Analysis of DPPH inhibition and structure change of corn peptides treated by pulsed electric field technology.
Abstract
In this study, the effects on antioxidant activity and structure change of corn peptides (CPS) with 10 to 30 kDa molecular weight (MW) treated by pulsed electric field (PEF) technology were investigated. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) inhibition was used to evaluate the antioxidant activity of CPS. Response surface methodology (RSM) was used to investigate the effects of PEF treatment parameters on antioxidant activity of CPS. The optimal conditions were as follows: concentration of CPS 10 mg mL(-1), electric field intensity 15 kV cm(-1), and pulse frequency 2,000 Hz. Under the optimized conditions, the DPPH inhibition of CPS increased 32.1 %, compared to the sample untreated. And mid-infrared spectroscopy (MIR) was used for analyzing the structure change of CPS. The results showed that PEF technology could obviously increase the DPPH inhibition of CPS under the optimized conditions (P < 0.05).
AI evidence extraction
Main findings
Pulsed electric field (PEF) treatment parameters were optimized (CPS concentration 10 mg/mL, electric field intensity 15 kV/cm, pulse frequency 2,000 Hz). Under optimized conditions, DPPH inhibition of corn peptides increased by 32.1% compared with untreated samples, and MIR analysis was used to assess structural changes.
Outcomes measured
- DPPH inhibition (antioxidant activity) of corn peptides
- Structural change of corn peptides (mid-infrared spectroscopy)
Limitations
- No sample size reported in abstract
- No exposure duration reported in abstract
- Study is on food peptides (not human/animal health outcomes)
View raw extracted JSON
{
"study_type": "other",
"exposure": {
"band": null,
"source": "pulsed electric field (PEF) technology",
"frequency_mhz": null,
"sar_wkg": null,
"duration": null
},
"population": null,
"sample_size": null,
"outcomes": [
"DPPH inhibition (antioxidant activity) of corn peptides",
"Structural change of corn peptides (mid-infrared spectroscopy)"
],
"main_findings": "Pulsed electric field (PEF) treatment parameters were optimized (CPS concentration 10 mg/mL, electric field intensity 15 kV/cm, pulse frequency 2,000 Hz). Under optimized conditions, DPPH inhibition of corn peptides increased by 32.1% compared with untreated samples, and MIR analysis was used to assess structural changes.",
"effect_direction": "benefit",
"limitations": [
"No sample size reported in abstract",
"No exposure duration reported in abstract",
"Study is on food peptides (not human/animal health outcomes)"
],
"evidence_strength": "low",
"confidence": 0.7399999999999999911182158029987476766109466552734375,
"peer_reviewed_likely": "yes",
"keywords": [
"pulsed electric field",
"PEF",
"corn peptides",
"DPPH inhibition",
"antioxidant activity",
"response surface methodology",
"mid-infrared spectroscopy"
],
"suggested_hubs": []
}
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