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Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway.

Research RF Safe Research Library Jan 1, 2020

This in vitro study exposed human dermal papilla cells to 70 Hz extremely low-frequency electromagnetic fields at 5–100 G for four days. The abstract reports increased expression of several anagen-related molecules and increased proliferation, with 10 G described as the most potent condition. It also reports…

Exposure to 50 Hz electromagnetic fields enhances hair follicle regrowth in C57BL/6 mice.

Research RF Safe Research Library Jan 1, 2019

This animal study in C57BL/6 mice reports that exposure to 50 Hz EMF affected hair follicle regrowth. It also reports increased proliferation of K15 stem cells in hair follicle regions. The authors interpret the findings as potentially beneficial for healing hair loss, but key methodological details are not provided…

Global gene expression changes reflecting pleiotropic effects of Irpex lacteus induced by low--intensity electromagnetic field.

Research RF Safe Research Library Jan 1, 2019

This laboratory study used RNA-seq to examine how low-intensity electromagnetic field (LI-EMF) exposure affects global gene expression in the fungus Irpex lacteus. Thousands of differentially expressed genes were reported immediately after exposure, with fewer changes after 3 h and 6 h recovery, and a subset of genes…

Non-Ionizing Radiation for Cardiac Human Amniotic Mesenchymal Stromal Cell Commitment: A Physical Strategy in Regenerative Medicine.

Research RF Safe Research Library Jan 1, 2018

This in vitro study exposed human amniotic mesenchymal stromal cells to an ELF-EMF stimulus (7 Hz, 2.5 µT) and compared responses with a nitric oxide chemical treatment. The authors report reduced cell proliferation with no change in metabolic activity, vitality, or apoptosis after ELF-EMF exposure. They also report…

The legacy effects of electromagnetic fields on bone marrow mesenchymal stem cell self-renewal and multiple differentiation potential.

Research RF Safe Research Library Jan 1, 2018

This animal study examined whether electromagnetic field (EMF) pretreatment has lasting (“legacy”) effects on rat bone marrow mesenchymal stem cells (BMSCs) and whether EMF-pretreated BMSC sheets improve fracture nonunion healing in rats. The authors report increased BMSC proliferation and enhanced…

A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels.

Research RF Safe Research Library Jan 1, 2018

This in vitro study examined effects of a 6 mT, 60 Hz uniform ELF electromagnetic field on HeLa cells and primary IMR-90 fibroblasts. Single or repetitive exposure did not induce DNA damage or change viability, but continuous exposure over 168 hours was reported to increase proliferation in both cell types in a…

Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal Cells.

Research RF Safe Research Library Jan 1, 2018

This in vitro study evaluated whether a therapeutic extremely low-frequency EMF exposure (5 Hz, 0.4 mT) causes cytotoxic or genotoxic effects in a human mesenchymal stromal cell line. Cells were exposed for 20 minutes per day, three times per week, for two weeks and assessed for viability, proliferation, and…

Effects of Fifty-Hertz Electromagnetic Fields on Granulocytic Differentiation of ATRA-Treated Acute Promyelocytic Leukemia NB4 Cells.

Research RF Safe Research Library Jan 1, 2018

This in vitro study examined whether 50 Hz, 2 mT extremely low-frequency electromagnetic fields affect ATRA-driven granulocytic differentiation in NB4 acute promyelocytic leukemia cells. The authors report reduced proliferation and enhanced maturation/differentiation markers with ELF-EMF exposure. They also report…

Age-dependent effect of long-term microwave radiation on postnatal neurogenesis in rats: morphological and behavioral study.

Research RF Safe Research Library Jan 1, 2018

This animal study examined pulsed-wave 2.45 GHz microwave radiation exposure (1 or 3 hours daily for 3 weeks) in adult and juvenile rats and assessed neurogenesis-related markers in the rostral migratory stream. The authors report reduced cell proliferation in adults and longer-lasting effects on cell death after…

Biodegradable, pH-sensitive chitosan beads obtained under microwave radiation for advanced cell culture.

Research RF Safe Research Library Jan 1, 2018

This in vitro study describes microwave-assisted, green-chemistry synthesis of pH-sensitive chitosan beads crosslinked with a glutamic acid/1,5-pentanodiol mixture and optionally modified with Tilia platyphyllos extract. The beads were characterized (FT-IR, SEM), tested for swelling, pH sensitivity, antioxidant…

The effects of electromagnetic fields on B16-BL6 cells are dependent on their spatial and temporal character.

