Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.
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RF Safe Research Library
Jan 1, 2021
This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…
Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
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RF Safe Research Library
Jan 1, 2021
This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…
Enhancement in the ATP level and antioxidant capacity of under continuous exposure to extremely low-frequency electromagnetic field for multiple generations.
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RF Safe Research Library
Jan 1, 2020
This multi-generation animal study exposed worms continuously to 50 Hz, 3 mT extremely low-frequency electromagnetic fields and assessed outcomes in the 15th generation. The study reports increased body length, higher ATP content and ATP synthase activity, and upregulated ATP-related gene expression in exposed worms.…
Electromagnetic field affects the voltage-dependent potassium channel Kv1.3.
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RF Safe Research Library
Jan 1, 2020
This in vitro study tested whether extremely low-frequency electromagnetic fields (ELF-EMF) modulate the voltage-gated potassium channel Kv1.3 in Kv1.3-expressing CHO-K1 cells. Cells were exposed to a 20 Hz field at 268 µT or 902 µT while Kv1.3 currents were measured by whole-cell patch clamp. The authors report a…
Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats.
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RF Safe Research Library
Jan 1, 2020
This rat study examined chronic extremely low-frequency electromagnetic field (ELF-EMF) exposure and diaphragm muscle function and structure. Rats were exposed to 50 Hz, 1.5 mT for 4 hours daily for up to 7 months and assessed with electrophysiological, biochemical, and electron-microscopy measures. The authors…
The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.
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RF Safe Research Library
Jan 1, 2020
This in vitro study tested whether 50 Hz extremely low-frequency electromagnetic fields (0.5 or 1 mT) affect human dental pulp stem cell survival and proliferation. Using MTT and DAPI assays after 7 days of exposure (20 or 40 min/day), all exposed groups reportedly showed higher viability/proliferation than controls.…
Immune response of mollusk Onchidium struma to extremely low-frequency electromagnetic fields (ELF-EMF, 50 Hz) exposure based on immune-related enzyme activity and De novo transcriptome analysis.
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RF Safe Research Library
Jan 1, 2020
This animal study exposed the mollusk Onchidium struma to 50 Hz ELF-EMF at 100 μT or 500 μT for up to one week and assessed immune-related cell counts, enzyme activities, and transcriptomic changes. The 100 μT group showed increased total coelomocyte and spherulocyte density versus control and 500 μT, while some cell…
Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway.
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RF Safe Research Library
Jan 1, 2020
This in vitro study exposed human dermal papilla cells to 70 Hz extremely low-frequency electromagnetic fields at 5–100 G for four days. The abstract reports increased expression of several anagen-related molecules and increased proliferation, with 10 G described as the most potent condition. It also reports…
Lipidomic alteration and stress-defense mechanism of soil nematode Caenorhabditis elegans in response to extremely low-frequency electromagnetic field exposure.
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RF Safe Research Library
Jan 1, 2019
This animal (nematode) study exposed Caenorhabditis elegans to 50 Hz, 3 mT extremely low-frequency EMF and assessed multi-omics responses. The authors report significant lipidomic, proteomic, and transcriptomic alterations, including increased triacylglycerols. Pathway analyses suggest disrupted lipid metabolism,…
T-Cell Differentiation to T Helper 9 Phenotype is Elevated by Extremely Low-Frequency Electromagnetic Fields Via Induction of IL-2 Signaling.
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Jan 1, 2019
This in vitro study examined 60 Hz extremely low-frequency EMF exposure (0.8 mT) on CD4 T cells under Th9-polarizing conditions. The authors report increased Th9-associated responses including IL-9 secretion, transcription factor changes (including early GATA3), and increased STAT5/STAT6 phosphorylation alongside…
Impact of extremely low-frequency electromagnetic field (100 Hz, 100 G) exposure on human glioblastoma U87 cells during Temozolomide administration.
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RF Safe Research Library
Jan 1, 2019
This in vitro study evaluated whether extremely low-frequency EMF exposure (100 Hz, 100 G) modifies the effects of temozolomide (100 µM) in human glioblastoma U87 cells over 120–144 hours. The authors report that combined treatment reduced stemness-associated markers (Nestin, CD133, Notch4) and increased GFAP…
Correlation between the Lunar Phase and Tail-Lifting Behavior of Lizards () Exposed to an Extremely Low-Frequency Electromagnetic Field.
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RF Safe Research Library
Jan 1, 2019
This animal study examined long-term tail-lifting behavior in agamid lizards exposed to an ELF electromagnetic field (6 and 8 Hz; peak magnetic field 2.6 µT; peak electric field 10 V/m) versus controls, incorporating lunar phase and geomagnetic activity (K index) as explanatory variables. Regression analyses found…
Geobacter sulfurreducens-inoculated bioelectrochemical system reveals the potential of metabolic current in defining the effect of extremely low-frequency electromagnetic field on living cells.
