[Effects of extremely low frequency electromagnetic fields on the level of c-fos mRNA in brain and liver of mouse].

PAPER pubmed Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 2003 Animal study Effect: harm Evidence: Low

Read original paper → PMID: 14761393 Evidence Lab

Abstract

OBJECTIVE: To study the effects of extremely low frequency electromagnetic fields (ELF EMFs) on c-fos gene expression in mouse brain and liver tissues. METHODS: Mice were exposed to 50 Hz sinusoidal 0.2 mT or 6.0 mT electromagnetic field for 2 weeks or 4 weeks. Competitive RT-PCR method was used to measure c-fos mRNA level. RESULTS: After exposure to 0.2 mT or 6.0 mT field for 2 weeks, c-fos mRNA levels in brain tissue [(0.0178 +/- 0.0076) amol/120 ng cDNA and (0.0092 +/- 0.0042) amol/120 ng cDNA respectively] were higher than that of control level [(0.0012 +/- 0.0005) amol/120 ng cDNA] (P < 0.05). In liver tissue c-fos mRNA levels [(0.0117 +/- 0.0055) amol/120 ng cDNA and (0.0148 +/- 0.0162) amol/120 ng cDNA respectively] were also higher than that of control level [(0.0005 +/- 0.0005) amol/120 ng cDNA] (P < 0.05). After exposure to 0.2 mT or 6.0 mT field for 4 weeks, c-fos mRNA levels in brain tissue [(0.0100 +/- 0.0054) amol/120 ng cDNA and (0.0198 +/- 0.0079) amol/120 ng cDNA respectively] were higher than that of control level [(0.0015 +/- 0.0008) amol/120 ng cDNA] (P < 0.05). In liver tissue the exposure induced much higher expression level [(0.0173 +/- 0.0122) amol/120 ng cDNA and (0.0133 +/- 0.0090) amol/120 ng cDNA respectively] while no expression was found in the control. CONCLUSION: Exposure to 50 Hz electromagnetic fields may induce up-regulation of c-fos transcription in mouse brain and liver tissue.

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Mice

Sample size

—

Exposure

ELF · 0.05 MHz · 2 weeks or 4 weeks

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

Mice exposed to 50 Hz sinusoidal magnetic fields (0.2 mT or 6.0 mT) for 2 or 4 weeks had higher c-fos mRNA levels in brain and liver compared with controls (reported as statistically significant, P<0.05). After 4 weeks, liver tissue showed higher expression while no expression was found in controls.

Outcomes measured

c-fos mRNA level (gene expression) in brain tissue
c-fos mRNA level (gene expression) in liver tissue

Limitations

Sample size not reported in abstract
Exposure source/context (e.g., occupational/environmental setup) not described
Only one biomarker (c-fos mRNA) assessed; clinical/functional outcomes not reported
Details on randomization/blinding and animal handling not provided

Suggested hubs

occupational-exposure (0.35)
Published in an industrial hygiene/occupational diseases journal and uses 50 Hz ELF exposure relevant to occupational settings.

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": "2 weeks or 4 weeks"
    },
    "population": "Mice",
    "sample_size": null,
    "outcomes": [
        "c-fos mRNA level (gene expression) in brain tissue",
        "c-fos mRNA level (gene expression) in liver tissue"
    ],
    "main_findings": "Mice exposed to 50 Hz sinusoidal magnetic fields (0.2 mT or 6.0 mT) for 2 or 4 weeks had higher c-fos mRNA levels in brain and liver compared with controls (reported as statistically significant, P<0.05). After 4 weeks, liver tissue showed higher expression while no expression was found in controls.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "Exposure source/context (e.g., occupational/environmental setup) not described",
        "Only one biomarker (c-fos mRNA) assessed; clinical/functional outcomes not reported",
        "Details on randomization/blinding and animal handling not provided"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency",
        "ELF EMF",
        "50 Hz",
        "magnetic field",
        "mouse",
        "c-fos",
        "mRNA",
        "RT-PCR",
        "brain",
        "liver",
        "gene expression"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.34999999999999997779553950749686919152736663818359375,
            "reason": "Published in an industrial hygiene/occupational diseases journal and uses 50 Hz ELF exposure relevant to occupational settings."
        }
    ]
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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