wistar rats exposed to 2.856 GHz microwave and the optimal dose. We found that blueberry anthocyanins could ameliorate the decrease of sperm motility and sex hormone levels and testicular tissue structure damage caused by microwave radiation, increase SIRT1 expression and decrease FoxO1 expression, increase GSH/GSSG, SOD and inhibit MDA. The LDH, SDH and ATP synthase were increased, and Caspase-3 expression was decreased, and the high-dose of blueberry anthocyanins (400 mg/kg/d) had the best protective effect. These results suggested that blueberry anthocyanins could inhibit oxidative stress injury induced by 2.856 GHz microwave radiation in rat testicular tissue by regulating SIRT1/FoxO1 pathway, enhance energy metabolism and reduce cell apoptosis. Open access paper: sciencedirect.com

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

mixed

Population

Wistar rats

Sample size

—

Exposure

microwave · 2856 MHz

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

In Wistar rats exposed to 2.856 GHz microwave radiation, blueberry anthocyanins (100, 200, 400 mg/kg/d) were reported to ameliorate decreases in sperm motility and sex hormone levels and reduce testicular tissue structural damage. Anthocyanins were associated with increased SIRT1 expression, decreased FoxO1 expression, improved oxidative stress markers (increased GSH/GSSG and SOD; inhibited MDA), increased LDH/SDH/ATP synthase, and decreased Caspase-3 expression; 400 mg/kg/d showed the best protective effect.

Outcomes measured

Testicular tissue damage/structure
Sperm motility
Sex hormone levels
Oxidative stress markers (GSH/GSSG, SOD, MDA)
SIRT1 expression
FoxO1 expression
Energy metabolism markers (LDH, SDH, ATP synthase)
Apoptosis marker (Caspase-3 expression)

Limitations

Sample size not reported in the provided abstract/metadata
Exposure duration and exposure metrics beyond frequency (e.g., SAR/power density) not reported
Details of randomization/blinding and control conditions not reported
Findings are from an animal model; human relevance not established in the provided text

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 2856,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Wistar rats",
    "sample_size": null,
    "outcomes": [
        "Testicular tissue damage/structure",
        "Sperm motility",
        "Sex hormone levels",
        "Oxidative stress markers (GSH/GSSG, SOD, MDA)",
        "SIRT1 expression",
        "FoxO1 expression",
        "Energy metabolism markers (LDH, SDH, ATP synthase)",
        "Apoptosis marker (Caspase-3 expression)"
    ],
    "main_findings": "In Wistar rats exposed to 2.856 GHz microwave radiation, blueberry anthocyanins (100, 200, 400 mg/kg/d) were reported to ameliorate decreases in sperm motility and sex hormone levels and reduce testicular tissue structural damage. Anthocyanins were associated with increased SIRT1 expression, decreased FoxO1 expression, improved oxidative stress markers (increased GSH/GSSG and SOD; inhibited MDA), increased LDH/SDH/ATP synthase, and decreased Caspase-3 expression; 400 mg/kg/d showed the best protective effect.",
    "effect_direction": "mixed",
    "limitations": [
        "Sample size not reported in the provided abstract/metadata",
        "Exposure duration and exposure metrics beyond frequency (e.g., SAR/power density) not reported",
        "Details of randomization/blinding and control conditions not reported",
        "Findings are from an animal model; human relevance not established in the provided text"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave radiation",
        "2.856 GHz",
        "testis",
        "male reproductive system",
        "oxidative stress",
        "blueberry anthocyanins",
        "SIRT1",
        "FoxO1",
        "sperm motility",
        "sex hormones",
        "GSH/GSSG",
        "SOD",
        "MDA",
        "LDH",
        "SDH",
        "ATP synthase",
        "Caspase-3",
        "apoptosis"
    ],
    "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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