Dose-dependent impact of extremely low-frequency electromagnetic field (ELF-EMF) on the neuroplasticity in the hippocampus of adult rats.
Research
RF Safe Research Library
Jan 1, 2026
This animal study examined whether 50 Hz ELF-EMF exposure at two intensities (1 mT vs 7 mT) alters hippocampal gene transcripts related to neuroplasticity and cell survival in adult rats across repeated exposure cycles. The authors report intensity- and exposure-number-dependent modulation of mRNA expression, with 1…
Time-Dependence Effect of 2.45 GHz RF-EMR Exposure on Male Reproductive Hormones and LHCGR
Research
RF Safe Research Library
Jan 1, 2025
This animal study exposed male Sprague Dawley rats to 2.45 GHz Wi-Fi for varying daily durations over eight weeks and assessed reproductive hormones and LHCGR expression. Serum LH and testosterone did not differ significantly from controls, but LHCGR mRNA increased with longer exposure and LHCGR protein showed…
5G Radiofrequency Exposure Reduces PRDM16 and C/EBP � mRNA Expression, Two Key Biomarkers for Brown Adipogenesis
Research
RF Safe Research Library
Jan 1, 2025
This animal study exposed juvenile and young adult Wistar rats to 5G (3.5 GHz) or 2G (900 MHz) radiofrequency fields (1.5 V/m) for 1–2 weeks and measured brown adipose tissue-related gene expression by RT-qPCR. The abstract reports significant downregulation of PRDM16 and C/EBP mRNA after 5G exposure, while…
The Extremely-Low-Frequency Electromagnetic Field Affects Apoptosis and Oxidative-Stress-Related Genes and Proteins in the Porcine Endometrium-An In Vitro Study.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed porcine endometrial slices collected during the peri-implantation period to a 50 Hz extremely-low-frequency electromagnetic field for 2 hours. The authors report decreased CASP7 and increased NOS3/eNOS mRNA and protein abundance after exposure, along with increases in additional…
P53 status, and G2/M cell cycle arrest, are determining factors in cell-death induction mediated by ELF-EMF in glioblastoma.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study tested ELF-EMF exposure in U87 and U251 glioblastoma cell lines and assessed apoptosis, cell-cycle distribution, cell size, and mRNA expression of cell-cycle genes. The authors report higher apoptosis induction and increased G2/M arrest in U87 compared with U251, alongside increased U87 cell size…
Enhancement of Fungal Enzyme Production by Radio-Frequency Electromagnetic Fields.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined whether a 2 GHz RF-EMF exposure could enhance enzyme production in a filamentous fungus. A 10-minute exposure was reported to increase extracellular total protein and α-amylase activity in culture media versus controls, alongside increased α-amylase mRNA and changes consistent with…
Effects of prolonged exposure to ELF-EMF on HERVs expression in human melanoma cells.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined whether 96-hour exposure to 50 Hz ELF-EMF affects expression of human endogenous retroviruses (HERV-H, HERV-K, HERV-W) in SK-MEL-37 human melanoma cells. Cells were continuously exposed at 1.5 or 3 mT and HERV env mRNA levels were measured by qPCR. The authors report significant…
Protein expression changes during phagocytosis influenced by low-frequency electromagnetic field exposure.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined whether a low-frequency EMF (30 mT, 7 Hz for 3 h) alters phagocytosis of latex beads and immune-related protein/gene expression in the human Mono Mac 6 macrophage cell line. The authors report that EMF exposure influenced phagocytosis-associated molecular markers, with notable changes in…
Effect of Rehabilitation with Extremely Low Frequency Electromagnetic Field on Molecular Mechanism of Apoptosis in Post-Stroke Patients.
Research
RF Safe Research Library
Jan 1, 2020
This study evaluated whether adding extremely low frequency electromagnetic field (ELF-EMF) exposure to a rehabilitation program affects apoptosis-related gene expression in post-stroke patients. Forty-eight patients (3–4 weeks post-incident) were divided into ELF-EMF and non-ELF-EMF groups, with the ELF-EMF group…
Effect of Extremely Low Frequency Electromagnetic Fields on Expression of T-bet and GATA-3 Genes and Serum Interferon-γ and Interleukin-4.
Research
RF Safe Research Library
Jan 1, 2019
This rat study examined 50 Hz ELF-EMF exposure (1–2000 μT) for 60 days and measured Th-related gene expression and cytokines in an HSA-stimulated immune model. Splenic T-bet and GATA-3 mRNA expression decreased at 1 and 100 μT. Serum IFN-γ and IL-4 were significantly decreased in the 100 μT group only before HSA…
Evaluation of the effects of extremely low frequency electromagnetic field on the levels of some inflammatory cytokines in post-stroke patients.
