Conformational changes of β-thalassemia major hemoglobin and oxidative status of plasma after in vitro exposure to extremely low-frequency electromagnetic fields: An artificial neural network analysis.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed blood samples from β-thalassemia major patients and matched healthy donors (n=12 each) to 50 Hz ELF-EMF at 0.5 and 1 mT for 1–2 hours. The authors report that two-hour exposure induced significant oxidative changes in β-thalassemia major samples and examined protein/Hb structural features…
Pulsed and Discontinuous Electromagnetic Field Exposure Decreases Temozolomide Resistance in Glioblastoma by Modulating the Expression of O-Methylguanine-DNA Methyltransferase, Cyclin-D1, and p53.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study evaluated 50 Hz, 70 G electromagnetic field exposure in combination with temozolomide (TMZ) in T98 and A172 glioblastoma cell lines. The authors report that EMF plus TMZ increased cell death and reduced migration potential compared with controls. They also report increased p53 gene expression and…
Translocation of silica nanospheres through giant unilamellar vesicles (GUVs) induced by a high frequency electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study used giant unilamellar vesicles (GUVs) to examine effects of an 18 GHz electromagnetic field on membrane behavior. The authors report increased membrane permeability after exposure, inferred from internalisation of 23.5 nm hydrophilic silica nanospheres. Complementary molecular dynamics…
First Identification of the Effects of Low Frequency Electromagnetic Field on the Micromolecular Changes in Adipose Tissue-Derived Mesenchymal Stem Cells by Fourier Transform Infrared Spectroscopy.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed adipose tissue-derived mesenchymal stem cells to a continuous 50 Hz electromagnetic field at 1.5 mT for 24, 48, or 72 hours and assessed micromolecular changes using FTIR spectroscopy. The authors report the largest FTIR spectral differences versus control in nucleic acids and proteins…
EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined whether extremely low-frequency electromagnetic fields (ELF-EMF) at 0.2 or 2 mT (continuous or 1.5 h on/1.5 h off) for 18 hours alter viability and gene/miRNA expression in AGS gastric cancer cells. The authors report a significant reduction in cell viability at 2 mT continuous exposure.…
Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits.
Research
RF Safe Research Library
Jan 1, 2021
This animal and in vitro study evaluated a composite osteochondral scaffold with bone marrow mesenchymal stem cells (BMSCs) combined with low-frequency electromagnetic field (EMF) stimulation. The authors report that EMF promoted BMSC proliferation and chondrogenic differentiation in vitro, partly via PI3K/AKT/mTOR…
The Dynamic(s) of Adipose Stem Cell System, Their Survival, and Cessation under the Influence of Electromagnetic Fields.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined how extremely low frequency EMF affects adipose-derived stem cell proliferation, cell cycle, viability, and death using fluorescent microscopy and flow cytometry. Under continuous exposure at 76.6 Hz and 20 T (ICR-based parameters), the authors report a statistically significant increase…
Effects of Radiofrequency Electromagnetic Field (RF-EMF) exposure on male fertility and pregnancy and birth outcomes: Protocols for a systematic review of experimental studies in non-human mammals and in human sperm exposed in vitro.
Research
RF Safe Research Library
Jan 1, 2021
This paper presents protocols for a systematic review of experimental studies assessing RF-EMF exposure (100 kHz–300 GHz) and outcomes related to male fertility and pregnancy/birth effects. The review will cover three evidence streams: non-human mammal male reproductive outcomes, human sperm exposed in vitro, and in…
The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed mouse spermatogonial stem cells to 50 Hz EMF (2.5 mT; 1 h/day for 5 days) and assessed oxidative stress and cell health outcomes. The authors report increased ROS and apoptosis with reduced antioxidant (catalase) expression, viability, and colonization after EMF exposure. Sertoli…
Exposure to long-term evolution radiofrequency electromagnetic fields decreases neuroblastoma cell proliferation via Akt/mTOR-mediated cellular senescence.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined LTE RF-EMF exposure effects on SH-SY5Y neuroblastoma cells. The authors report reduced cell proliferation and a G0/G1 cell-cycle delay after 1760 MHz exposure at 4 W/kg for 4 hr/day over 4 days. They report no marked DNA damage signal (γH2AX) and no apoptosis activation, but increased…
Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed developing pre-B lymphocytes undergoing V(D)J recombination to low-intensity RF EMFs for 48 hours across 720–1224 MHz. The authors report a frequency- and dose-dependent decrease in recombination, with stronger reductions around 950–1000 MHz at relatively low power densities. γH2AX levels…
Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…
Necroptosis triggered by ROS accumulation and Ca overload, partly explains the inflammatory responses and anti-cancer effects associated with 1Hz, 100 mT ELF-MF in vivo.
