Rat adipose-derived mesenchymal stem cells aging reduction by zinc sulfate under extremely low frequency electromagnetic field exposure is associated with increased telomerase reverse transcriptase gene expression.

PAPER pubmed Veterinary research forum : an international quarterly journal 2017 In vitro study Effect: mixed Evidence: Low

Read original paper → PMID: 28785382 Evidence Lab

Abstract

Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in the presence of zinc sulfate (ZnSO) affects the telomerase reverse transcriptase (TERT) gene expression and aging in mesenchymal stem cells (MSCs). The cell plates were divided into four groups including group I (control without ZnSO and ELF-EMF exposure); group II (ELF-EMF-exposure without ZnSO); group III (ZnSO treatment without ELF-EMF exposure) and group ІV (ELF-EMF exposure with ZnSO). In the presence of different concentrations of ZnSO, cells viability, TERT gene expression and percentage of senescent cells were evaluated using colorimetric assay, real-time PCR and senescence-associated β-galactosidase activity assay, respectively. In this experiment, cells were exposed to ELF-EMF for 30 min per day for 21 days in the presence and absence of ZnSO. The results revealed that ELF-EMF leads to a decrease in the expression of TERT gene and increase in the percentage of senescent cells. However, the ZnSO could significantly increase the TERT gene expression and decrease the aging of ELF-EMF-exposed MSCs. It seems that ZnSO may be a beneficial agent to delay aging of ELF-EMF-exposed MSCs due to the induction of TERT gene expression.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

mixed

Population

Rat adipose-derived mesenchymal stem cells (MSCs)

Sample size

—

Exposure

ELF · 30 min/day for 21 days

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

Rat adipose-derived MSCs exposed to 50 Hz, 20 mT ELF-EMF showed decreased TERT gene expression and increased percentage of senescent cells. Zinc sulfate treatment increased TERT gene expression and decreased aging markers in ELF-EMF-exposed MSCs.

Outcomes measured

TERT (telomerase reverse transcriptase) gene expression
Percentage of senescent cells (senescence-associated β-galactosidase activity)
Cell viability

Limitations

In vitro study (cell culture), limiting direct inference to whole organisms or humans
Sample size not reported in the provided abstract
Zinc sulfate concentrations are not specified in the provided abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "30 min/day for 21 days"
    },
    "population": "Rat adipose-derived mesenchymal stem cells (MSCs)",
    "sample_size": null,
    "outcomes": [
        "TERT (telomerase reverse transcriptase) gene expression",
        "Percentage of senescent cells (senescence-associated β-galactosidase activity)",
        "Cell viability"
    ],
    "main_findings": "Rat adipose-derived MSCs exposed to 50 Hz, 20 mT ELF-EMF showed decreased TERT gene expression and increased percentage of senescent cells. Zinc sulfate treatment increased TERT gene expression and decreased aging markers in ELF-EMF-exposed MSCs.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro study (cell culture), limiting direct inference to whole organisms or humans",
        "Sample size not reported in the provided abstract",
        "Zinc sulfate concentrations are not specified in the provided abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency electromagnetic field",
        "ELF-EMF",
        "50 Hz",
        "20 mT",
        "zinc sulfate",
        "ZnSO",
        "mesenchymal stem cells",
        "adipose-derived",
        "TERT",
        "telomerase reverse transcriptase",
        "cellular senescence",
        "β-galactosidase",
        "aging"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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