900 MHz GSM Cell Phone Radiation Alters Human MCF-7 Cells & Stem Cells

PAPER manual 2018 In vitro study Effect: harm Evidence: Low

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Abstract

900 MHz GSM Cell Phone Radiation Alters Human MCF-7 Cells & Stem Cells Shahbazi-Gahrouei D, Hashemi-Beni B, Moradi A, Aliakbari M, Shahbazi-Gahrouei S. Exposure to Global System for Mobile Communication 900 MHz Cellular Phone Radiofrequency Alters Growth, Proliferation and Morphology of Michigan Cancer Foundation-7 Cells and Mesenchymal Stem Cells. Int J Prev Med. 2018 Jun 19;9:51. Abstract Background: Today, using cellular phone and its harmful effects in human life is growing. The aim of this study is to investigate the effect of the global system for mobile communication (GSM) 900 MHz cellular phone radiofrequency waves on growth, morphology, and proliferation rate of mesenchymal stem cells and Michigan Cancer Foundation (MCF-7) cells within the specific distance and intensity. Methods: MCF-7 and human adipose-derived stem cells (HADSCs) were exposed to GSM cellular phones 900 MHz frequency with intensity of 354.6 μW/cm2 during different exposure times 6, 21, 51, and 101 min/day with an interval of 10 min for each subsequent radiation exposure for 3 and 5 days at 10 and 20 cm distances from antenna. 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide assay and trypan blue test were used to determine the growth of cells and cell viability, respectively. Statistical analyses were carried out using three- way ANOVA. Differences were significant when P < 0.05. Results: The proliferation rates of both MCF-7 and HADSCs cells in all exposure groups were significantly lower than controls (P < 0.05). There was a significant effect on the percentage of cell survival with increase the period of time from 3 to 5 days for MCF-7 (P < 0.01) and HADSCs (P = 0.02), respectively. Variations in distance had no significant effect on the percentage of cell survival (P = 0.35) on MCF-7 (P = 0.02) and HADSCs (P = 0.09) cells, respectively. Conclusions: The results showed that radiation of GSM 900 MHz cellular phone may be reduced cell viability and proliferation rates of both cells. It is recommended to reduce exposure time, increase distance from antenna, and reserve the use of cell phones for shorter conversations to prevent its biological and harmful effects. Further studies with other intensities and frequencies on different cells are recommended. Open access paper: ncbi.nlm.nih.gov

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

harm

Population

—

Sample size

—

Exposure

RF mobile phone · 900 MHz · 6, 21, 51, or 101 min/day (with 10 min interval between subsequent exposures) for 3 or 5 days; distances 10 or 20 cm; intensity 354.6 μW/cm2

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

MCF-7 cells and human adipose-derived stem cells exposed to 900 MHz GSM phone radiation (354.6 μW/cm2) showed significantly lower proliferation rates than controls across all exposure groups (P < 0.05). Cell survival/viability changed significantly when exposure duration increased from 3 to 5 days for both cell types, while distance (10 vs 20 cm) was reported as not significantly affecting cell survival.

Outcomes measured

Cell growth (MTT assay)
Cell viability/survival (trypan blue test)
Cell proliferation rate
Cell morphology

Limitations

Sample size not reported in abstract
SAR not reported; exposure characterized by power density and distance only
In vitro cell model; generalizability to humans not established
Details of exposure setup/dosimetry beyond frequency, intensity, distance, and timing not provided in abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "RF",
        "source": "mobile phone",
        "frequency_mhz": 900,
        "sar_wkg": null,
        "duration": "6, 21, 51, or 101 min/day (with 10 min interval between subsequent exposures) for 3 or 5 days; distances 10 or 20 cm; intensity 354.6 μW/cm2"
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Cell growth (MTT assay)",
        "Cell viability/survival (trypan blue test)",
        "Cell proliferation rate",
        "Cell morphology"
    ],
    "main_findings": "MCF-7 cells and human adipose-derived stem cells exposed to 900 MHz GSM phone radiation (354.6 μW/cm2) showed significantly lower proliferation rates than controls across all exposure groups (P < 0.05). Cell survival/viability changed significantly when exposure duration increased from 3 to 5 days for both cell types, while distance (10 vs 20 cm) was reported as not significantly affecting cell survival.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "SAR not reported; exposure characterized by power density and distance only",
        "In vitro cell model; generalizability to humans not established",
        "Details of exposure setup/dosimetry beyond frequency, intensity, distance, and timing not provided in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "GSM",
        "900 MHz",
        "cell phone radiation",
        "radiofrequency",
        "MCF-7",
        "mesenchymal stem cells",
        "human adipose-derived stem cells",
        "cell viability",
        "proliferation",
        "morphology",
        "MTT",
        "trypan blue"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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