Evaluation of frequency magnetic field, static field, and Temozolomide on viability, free radical production and gene expression (p53) in the human glioblastoma cell line (A172)
Abstract
Evaluation of frequency magnetic field, static field, and Temozolomide on viability, free radical production and gene expression (p53) in the human glioblastoma cell line (A172) Ahmad Ashta, Gholamreza Motalleb, Meysam Ahmadi-Zeidabadi. Evaluation of frequency magnetic field, static field, and Temozolomide on viability, free radical production and gene expression (p53) in the human glioblastoma cell line (A172). Electromagn Biol Med. 2020 Jul 15;1-12. doi: 10.1080/15368378.2020.1793171. Abstract Thirteen million cancer deaths and 21.7 million new cancer cases are expected in the world by 2030. Glioblastoma is the most common primary malignant tumor of the central nervous system which is the most lethal type of primary brain tumor in adults with the survival time of 12-15 months after the initial diagnosis. Glioblastoma is the most common and most malignant type of brain tumor, and despite surgery, chemotherapy and radiation treatment, the average survival of patients is about 14 months. The current research showed that the frequency magnetic field (FMF) and static magnetic field (SMF) can influence cancer cell proliferation and coupled with anticancer drugs may provide a new strategy for cancer therapy. At the present study, we investigated the effects of FMF (10 Hz, 50 G), SMF (50 G) and Temozolomide (200 μm) on viability, free radical production, and p53 followed by p53 protein expression in the human glioblastoma cell line (A172) by MTT, NBT, RT-PCR and Western blot. Results showed that the effect of Temozolomide (TMZ) with SMF and FMF together increased the cytotoxicity, free radical production, and p53 followed by p53 protein expression in the human glioblastoma cell line (A172). pubmed.ncbi.nlm.nih.gov
AI evidence extraction
Main findings
In A172 human glioblastoma cells, temozolomide combined with static magnetic field (50 G) and frequency magnetic field (10 Hz, 50 G) reportedly increased cytotoxicity, free radical production, and p53 gene and protein expression compared with temozolomide alone.
Outcomes measured
- Cell viability (MTT)
- Free radical production (NBT)
- p53 gene expression (RT-PCR)
- p53 protein expression (Western blot)
Limitations
- In vitro cell-line study; findings may not translate to humans or clinical outcomes.
- Exposure duration and detailed experimental conditions are not provided in the abstract.
- Sample size and replication details are not reported in the abstract.
- Comparative effects of FMF or SMF alone versus control are not clearly described in the abstract.
View raw extracted JSON
{
"publication_year": 2020,
"study_type": "in_vitro",
"exposure": {
"band": "ELF",
"source": "laboratory magnetic field exposure",
"frequency_mhz": null,
"sar_wkg": null,
"duration": null
},
"population": "Human glioblastoma cell line (A172)",
"sample_size": null,
"outcomes": [
"Cell viability (MTT)",
"Free radical production (NBT)",
"p53 gene expression (RT-PCR)",
"p53 protein expression (Western blot)"
],
"main_findings": "In A172 human glioblastoma cells, temozolomide combined with static magnetic field (50 G) and frequency magnetic field (10 Hz, 50 G) reportedly increased cytotoxicity, free radical production, and p53 gene and protein expression compared with temozolomide alone.",
"effect_direction": "harm",
"limitations": [
"In vitro cell-line study; findings may not translate to humans or clinical outcomes.",
"Exposure duration and detailed experimental conditions are not provided in the abstract.",
"Sample size and replication details are not reported in the abstract.",
"Comparative effects of FMF or SMF alone versus control are not clearly described in the abstract."
],
"evidence_strength": "low",
"confidence": 0.7399999999999999911182158029987476766109466552734375,
"peer_reviewed_likely": "yes",
"stance": "concern",
"stance_confidence": 0.59999999999999997779553950749686919152736663818359375,
"summary": "This in vitro study examined whether a frequency magnetic field (10 Hz, 50 G) and a static magnetic field (50 G), alone or with temozolomide (200 μm), affect viability, free radical production, and p53 expression in A172 human glioblastoma cells. The authors report that temozolomide combined with both magnetic field exposures increased cytotoxicity, free radical production, and p53 gene and protein expression. The abstract does not provide sample size, exposure duration, or detailed comparisons for magnetic fields alone.",
"key_points": [
"The study used the A172 human glioblastoma cell line and assessed viability, oxidative/free radical markers, and p53 expression.",
"Exposures included a frequency magnetic field at 10 Hz and 50 G and a static magnetic field at 50 G.",
"Temozolomide was tested at 200 μm, including in combination with the magnetic field exposures.",
"The authors report increased cytotoxicity when temozolomide was combined with both SMF and FMF.",
"The combination condition was also reported to increase free radical production.",
"p53 gene expression and p53 protein expression were reported to increase in the combination condition.",
"Key methodological details (e.g., exposure duration, sample size) are not stated in the abstract."
],
"categories": [
"In Vitro",
"ELF Magnetic Fields",
"Cancer Biology",
"Oxidative Stress",
"Gene Expression"
],
"tags": [
"Glioblastoma",
"A172 Cells",
"Static Magnetic Field",
"Extremely Low Frequency Magnetic Field",
"10 Hz",
"50 Gauss",
"Temozolomide",
"Cell Viability",
"Cytotoxicity",
"Free Radical Production",
"Oxidative Stress",
"p53",
"RT-PCR",
"Western Blot",
"MTT Assay"
],
"keywords": [
"glioblastoma",
"A172",
"static magnetic field",
"frequency magnetic field",
"ELF",
"10 Hz",
"50 G",
"temozolomide",
"cell viability",
"free radicals",
"p53",
"RT-PCR"
],
"suggested_hubs": [],
"social": {
"tweet": "In an in vitro study of A172 glioblastoma cells, temozolomide combined with a 50 G static magnetic field and a 10 Hz, 50 G magnetic field was reported to increase cytotoxicity, free radical production, and p53 gene/protein expression.",
"facebook": "A laboratory study in A172 human glioblastoma cells reported that combining temozolomide with static (50 G) and low-frequency (10 Hz, 50 G) magnetic fields increased cytotoxicity, free radical production, and p53 gene and protein expression. Details such as exposure duration and sample size were not provided in the abstract.",
"linkedin": "This in vitro paper reports that temozolomide (200 μm) combined with a 50 G static magnetic field and a 10 Hz, 50 G frequency magnetic field increased cytotoxicity, free radical production, and p53 gene/protein expression in A172 glioblastoma cells, based on MTT/NBT/RT-PCR/Western blot assays."
}
}
AI can be wrong. Always verify against the paper.
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