Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways.

PAPER pubmed PloS one 2017 In vitro study Effect: benefit Evidence: Low

Read original paper → DOI: 10.1371/journal.pone.0173877 PMID: 28339498 Evidence Lab

Abstract

Zinc ion as an essential trace element and electromagnetic fields (EMFs) has been reported to be involved in the regulation of bone metabolism. The aim of this study was to elucidate the effects of zinc sulphate (ZnSO4) on the osteogenic differentiation of adipose tissue-derived mesenchymal stem cells (ADSCs) in the presence of EMF as a strategy in osteoporosis therapy. Alkaline phophatase (ALP) activity measurement, calcium assay and expression of several osteoblastic marker genes were examined to assess the effect of ZnSO4 on the osteogenic differentiation of ADSCs under EMF. The expression of cAMP and PKA was evaluated by ELISA. The expression of β-catenin, Wnt1, Wnt3a, low-density lipoprotein receptor-related protein 5 (LRP5) and reduced dickkopf1 (DKK1) genes were used to detect the Wnt/β-catenin pathway. It was found that ZnSO4, in the presence of EMF, resulted in an increase in the expression of osteogenic genes, ALP activity and calcium levels. EMF, in the presence of ZnSO4, increased the cAMP level and protein kinase A (PKA) activity. Treatment of ADSCs with (MAPK)/ERK kinase 1/2 inhibitor, or PKA inhibitor, significantly inhibited the promotion of osteogenic markers, indicating that the induction of osteogenesis was dependent on the ERK and PKA signaling pathways. Real-time PCR analysis showed that ZnSO4, in the presence of EMF, increased the mRNA expressions of β-catenin, Wnt1, Wnt3a, LRP5 and DKK1. In this study, it was shown that 0.432 μg/ml ZnSO4, in the presence of 50 Hz, 20 mT EMF, induced the osteogenic differentiation of ADSCs via PKA, ERK1/2 and Wnt/β-catenin signaling pathways.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

benefit

Population

Rat adipose tissue-derived mesenchymal stem cells (ADSCs)

Sample size

—

Exposure

ELF

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

ZnSO4 (0.432 μg/ml) in the presence of a 50 Hz, 20 mT EMF increased osteogenic gene expression, ALP activity, and calcium levels in rat ADSCs. Under these conditions, cAMP and PKA activity increased, and inhibitors of MEK/ERK1/2 or PKA inhibited the promotion of osteogenic markers; mRNA expression of β-catenin, Wnt1, Wnt3a, LRP5 and DKK1 also increased.

Outcomes measured

Osteogenic differentiation markers (osteogenic gene expression)
Alkaline phosphatase (ALP) activity
Calcium levels
cAMP level
Protein kinase A (PKA) activity
mRNA expression of β-catenin, Wnt1, Wnt3a, LRP5, DKK1 (Wnt/β-catenin pathway indicators)

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Rat adipose tissue-derived mesenchymal stem cells (ADSCs)",
    "sample_size": null,
    "outcomes": [
        "Osteogenic differentiation markers (osteogenic gene expression)",
        "Alkaline phosphatase (ALP) activity",
        "Calcium levels",
        "cAMP level",
        "Protein kinase A (PKA) activity",
        "mRNA expression of β-catenin, Wnt1, Wnt3a, LRP5, DKK1 (Wnt/β-catenin pathway indicators)"
    ],
    "main_findings": "ZnSO4 (0.432 μg/ml) in the presence of a 50 Hz, 20 mT EMF increased osteogenic gene expression, ALP activity, and calcium levels in rat ADSCs. Under these conditions, cAMP and PKA activity increased, and inhibitors of MEK/ERK1/2 or PKA inhibited the promotion of osteogenic markers; mRNA expression of β-catenin, Wnt1, Wnt3a, LRP5 and DKK1 also increased.",
    "effect_direction": "benefit",
    "limitations": [],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "electromagnetic field",
        "ELF-EMF",
        "50 Hz",
        "20 mT",
        "zinc sulphate",
        "ZnSO4",
        "osteogenic differentiation",
        "mesenchymal stem cells",
        "ADSCs",
        "PKA",
        "ERK1/2",
        "Wnt/β-catenin",
        "β-catenin",
        "Wnt1",
        "Wnt3a",
        "LRP5",
        "DKK1",
        "osteoporosis"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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