Power frequency magnetic field promotes a more malignant phenotype in neuroblastoma cells via redox- related mechanisms

Abstract

Power frequency magnetic field promotes a more malignant phenotype in neuroblastoma cells via redox- related mechanisms Falone S, Santini S Jr, Cordone V, Cesare P, Bonfigli A, Grannonico M, Di Emidio G, Tatone C, Cacchio M, Amicarelli F. Power frequency magnetic field promotes a more malignant phenotype in neuroblastoma cells via redox-related mechanisms.Sci Rep. 2017 Sep 13;7(1):11470. doi: 10.1038/s41598-017-11869-8. Abstract In accordance with the classification of the International Agency for Research on Cancer, extremely low frequency magnetic fields (ELF-MF) are suspected to promote malignant progression by providing survival advantage to cancer cells through the activation of critical cytoprotective pathways. Among these, the major antioxidative and detoxification defence systems might be targeted by ELF-MF by conferring cells significant resistance against clinically-relevant cytotoxic agents. We investigated whether the hyperproliferation that is induced in SH-SY5Y human neuroblastoma cells by a 50 Hz, 1 mT ELF magnetic field was supported by improved defence towards reactive oxygen species (ROS) and xenobiotics, as well as by reduced vulnerability against both H2O2 and anti-tumor ROS-generating drug doxorubicin. ELF-MF induced a proliferative and survival advantage by activating key redox-responsive antioxidative and detoxification cytoprotective pathways that are associated with a more aggressive behavior of neuroblastoma cells. This was coupled with the upregulation of the major sirtuins, as well as with increased signaling activity of the erythroid 2-related nuclear transcription factor 2 (NRF2). Interestingly, we also showed that the exposure to 50 Hz MF as low as 100 µT may still be able to alter behavior and responses of cancer cells to clinically-relevant drugs. ncbi.nlm.nih.gov

AI evidence extraction

Main findings

In SH-SY5Y human neuroblastoma cells, exposure to a 50 Hz, 1 mT extremely low frequency magnetic field induced hyperproliferation and a proliferative/survival advantage. The abstract reports activation of redox-responsive antioxidative and detoxification cytoprotective pathways (including increased NRF2 signaling and upregulation of sirtuins), reduced vulnerability to H2O2 and doxorubicin, and notes that effects on behavior and drug responses were also observed at 50 Hz as low as 100 µT.

Outcomes measured

• Cell proliferation/hyperproliferation
• Cell survival advantage
• Resistance/vulnerability to oxidative stress (H2O2)
• Response to doxorubicin (ROS-generating anti-tumor drug)
• Activation of redox-responsive antioxidative and detoxification pathways
• Upregulation of sirtuins
• NRF2 signaling activity
• Reactive oxygen species (ROS) defense

Limitations

• In vitro cell culture model; findings may not translate to humans or in vivo tumors
• Exposure duration not reported in the provided abstract
• Sample size/replication details not reported in the provided abstract
• Mechanistic claims are based on pathway/signaling observations as described in the abstract; full methods/results not provided here

Suggested hubs

• occupational-exposure (0.35)
  Study concerns power-frequency (50 Hz) magnetic field exposure, relevant to ELF/power-line and occupational contexts, though the model is in vitro.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": null
    },
    "population": "SH-SY5Y human neuroblastoma cells",
    "sample_size": null,
    "outcomes": [
        "Cell proliferation/hyperproliferation",
        "Cell survival advantage",
        "Resistance/vulnerability to oxidative stress (H2O2)",
        "Response to doxorubicin (ROS-generating anti-tumor drug)",
        "Activation of redox-responsive antioxidative and detoxification pathways",
        "Upregulation of sirtuins",
        "NRF2 signaling activity",
        "Reactive oxygen species (ROS) defense"
    ],
    "main_findings": "In SH-SY5Y human neuroblastoma cells, exposure to a 50 Hz, 1 mT extremely low frequency magnetic field induced hyperproliferation and a proliferative/survival advantage. The abstract reports activation of redox-responsive antioxidative and detoxification cytoprotective pathways (including increased NRF2 signaling and upregulation of sirtuins), reduced vulnerability to H2O2 and doxorubicin, and notes that effects on behavior and drug responses were also observed at 50 Hz as low as 100 µT.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cell culture model; findings may not translate to humans or in vivo tumors",
        "Exposure duration not reported in the provided abstract",
        "Sample size/replication details not reported in the provided abstract",
        "Mechanistic claims are based on pathway/signaling observations as described in the abstract; full methods/results not provided here"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency magnetic field",
        "ELF-MF",
        "power frequency",
        "50 Hz",
        "1 mT",
        "100 µT",
        "neuroblastoma",
        "SH-SY5Y",
        "malignant phenotype",
        "proliferation",
        "survival",
        "redox",
        "ROS",
        "NRF2",
        "sirtuins",
        "doxorubicin",
        "H2O2"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.34999999999999997779553950749686919152736663818359375,
            "reason": "Study concerns power-frequency (50 Hz) magnetic field exposure, relevant to ELF/power-line and occupational contexts, though the model is in vitro."
        }
    ]
}

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