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Inactivation of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus brevis in Low-fat Milk by Pulsed Electric Field Treatment: A Pilot-scale Study.

Research RF Safe Research Library Jan 1, 2015

This pilot-scale study evaluated pulsed electric field (PEF) processing of low-fat milk inoculated with E. coli, S. cerevisiae, or L. brevis. At 10 kV/cm and 200 kJ/L total pulse energy, the study reports multi-log microbial reductions, with >5-log reductions during storage at 200–250 kJ/L and slow regrowth over 15…

Electromagnetic radiation (Wi-Fi) and epilepsy induce calcium entry and apoptosis through activation of TRPV1 channel in hippocampus and dorsal root ganglion of rats

Research RF Safe Research Library Jan 1, 2014

This animal study examined 2.45 GHz Wi-Fi exposure in a PTZ-induced epilepsy rat model using freshly isolated hippocampal and DRG neurons. The authors report that epilepsy increased Ca(2+) influx, oxidative stress markers, and apoptosis-related outcomes, and that Wi-Fi exposure produced additional increases in…

Electromagnetic fields act via activation of voltage-gated calcium channels to produce beneficial or adverse effects

Research RF Safe Research Library Jan 1, 2013

This narrative review argues that non-thermal biological effects of extremely low and microwave frequency EMFs may be mediated by activation of voltage-gated calcium channels (VGCCs). It cites 23 studies in which VGCC blockers reportedly block or reduce diverse EMF effects and proposes downstream…

[Modulation of Ca(2+)-dependent proteinase activity in invertebrates and fish under the action of weak low-frequency magnetic fields].

Research RF Safe Research Library Jan 1, 2013

This study examined whether weak low-frequency magnetic fields affect Ca2+-dependent proteinases (calpains) in fish and invertebrates using in vivo and in vitro experiments. The authors report a significant decrease in calpain activity in animals exposed to a field tuned to Ca2+ parametric resonance, and substantial…

Nanosecond pulse electric field activation of platelet-rich plasma reduces myocardial infarct size and improves left ventricular mechanical function in the rabbit heart.

Research RF Safe Research Library Jan 1, 2012

This animal and in vitro study evaluated nanosecond pulsed electric fields (nsPEF) as a nonchemical method to activate platelet-rich plasma (PRP) for cardioprotection. In rabbits undergoing brief coronary occlusion followed by reperfusion, PRP injection into ischemic myocardium was associated with reduced infarct…

Transient and steady-state magnetic fields induce increased fluorodeoxyglucose uptake in the rat hindbrain.

Research RF Safe Research Library Jan 1, 2011

This animal PET study examined whether a weak 60-Hz magnetic field alters brain glucose utilization in rats. The abstract reports increased FDG uptake in hindbrain voxels when a 2.5-G field was oriented orthogonally to the sagittal plane, but not when field direction varied randomly. Distinct activation effects were…

In vitro magnetic stimulation of pig phrenic nerve with transverse and longitudinal induced electric fields: analysis of the stimulation site.

Research RF Safe Research Library Jan 1, 2009

This in vitro study analyzed where magnetic stimulation activates pig phrenic nerves when induced electric fields are oriented longitudinally versus transversely to the nerve trunk. Longitudinal-field stimulation localized activation near the negative peak of the spatial derivative of the longitudinal electric field,…

Role of cortical cell type and morphology in subthreshold and suprathreshold uniform electric field stimulation in vitro.

Research RF Safe Research Library Jan 1, 2009

This in vitro study examined how cortical neuron morphology and cell type relate to responses under subthreshold and suprathreshold uniform electric fields in rat motor cortex slices. The authors report that morphology correlates with subthreshold somatic polarization and that layer V pyramidal neurons are predicted…

Combined effect of temperature and pulsed electric fields on apple juice peroxidase and polyphenoloxidase inactivation.

Research RF Safe Research Library Jan 1, 2008

This laboratory study evaluated pulsed electric fields (PEF) combined with preheating to inactivate peroxidase (POD) and polyphenoloxidase (PPO) in freshly prepared apple juice. The highest reported inactivation occurred with preheating to 50°C and PEF at 40 kV/cm for 100 μs, exceeding the reductions seen with…

[Fluorescence spectra analysis of papain treated by pulsed electric field].

Research RF Safe Research Library Jan 1, 2007

This in vitro study used fluorescence excitation, emission, and polarization spectra to assess structural changes in papain after pulsed electric field (PEF) treatment. Under the reported PEF conditions (50 kV/cm, 1500 Hz, 40 µs pulses, 19,800 pulses), papain activity decreased by 56.5%. Spectral changes (increased…

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