Research
RF Safe Research Library
Jan 1, 2019
This study evaluated whether adding therapeutic extremely low frequency electromagnetic field (ELF-EMF) treatment to a rehabilitation program affects inflammatory cytokine activity in post-stroke patients. Following ELF-EMF treatment, IL-1β plasma levels and IL-1β mRNA expression increased, and IL-2 plasma levels…
Modulation of antioxidant enzyme gene expression by extremely low frequency electromagnetic field in post-stroke patients.
Research
RF Safe Research Library
Jan 1, 2018
This study evaluated whether adding extremely low frequency electromagnetic field (ELF-EMF) therapy to standard physical therapy modulates antioxidant gene expression in post-stroke patients. Forty-eight patients were split into an ELF-EMF group and a non-ELF-EMF group, with both groups receiving the same physical…
Non-Ionizing Radiation for Cardiac Human Amniotic Mesenchymal Stromal Cell Commitment: A Physical Strategy in Regenerative Medicine.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human amniotic mesenchymal stromal cells to an ELF-EMF stimulus (7 Hz, 2.5 µT) and compared responses with a nitric oxide chemical treatment. The authors report reduced cell proliferation with no change in metabolic activity, vitality, or apoptosis after ELF-EMF exposure. They also report…
Expressions of some antioxidant genes in SH-SY5Y cells treated with β-lapachone, morphine and electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed SH-SY5Y cells to a 50 Hz, 0.50 mT electromagnetic field under intermittent or continuous conditions, alongside β-lapachone and/or morphine. The authors report condition-dependent changes in antioxidant gene expression, including decreased NQO1 and increased NQO2 under intermittent EMF…
Extremely low-frequency electromagnetic field (EMF) generates alterations in the synthesis and secretion of oestradiol-17β (E) in uterine tissues: An in vitro study.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed porcine endometrial and myometrial slices to extremely low-frequency EMF (50 or 120 Hz, 8 mT) for 2 or 4 hours, with or without progesterone. The authors report frequency- and time-dependent changes in oestradiol-17β secretion and CYP19A3 mRNA expression, with fewer changes when…
Effects of 1950 MHz radiofrequency electromagnetic fields on Aβ processing in human neuroblastoma and mouse hippocampal neuronal cells.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed HT22 mouse hippocampal neuronal cells and SH-SY5Y human neuroblastoma cells to 1950 MHz RF-EMF at SAR 6 W/kg for 2 hours per day over 3 days. RF-EMF exposure reduced mRNA levels of several Aβ-processing genes in HT22 cells, but did not change APP mRNA in SH-SY5Y cells. Protein levels of…
Different profiles of the mRNA levels of DNA repair genes in MCF-7 and SH-SY5Y cells after treatment with combination of cisplatin, 50-Hz electromagnetic field and bleomycin.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study examined whether a 50-Hz, 0.50 mT intermittent electromagnetic field modifies responses to cisplatin and bleomycin in MCF-7 and SH-SY5Y cells. The authors report increased susceptibility in MCF-7 cells with EMF added to cisplatin+bleomycin, while SH-SY5Y susceptibility was unchanged. They also…
Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy.
Research
RF Safe Research Library
Jan 1, 2017
This study reports a technology that records a specific radiofrequency electromagnetic energy signal associated with EGFR siRNA and applies it to cells. The abstract describes reductions in EGFR mRNA and protein expression versus controls, with specificity assessed using RNA microarray and antibody dot blot array. It…
Critical time delay of the pineal melatonin rhythm in humans due to weak electromagnetic exposure.
Research
RF Safe Research Library
Jan 1, 2013
This paper discusses a mechanism by which weak electromagnetic exposure could perturb radical pair recombination rates, potentially increasing radical lifetimes. It reports significant melatonin interruption and circadian rhythm changes due to perturbation of chemical reaction rates, and notes that mRNA degradation…
Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2013
This in vitro study examined whether extremely low frequency electromagnetic field (ELF-EMF) exposure alters MMP-2 expression in cultured human fetal scleral fibroblasts and whether puerarin modifies this response. The authors report increased MMP-2 mRNA and protein expression after 0.2 mT ELF-EMF exposure for 24…
[Effects of static magnetic field at different times on the proliferation and differentiation of osteoblasts in vitro].
Research
RF Safe Research Library
Jan 1, 2012
This in vitro study exposed newborn rat calvarial osteoblasts to a 3.9 mT static magnetic field for varying durations (0–4 hours). The authors report increased cell proliferation and markers of osteoblast differentiation and mineralization versus control, including higher ALP activity, calcium content, and…
Down-regulation of metallothionein 1 and 2 after exposure to electromagnetic field in mouse testis.
Research
RF Safe Research Library
Jan 1, 2011
This animal study examined mouse testis after 8 weeks of exposure to a 3 MT electromagnetic field (4 hours/day). The authors report significant histological changes (increased primary spermatocytes, increased Leydig cells, and increased basement membrane thickness) in exposed mice versus controls. MT1 and MT2…