Research
RF Safe Research Library
Jan 1, 2021
This animal and in vitro study examined ELF magnetic field exposure in a mouse breast tumor model and a breast cancer cell line. The authors report tumor growth suppression and reduced expression of Ki-67, CD31, VEGFR2, and MMP-9 after exposure. They also report increased IFN-γ and TNF-α and increased…
Sinusoidal electromagnetic fields accelerate bone regeneration by boosting the multifunctionality of bone marrow mesenchymal stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro and rat study evaluated sinusoidal electromagnetic field (EMF) treatment of bone marrow mesenchymal stem cells (BMSCs) seeded on 3D-printed scaffolds for repairing critical-sized calvarial defects. The authors report that EMF-treated cell scaffolds accelerated bone defect repair in rats.…
Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed HaCaT human keratinocytes to 1760 MHz RF-EMF (SAR 4.0 W/kg; 2 h/day for 4 days). The authors report increased intracellular ROS and increased expression/activity of several matrix metalloproteinases, along with increased phosphorylation of FoxO3a and ERK1/2. Cell growth/viability and…
Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…
Effect of extremely low frequency electromagnetic field parameters on the proliferation of human breast cancer.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study tested how varying ELF-EMF parameters (frequency, flux density, duration, and continuous vs switching exposure) affect breast cancer and normal breast epithelial cell lines. The authors report that an optimized switching exposure pattern (7.83 ± 0.3 Hz, 1 mT) reduced viability of MDA-MB-231 cells…
Low-frequency electromagnetic fields combined with tissue engineering techniques accelerate intervertebral fusion.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro and rat-model study evaluated sinusoidal low-frequency EMF combined with a PCL/nHA scaffold seeded with BMSCs for intervertebral fusion. The authors report enhanced osteogenic differentiation of BMSCs and pro-osteogenic effects of conditioned medium from EMF-treated BMSCs, with suggested involvement of…
Low-frequency electromagnetic field influences human oral mucosa keratinocyte viability in response to lipopolysaccharide or minocycline treatment in cell culture conditions.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined whether low-frequency electromagnetic field (LF-EMF) exposure affects viability of human oral mucosa keratinocytes. Apoptosis (early/late) and necrosis were measured by flow cytometry, including conditions with lipopolysaccharide (LPS) or minocycline. The authors report that LF-EMF, alone…
Synergistic effect of electromagnetic fields and nanomagnetic particles on osteogenesis through calcium channels and p-ERK signaling.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro and animal study evaluated whether extremely low frequency EMF exposure (1 mT, 45 Hz, 8 h/day) combined with Fe-based nanomagnetic particles enhances osteogenesis. The authors report no detectable cytotoxicity and increased osteogenic markers/proteins with combined NMP + EMF exposure, alongside…
and Exposed to Wi-Fi Radiofrequency Electromagnetic Radiation Show Enhanced Growth and Lactic Acid Production.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro experimental study exposed two Lactobacillus probiotic cultures to 2.4 GHz Wi‑Fi RF-EMF from a router for 15–60 minutes and compared them with sham-exposed controls. The authors report increased bacterial growth after 30–60 minutes of exposure, with no growth difference at 15 minutes. Lactic acid…
A Novel In Vitro Device to Deliver Induced Electromagnetic Fields to Cell and Tissue Cultures.
Research
RF Safe Research Library
Jan 1, 2020
This paper presents an in vitro, noncontact solenoid-coil device designed to deliver intermediate-frequency induced electromagnetic fields that generate electric fields in cell and tissue cultures. The system aims to improve field characterization and reduce conductive heating compared with electrode-based approaches…
Microtubular structure impairment after GSM-modulated RF radiation exposure.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study exposed V79 cells to 915 MHz GSM-modulated RF radiation for 1–3 hours at 10–30 V/m (average SARs reported as 0.23–1.6 W/kg). The authors report that 3-hour exposure altered microtubule structure across all tested field strengths and reduced cell growth on the third post-exposure day. They conclude…
Effects of electromagnetic fields treatment on rat critical-sized calvarial defects with a 3D-printed composite scaffold.
Research
RF Safe Research Library
Jan 1, 2020
This animal and in vitro study evaluated electromagnetic fields (EMF) treatment combined with a 3D-printed PLA/HA scaffold seeded with rat bone marrow mesenchymal stem cells (BMSCs) for cranial defect repair. The authors report that EMF enhanced BMSC proliferation and osteogenic differentiation and was partly…
Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study examined 50 Hz, 1 mT ELF-EMF exposure in LPS-treated THP-1 cells for 1–24 hours. The authors report increased HO-1 expression and Nrf2-related signaling, with nuclear enrichment of HO-1 and modulation of SIRT1/NF-κB pathways. They interpret these molecular changes as suggesting a protective,…