Research RF Safe Research Library Jan 1, 2017

This in vitro study tested several complex low-intensity, low-frequency EMF patterns on proliferation of B16-BL6 mouse melanoma cells. A specific pattern (Thomas-EMF) with a 3 ms point duration reportedly inhibited proliferation and increased calcium uptake, while other timing settings did not. The authors conclude…

RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells.

Research RF Safe Research Library Jan 1, 2017

This in vitro study assessed whether intermittent 1800 MHz RF-EMF exposure induces DNA damage or alters cellular behavior in three neurogenic cell lines. Cells were exposed at an average SAR of 4.0 W/kg for 1, 6, or 24 hours and evaluated for γH2AX foci, cell cycle progression, proliferation, and viability. The…

Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells.

Research RF Safe Research Library Jan 1, 2017

This in vitro study tested 50 Hz, 3 mT electromagnetic field (EMF) exposure and 808 nm low-level laser (LLL), alone and combined, on human adipose tissue-derived mesenchymal stem cells from six donors. The authors report increased cell numbers after 7 days with EMF, greater increases with LLL, and the largest…

The expression of pluripotency and neuronal differentiation markers under the influence of electromagnetic field and nitric oxide.

Research RF Safe Research Library Jan 1, 2017

This in vitro study examined how 50 Hz low-frequency EMF and a nitric oxide donor (Deta-NO) affect stemness and neuronal differentiation markers in rat bone marrow mesenchymal stem cells. The authors report that high NO (1 mM) combined with EMF reduced stemness markers and increased neuronal differentiation markers,…

Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells.

Research RF Safe Research Library Jan 1, 2017

This in vitro study evaluated adult murine neural stem cells exposed to GSM 900-MHz radiofrequency emissions from a mobile phone across different exposure durations. The authors report significant decreases in neurosphere number/size and neuronal differentiation with increasing exposure duration. They report no…

Effects of extremely low frequency electromagnetic fields on in-vitro cellular cultures HeLa and CHO.

Research RF Safe Research Library Jan 1, 2016

This in-vitro study examined whether 50 Hz extremely low frequency electromagnetic fields affect proliferation in HeLa and CHO cell cultures. The abstract reports increased proliferation in exposed cells versus controls, with the largest reported increase under repetitive exposure at 2.49 mT (40 minutes every 12…

Effects of Electromagnetic Radiation from Smartphones on Learning Ability and Hippocampal Progenitor Cell Proliferation in Mice.

Research RF Safe Research Library Jan 1, 2016

This mouse study examined chronic smartphone electromagnetic radiation exposure and its effects on spatial working memory and hippocampal progenitor cell proliferation. The authors report no significant differences in Y-maze spontaneous alternation or BrdU-labeled progenitor proliferation after exposure, but…

Effects of a continuous electromagnetic field on wound healing in human airway.

Research RF Safe Research Library Jan 1, 2015

This in vitro study exposed BEAS-2B human bronchial epithelial cells to a 1.8-GHz continuous EMF at SAR 1.0 W/kg and assessed wound-healing related endpoints. EMF exposure decreased cell migration in a scratch assay compared with controls. The authors report no cytotoxicity, no inhibition of proliferation, and no…

[Effects of static magnetic field at different times on the proliferation and differentiation of osteoblasts in vitro].

Research RF Safe Research Library Jan 1, 2012

This in vitro study exposed newborn rat calvarial osteoblasts to a 3.9 mT static magnetic field for varying durations (0–4 hours). The authors report increased cell proliferation and markers of osteoblast differentiation and mineralization versus control, including higher ALP activity, calcium content, and…

Influences of electric field on living cells in a charged water-in-oil droplet under electrophoretic actuation.

Research RF Safe Research Library Jan 1, 2011

This experimental study examined whether strong electric fields used for electrophoretic actuation of charged water-in-oil droplets affect living cells inside the droplets. Using Trypan blue and cell growth tests across electric field strengths of 1–3 kV/cm and varying actuation times, the authors report no…

[Time effect of sinusoidal electromagnetic field on enhancing the maturation and mineralization of osteoblasts in vitro].

Research RF Safe Research Library Jan 1, 2011

This in vitro study exposed newborn rat calvarial osteoblasts to 50 Hz sinusoidal electromagnetic fields at 1.8 mT for different daily exposure times (0.5–3.0 h). The authors report inhibited osteoblast proliferation at longer exposure times, alongside increased differentiation markers (ALP activity) and…

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