Changes in miR-21, -138, -141, -135 expression and apoptosis/necrosis levels in HCT116 colon cancer cells under 50 Hz ELF-EMF exposure at 0.4 and 0.8 mT
This in vitro study exposed HCT116 colorectal carcinoma cells to 50 Hz ELF-EMF at 0.4 or 0.8 mT for 9 or 18 hours under continuous or discontinuous conditions. The authors report significant changes in miR-21, miR-141, miR-135, and miR-138 expression and increased apoptosis and necrosis measured by flow cytometry. They interpret some microRNA changes as potentially tumor-suppressive under their exposure conditions, while noting miR-138 downregulation as potentially unfavorable.
Key points
- HCT116 colon cancer cells were exposed to 50 Hz ELF-EMF with varying intensity, duration, and continuity, creating eight exposure groups plus controls.
- Apoptosis and necrosis were assessed using flow cytometry across exposure conditions.
- Expression of miR-21, miR-141, miR-135, and miR-138 was measured using RT-qPCR.
- The abstract reports significant alterations in these microRNAs under ELF-EMF exposure.
- The authors describe downregulation of miR-135 and miR-21 and upregulation of miR-141 as potentially supportive of tumor growth control in this model.
- Downregulation of miR-138 is described as a potentially negative factor.
- Apoptosis and necrosis rates increased under the applied ELF-EMF conditions.
Referenced studies & papers
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AI-generated summaries may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.
AI-generated summaries may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.
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