Archive

This review synthesizes evidence on how direct current and alternating current electrical stimulation affect neurons and glia in the central nervous system, spanning basic research and clinical translation. It reports polarity- and parameter-dependent effects on axonal growth, along with immunomodulatory glial…

This in vitro study examined extremely low-frequency electromagnetic field (ELF-EMF) effects on spinal cord-derived neural stem cells (NSCs) from adult mice. The authors report that ELF-EMFs increase NSC proliferation and self-renewal with upregulation of Sox2. They also report activation of T-type calcium channels,…

This in vitro study tested whether daily exposure to a 10 mT ELF electromagnetic field, combined with miR-16 and miR-451 transfection, could enhance trans-differentiation of human dermal fibroblasts toward erythroid-like progenitors. The authors report increased erythroid-like differentiation with ELF-EMF exposure,…

This in vitro study examined how electromagnetic field (EMF) coupling parameters and field direction interact with 2D versus 3D culture environments to influence adipose-derived stem cell (ADSC) proliferation and osteogenic/chondrogenic differentiation. The abstract reports different “optimal” magnetic flux densities…

This in vitro study tested whether ELF-EMF exposure (50 Hz, 1 mT; 30 min/day) influences osteogenic differentiation markers in rat bone-marrow-derived stem cells cultured in osteogenic medium or in human adipose-stem cell-conditioned medium. The OM + EMF condition showed higher ALP activity and higher Runx2 and Ocn…

This in vitro study exposed dental pulp mesenchymal stem cells to 50 Hz pulsed electromagnetic fields at 0.5 or 1 mT for 20 or 40 minutes per day over seven days. Cell survival/proliferation was generally higher than control across exposure groups, except for the 40 minutes/day at 1 mT condition. DMP1 expression was…

This in vitro study examined intermittent extremely low-frequency electromagnetic field (ELF-EMF) exposure (6 h/day) in human periodontal ligament mesenchymal stem cells during osteogenic differentiation. After 10 days, ELF-EMF-exposed cells showed increased proliferation and calcium deposition versus sham.…

This in vitro study exposed human adipose-derived mesenchymal stem progenitor cells to an alternating EMF generated by the IteraCoil device at 0.05 and 1 kHz. The authors report that EMF exposure was associated with osteoblastic differentiation in the absence of exogenous pro-osteogenic supplements, assessed by…

This animal and in vitro study evaluated a composite osteochondral scaffold with bone marrow mesenchymal stem cells (BMSCs) combined with low-frequency electromagnetic field (EMF) stimulation. The authors report that EMF promoted BMSC proliferation and chondrogenic differentiation in vitro, partly via PI3K/AKT/mTOR…

This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…

This in vitro and rat-model study evaluated sinusoidal low-frequency EMF combined with a PCL/nHA scaffold seeded with BMSCs for intervertebral fusion. The authors report enhanced osteogenic differentiation of BMSCs and pro-osteogenic effects of conditioned medium from EMF-treated BMSCs, with suggested involvement of…

This animal and in vitro study evaluated electromagnetic fields (EMF) treatment combined with a 3D-printed PLA/HA scaffold seeded with rat bone marrow mesenchymal stem cells (BMSCs) for cranial defect repair. The authors report that EMF enhanced BMSC proliferation and osteogenic differentiation and was partly…

This in vitro study evaluated whether extremely low-frequency EMF (50 Hz,

This in vitro study developed carboxymethyl chitosan (CMC)-grafted polycaprolactone (PCL) nanofiber scaffolds for bone tissue engineering. It reports mineralization and osteonectin expression in adipose mesenchymal stem cells cultured on the scaffolds, suggesting osteoinductive properties of the grafted CMC. The…

This in vitro study compared commonly used pulsed electromagnetic field (PEMF) exposures with electromagnetic fields tuned to satisfy the ion cyclotron resonance (ICR) condition for calcium. After 3 weeks, the calcium ICR condition was associated with increased glycosaminoglycans and type II collagen synthesis and…

This in vitro study exposed human amniotic mesenchymal stromal cells to an ELF-EMF stimulus (7 Hz, 2.5 µT) and compared responses with a nitric oxide chemical treatment. The authors report reduced cell proliferation with no change in metabolic activity, vitality, or apoptosis after ELF-EMF exposure. They also report…

This animal study examined whether electromagnetic field (EMF) pretreatment has lasting (“legacy”) effects on rat bone marrow mesenchymal stem cells (BMSCs) and whether EMF-pretreated BMSC sheets improve fracture nonunion healing in rats. The authors report increased BMSC proliferation and enhanced…

This in vitro study examined whether 50 Hz, 2 mT extremely low-frequency electromagnetic fields affect ATRA-driven granulocytic differentiation in NB4 acute promyelocytic leukemia cells. The authors report reduced proliferation and enhanced maturation/differentiation markers with ELF-EMF exposure. They also report…

This in vitro study examined how 50 Hz low-frequency EMF and a nitric oxide donor (Deta-NO) affect stemness and neuronal differentiation markers in rat bone marrow mesenchymal stem cells. The authors report that high NO (1 mM) combined with EMF reduced stemness markers and increased neuronal differentiation markers,…