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RF Safe Research Library
Jan 1, 2019
This laboratory study exposed a Geobacter sulfurreducens-inoculated bioelectrochemical system to 0–128 Gauss extremely low-frequency electromagnetic fields. The authors report increased and oscillatory metabolic current under ELF-EMF exposure, with dose-dependent effects and a linear relationship between oscillation…
Synergistic effect of rice bran extract and extremely low-frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis.
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RF Safe Research Library
Jan 1, 2018
This in vitro study used a 3D dermal papilla-like tissue model containing dermal papilla cells and melanocytes to assess melanogenesis after rice bran extract (RBE), extremely low-frequency EMF, or their combination. The combined RBE/EMF condition showed higher tyrosinase activity, increased melanogenesis-related…
Increase in Blood Levels of Growth Factors Involved in the Neuroplasticity Process by Using an Extremely Low Frequency Electromagnetic Field in Post-stroke Patients.
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Jan 1, 2018
This study evaluated whether adding extremely low-frequency electromagnetic field (ELF-EMF) therapy to standard post-stroke rehabilitation affects biomarkers of neuroplasticity and functional recovery. Forty-eight patients completed the same rehabilitation program, with the study group additionally receiving 10…
Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal Cells.
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RF Safe Research Library
Jan 1, 2018
This in vitro study evaluated whether a therapeutic extremely low-frequency EMF exposure (5 Hz, 0.4 mT) causes cytotoxic or genotoxic effects in a human mesenchymal stromal cell line. Cells were exposed for 20 minutes per day, three times per week, for two weeks and assessed for viability, proliferation, and…
Effects of Fifty-Hertz Electromagnetic Fields on Granulocytic Differentiation of ATRA-Treated Acute Promyelocytic Leukemia NB4 Cells.
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Jan 1, 2018
This in vitro study examined whether 50 Hz, 2 mT extremely low-frequency electromagnetic fields affect ATRA-driven granulocytic differentiation in NB4 acute promyelocytic leukemia cells. The authors report reduced proliferation and enhanced maturation/differentiation markers with ELF-EMF exposure. They also report…
Case-Control Study on Occupational Exposure to Extremely Low-Frequency Electromagnetic Fields and the Association with Meningioma.
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RF Safe Research Library
Jan 1, 2018
This case-control analysis assessed occupational extremely low-frequency electromagnetic field (ELF-EMF) exposure and meningioma using the INTEROCC ELF-EMF job-exposure matrix. Cumulative, average, and maximum job exposure metrics were evaluated. The study reports no increased meningioma risk in any exposure…
Extremely low-frequency electromagnetic fields accelerates wound healing modulating MMP-9 and inflammatory cytokines.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study used a scratch wound model in human immortalized keratinocytes (HaCaT) to examine effects of extremely low-frequency electromagnetic fields (ELF-EMF). ELF-EMF exposure was reported to accelerate wound closure compared with sham and to increase inflammatory cytokine expression/release. The authors…
Extremely low-frequency electromagnetic field (EMF) generates alterations in the synthesis and secretion of oestradiol-17β (E) in uterine tissues: An in vitro study.
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RF Safe Research Library
Jan 1, 2018
This in vitro study exposed porcine endometrial and myometrial slices to extremely low-frequency EMF (50 or 120 Hz, 8 mT) for 2 or 4 hours, with or without progesterone. The authors report frequency- and time-dependent changes in oestradiol-17β secretion and CYP19A3 mRNA expression, with fewer changes when…
Effect of extremely low-frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544.
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RF Safe Research Library
Jan 1, 2017
This in vitro study exposed human keratinocyte cells (NCTC 2544) to 50 Hz ELF-EMF at 0.25–2 G and assessed oxidative stress-related endpoints. Significant increases in ROS were reported at 0.5 and 1 G, with associated short-term changes in antioxidant and lipid peroxidation markers. The authors report that…
Benign Effect of Extremely Low-Frequency Electromagnetic Field on Brain Plasticity Assessed by Nitric Oxide Metabolism during Poststroke Rehabilitation.
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RF Safe Research Library
Jan 1, 2017
This study assessed whether adding extremely low-frequency electromagnetic field (ELF-EMF) exposure to a 4-week poststroke rehabilitation program affects nitric oxide (NO)-related biomarkers and patient status. Forty-eight poststroke patients were assigned to ELF-EMF versus non-ELF-EMF rehabilitation, with the…
Cytotoxicity of temozolomide on human glioblastoma cells is enhanced by the concomitant exposure to an extremely low-frequency electromagnetic field (100Hz, 100G).
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study tested whether an extremely low-frequency EMF (100 Hz, 100 G) could enhance temozolomide cytotoxicity in human glioma cell lines (U87 and T98G). The authors report that combined EMF and temozolomide increased apoptosis relative to either treatment alone and was accompanied by changes in…
Effect of electromagnetic fields and antioxidants on the trace element content of rat teeth.
Research
RF Safe Research Library
Jan 1, 2017
This animal study examined whether extremely low-frequency electromagnetic fields (ELF-EMFs) from a high-voltage source alter trace element concentrations in rat teeth and whether antioxidants (melatonin and glutathione) modify these changes. Rats were exposed 8 hours/day for 26 or 52 days, with separate sham…