Selenium supplementation ameliorates electromagnetic field-induced oxidative stress in HEK293 cells

Abstract

Selenium supplementation ameliorates electromagnetic field-induced oxidative stress in HEK293 cells Özsobacı NP, Ergün DD, Durmuş S, Tunçdemir M, Uzun H, Gelişgen R, Özçelik D.Selenium supplementation ameliorates electromagnetic field-induced oxidative stress in the HEK293 cells. Journal of Trace Elements in Medicine and Biology. Available online 13 April 2018 There is a widespread use of 2.4 GHz electromagnetic radiation emitting devices especially in communication and education. Recent studies show the adverse effects of electromagnetic fields (EMF) such as oxidative stress, cellular damage and apoptosis on tissues. Selenium (Se) has an antioxidant properties by inhibiting oxidative damage being within the structure of antioxidant enzymes like glutathione peroxidase (GSH-Px) and it has also regulatory function for cell cycle and apoptosis. The aim of this study was to investigate the effect of Se on 2.4 GHz frequency EMF exposed human embryonic kidney cells (HEK293) by means of alterations in apoptotic and oxidative stress parameters. Our study was planned as control, EMF, 100 nM Se+EMF, 200 nM Se+EMF groups. EMF groups were exposed to 2.4 GHz EMF for 1 hour, element groups were incubated with two different doses of Se added cell culture medium for 48 hour before EMF exposure. MDA levels were significantly higher whereas SOD and GSH-Px activities were significantly lower in EMF compared to control. 100 and 200 nM Se + EMF application decreased MDA levels, increased SOD and GSH-Px activities than EMF. Apoptosis and caspase-3 were statistically significantly higher but bcl-2 was lower in EMF than control. Apoptosis and caspase-3 were lower in 100 and 200 nM Se + EMF, although bcl-2 were higher than EMF. In conclusion, Se has protective effects against 2.4 GHz EMF-induced oxidative stress by reducing lipid peroxidation, regulating SOD and GSH-Px activity. Also, Se has inhibitory effect on 2.4 GHz EMF induced apoptosis by increasing the expression of anti-apoptotic protein bcl-2 and suppressing apoptosis regulatory protein caspase-3. sciencedirect.com 00e4a6edfba&pid=1-s2.0-S0946672X17309768-main.pdf

AI evidence extraction

Main findings

Compared with control, 2.4 GHz EMF exposure increased MDA and increased apoptosis/caspase-3 while decreasing SOD and GSH-Px activities and lowering bcl-2. Selenium (100 or 200 nM) pre-treatment before EMF exposure decreased MDA and apoptosis/caspase-3 and increased SOD, GSH-Px, and bcl-2 relative to EMF alone.

Outcomes measured

• MDA (lipid peroxidation)
• SOD activity
• GSH-Px activity
• Apoptosis
• Caspase-3
• bcl-2

Limitations

• No sample size reported in abstract
• SAR/dosimetry not reported in abstract
• Exposure setup/source details not reported in abstract

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "RF",
        "source": null,
        "frequency_mhz": 2400,
        "sar_wkg": null,
        "duration": "1 hour (EMF exposure); selenium pre-incubation 48 hour before exposure"
    },
    "population": "HEK293 (human embryonic kidney) cells",
    "sample_size": null,
    "outcomes": [
        "MDA (lipid peroxidation)",
        "SOD activity",
        "GSH-Px activity",
        "Apoptosis",
        "Caspase-3",
        "bcl-2"
    ],
    "main_findings": "Compared with control, 2.4 GHz EMF exposure increased MDA and increased apoptosis/caspase-3 while decreasing SOD and GSH-Px activities and lowering bcl-2. Selenium (100 or 200 nM) pre-treatment before EMF exposure decreased MDA and apoptosis/caspase-3 and increased SOD, GSH-Px, and bcl-2 relative to EMF alone.",
    "effect_direction": "mixed",
    "limitations": [
        "No sample size reported in abstract",
        "SAR/dosimetry not reported in abstract",
        "Exposure setup/source details not reported in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "2.4 GHz",
        "electromagnetic field",
        "RF",
        "HEK293",
        "selenium",
        "oxidative stress",
        "apoptosis",
        "MDA",
        "SOD",
        "GSH-Px",
        "caspase-3",
        "bcl-2"
    ],
    "suggested_hubs": []
}

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